Overexpressing key component genes of the secretion pathway for enhanced secretion of an Aspergillus niger glucose oxidase in Trichoderma reesei

Wu, Yilan; Sun, Xianhua; Xue, Xianli; Luo, Huiying; Yao, Bin; Xie, Xiangming; Su, Xiaoyun

doi:10.1016/j.enzmictec.2017.07.007

Cited by 41 publications

(35 citation statements)

References 26 publications

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“…Of particular note, manipulation of certain gene may cause quite different effects in different strains. For example, overexpression of bip1 promoted protein secretion in T. reesei (Wu et al, 2017) and A. awamori (Lombraña et al, 2004), while reduced protein secretion was observed in A. niger by adopting the same strategy (Conesa et al, 2002). These effects could be attributed to the multifunction of BiP.…”

Section: Regulation Of Upr and Erad To Promote Protein Secretionmentioning

confidence: 98%

“…For example, overexpression of the transcription factor hac1 in Aspergillus awamori led to 7-and 2.8fold increases in laccase and bovine prechymotrypsin production, respectively (Valkonen et al, 2003). Overexpression of bip1 and hac1 in T. reesei exhibited 1.5-and 1.8-fold improvement on secretion of an A. niger glucose oxidase (Wu et al, 2017).…”

Section: Regulation Of Upr and Erad To Promote Protein Secretionmentioning

confidence: 99%

“…A G-to-T mutation at nucleotide #751 in podB1 led to significant increase in cellulase and xylanase activities (Boppidi et al, 2018). Wu et al overexpressed snc1 gene, which is involved in fusion of vesicles and plasma membrane, and observed a 2.2-fold increase in secretion of an A. niger glucose oxidase in T. reesei (Wu et al, 2017). In addition to being successfully secreted outside, some heterologous proteins may be transported to the vacuole for degradation (Masai et al, 2003).…”

Section: Optimization Of the Intracellular Transport Processmentioning

confidence: 99%

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Genetic Engineering of Filamentous Fungi for Efficient Protein Expression and Secretion

Wang

Zhong

Xiao

2020

Front. Bioeng. Biotechnol.

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Filamentous fungi are considered as unique cell factories for protein production due to the high efficiency of protein secretion and superior capability of post-translational modifications. In this review, we firstly introduce the secretory pathway in filamentous fungi. We next summarize the current state-of-the-art works regarding how various genetic engineering strategies are applied for enhancing protein expression and secretion in filamentous fungi. Finally, in a future perspective, we discuss the great potential of genome engineering for further improving protein expression and secretion in filamentous fungi.

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Section: Regulation Of Upr and Erad To Promote Protein Secretionmentioning

confidence: 98%

Section: Regulation Of Upr and Erad To Promote Protein Secretionmentioning

confidence: 99%

Section: Optimization Of the Intracellular Transport Processmentioning

confidence: 99%

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Genetic Engineering of Filamentous Fungi for Efficient Protein Expression and Secretion

Wang

Zhong

Xiao

2020

Front. Bioeng. Biotechnol.

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“…The commercially available first generation enzyme preparations (like Spezyme CP, Celluclast, and Novozyme 188) have been found to be underperforming in hydrolyzing the xylo-oligomers and overcoming their inhibitory effects at reasonable dosages (Qing et al, 2010;Agrawal et al, 2015b). This is due to the fact that these commercial enzymes are usually derived from the filamentous fungi (Trichoderma) and contains high amount of exo and endo glucanases but with lower amounts accessory enzymes like (β-glucosidases and hemicellulose) (Agrawal et al, 2015b;Pierce et al, 2017;Wu et al, 2017). In addition to this, type of ligno-cellulosic biomass and the pretreatment approach bring in differences in their structural properties (Satlewal et al, 2018b).…”

Section: Introductionmentioning

confidence: 99%

Synergistic Enzyme Cocktail to Enhance Hydrolysis of Steam Exploded Wheat Straw at Pilot Scale

et al. 2018

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Multiple enzymes are required for efficient hydrolysis of lignocellulosic biomass and no wild type organism is capable of producing all enzymes in desired levels. In this study, steam explosion of wheat straw was carried out at pilot scale and a synthetic enzyme mixture (EnzMix) was developed by partially replacing the cellulase with critical dosages of commercially available accessory enzymes (β-glucosidase, xylanase and laccase) through central composite design. Highest degree of synergism (DS) was observed with β-glucosidase (1.68) followed by xylanase (1.36). Finally, benchmarking of EnzMix (Celluclast, β-glucosidase and xylanase in a protein ratio of 20.40: 38.43: 41.16, respectively) and other leading commercial enzymes was carried out. Interestingly, hydrolysis improved by 75% at 6 h and 30% at 24 h, respectively in comparison of control. By this approach, 25% reduction in enzyme dosage was observed for obtaining the same hydrolysis yield with opitimized enzyme cocktail. Thus, development of enzyme cocktail is an effective and sustainable approach for high hydrolysis efficiency.

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“…After the discovery of transformation techniques [37,38], various proteins of fungal and non-fungal origin have been successfully expressed in T. reesei [39,40]; the production of fungal proteins, in particular, was more efficient than that of mammalian proteins. For instance, fungal proteins such as laccase, lipase, xylanase, and glucose oxidase were expressed using the cbh1 promoter at levels of 100 mg/L and several grams per liter in shake-flask and fermentation cultures, respectively [26,[41][42][43]. One of the most advantageous properties of T. reesei is that it does not hyperglycosylate proteins.…”

Section: Discussionmentioning

confidence: 99%

Improvement of Saccharification and Delignification Efficiency of Trichoderma reesei Rut-C30 by Genetic Bioengineering

et al. 2020

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Trichoderma reesei produces various saccharification enzymes required for biomass degradation. However, the lack of an effective lignin-degrading enzyme system reduces the species’ efficiency in producing fermentable sugars and increases the pre-treatment costs for biofuel production. In this study, we heterologously expressed the Ganoderma lucidum RMK1 versatile peroxidase gene (vp1) in the Rut-C30 strain of T. reesei. The expression of purified 6×His-tag–containing recombinant G. lucidum-derived protein (rVP1) was confirmed through western blot, which exhibited a single band with a relative molecular weight of 39 kDa. In saccharification and delignification studies using rice straw, the transformant (tVP7, T. reesei Rut-C30 expressing G. lucidum-derived rVP1) showed significant improvement in the yield of total reducing sugar and delignification, compared with that of the parent T. reesei Rut-C30 strain. Scanning electron microscopy (SEM) of tVP7-treated paddy straw showed extensive degradation of several layers of its surface compared with the parent strain due to the presence of G. lucidum-derived rVP1. Our results suggest that the expression of ligninolytic enzymes in cellulase hyperproducing systems helps to integrate the pre-treatment and saccharification steps that may ultimately reduce the costs of bioethanol production.

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Overexpressing key component genes of the secretion pathway for enhanced secretion of an Aspergillus niger glucose oxidase in Trichoderma reesei

Cited by 41 publications

References 26 publications

Genetic Engineering of Filamentous Fungi for Efficient Protein Expression and Secretion

Genetic Engineering of Filamentous Fungi for Efficient Protein Expression and Secretion

Synergistic Enzyme Cocktail to Enhance Hydrolysis of Steam Exploded Wheat Straw at Pilot Scale

Improvement of Saccharification and Delignification Efficiency of Trichoderma reesei Rut-C30 by Genetic Bioengineering

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