Overexpressing the ANR1 MADS-Box Gene in Transgenic Plants Provides New Insights into its Role in the Nitrate Regulation of Root Development

Gan, Yinbo; Bernreiter, Andreas; Filleur, Sophie; Abram, Beverley L.; Forde, Brian G.

doi:10.1093/pcp/pcs050

Cited by 106 publications

(85 citation statements)

References 61 publications

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“…- (Gan et al, 2012). In Arabidopsis, nrt2.1 mutants have decreased LR initiation, independently of its transport activity, suggesting a role for NRT2.1 in the control of root development (Remans et al, 2006b).…”

Section: Root Architecture Responses To Nitrate Availabilitymentioning

confidence: 99%

Biotechnology of nutrient uptake and assimilation in plants

et al. 2013

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Plants require a complex balance of mineral nutrients to reproduce successfully. Because the availability of many of these nutrients in the soil is compromised by several factors, such as soil pH, cation presence, and microbial activity, crop plants depend directly on nutrients applied as fertilizers to achieve high yields. However, the excessive use of fertilizers is a major environmental concern due to nutrient leaching that causes water eutrophication and promotes toxic algae blooms. This situation generates the urgent need for crop plants with increased nutrient use efficiency and better-designed fertilization schemes. The plant biology revolution triggered by the development of efficient gene transfer systems for plant cells together with the more recent development of next-generation DNA and RNA sequencing and other omics platforms have advanced considerably our understanding on the molecular basis of plant nutrition and how plants respond to nutritional stress. To date, genes encoding sensors, transcription factors, transporters, and metabolic enzymes have been identified as potential candidates to improve nutrient use efficiency. In addition, the study of other genetic resources, such as bacteria and fungi, allows the identification of alternative mechanisms of nutrient assimilation, which are potentially applicable in plants. Although significant progress in this respect has been achieved by conventional breeding, in this review we focus on the biotechnological approaches reported to date aimed at boosting the use of the three most limiting nutrients in the majority of arable lands: nitrogen, phosphorus, and iron.

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Section: Root Architecture Responses To Nitrate Availabilitymentioning

confidence: 99%

Biotechnology of nutrient uptake and assimilation in plants

et al. 2013

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“…For instance, an Arabidopsis MADS-box gene, ANR1, has been shown to promote the lateral root growth on nitrate-rich patches 2,16,17 . Another Arabidopsis gene for NSR1, which is structurally close to the PHR1 transcription factor that functions in phosphorus starvation responses, has been identified as a nitrogen starvation-inducible gene 18 .…”

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confidence: 99%

Arabidopsis NIN-like transcription factors have a central role in nitrate signalling

2013

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In plants, nitrate is not only a major nitrogen source but also a signalling molecule that modulates the expression of a wide range of genes and that regulates growth and development. The critical role of nitrate as a signalling molecule has been established for several decades. However, the molecular mechanisms underlying the nitrate response have remained elusive, as the transcription factor that primarily responds to nitrate signals has not yet been identified. Here we show that Arabidopsis NIN-LIKE PROTEIN (NLP) family proteins bind the nitrate-responsive cis-element and activate nitrate-responsive cis-element-dependent and nitrate-responsive transcription. Our results also suggest that the activity of NLPs is posttranslationally modulated by nitrate signalling. Furthermore, the suppression of NLP function impairs the nitrate-inducible expression of a number of genes and causes severe growth inhibition. These results indicate that NLPs are the transcription factors mediating the nitrate signal and thereby function as master regulators of the nitrate response.

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“…Local signaling is mediated by NRT1.1 (NPF6.3) and its downstream MADS-box transcription factor ANR1 (21,23,(31)(32)(33). NRT1.1 is induced by auxin (34) and is itself an auxin transporter that represses lateral root growth in regions of low nitrate (31).…”

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Nitrate foraging by Arabidopsis roots is mediated by the transcription factor TCP20 through the systemic signaling pathway

Guan

Wang

Nacry

et al. 2014

Proc. Natl. Acad. Sci. U.S.A.

190

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To compete for nutrients in diverse soil microenvironments, plants proliferate lateral roots preferentially in nutrient-rich zones. For nitrate, root foraging involves local and systemic signaling; however, little is known about the genes that function in the systemic signaling pathway. By using nitrate enhancer DNA to screen a library of Arabidopsis transcription factors in the yeast one-hybrid system, the transcription factor gene TEOSINTE BRANCHED1/CYCLO-IDEA/PROLIFERATING CELL FACTOR1-20 (TCP20) was identified. TCP20, which belongs to an ancient, plant-specific gene family that regulates shoot, flower, and embryo development, was implicated in nitrate signaling by its ability to bind DNA in more than 100 nitrate-regulated genes. Analysis of insertion mutants of TCP20 showed that they had normal primary and lateral root growth on homogenous nitrate media but were impaired in preferential lateral root growth (root foraging) on heterogeneous media in split-root plates. Inhibition of preferential lateral root growth was still evident in the mutants even when ammonium was uniformly present in the media, indicating that the TCP20 response was to nitrate. Comparison of tcp20 mutants with those of nlp7 mutants, which are defective in local control of root growth but not in the root-foraging response, indicated that TCP20 function is independent of and distinct from NLP7 function. Further analysis showed that tcp20 mutants lack systemic control of root growth regardless of the local nitrate concentrations. These results indicate that TCP20 plays a key role in the systemic signaling pathway that directs nitrate foraging by Arabidopsis roots. TCP20 | nitrate | root foraging | systemic signaling | Arabidopsis

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Overexpressing the ANR1 MADS-Box Gene in Transgenic Plants Provides New Insights into its Role in the Nitrate Regulation of Root Development

Cited by 106 publications

References 61 publications

Biotechnology of nutrient uptake and assimilation in plants

Biotechnology of nutrient uptake and assimilation in plants

Arabidopsis NIN-like transcription factors have a central role in nitrate signalling

Nitrate foraging by Arabidopsis roots is mediated by the transcription factor TCP20 through the systemic signaling pathway

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