Overexpression and Purification of Recombinant Eel Calcitonin and Its Phylogenetic Analysis

Abstract

Calcitonin (CT), a peptide hormone that is widely used for the treatment of osteoporosis, Paget's disease, hypercalcemic shock and chronic pain in terminal cancer patients, is produced by the para-follicular cells of the thyroid gland in mammals and by the ultimobranchial gland of birds and fish. Fish calcitonin, like eel calcitonin (eCT), is more potent and longer lasting than human CT and is one of the many bioactive peptides that require C-terminal amidation for full biological activity. In this study we describe the over-expression and over-production of C-terminal amidated eCT in recombinant Streptomyces avermitilis. A phylogenetic analysis was performed with all the known CT amino acid sequences.

Construction of a Recombinant Eukaryotic Expression Plasmid Containing Human Calcitonin Gene and Its Expression in NIH3T3 Cells

“…However, although there have been some studies on the synthesis of calcitonin [14, 15], there is little information in literature on its application in gene therapy. For this purpose, we constructed a recombinant eukaryotic expression plasmid, pCDNA3.0-Ig κ -hCT, which contains human calcitonin gene and murine Ig κ -chain leader sequence.…”