Overexpressed microRNA-141-3p enhance proliferation via targeting PLAG1 in non-diabetic macrosomia

Abstract

31Several studies have shown microRNAs (miRNAs) could regulate the placental 32 development, yet the role and mechanism of miRNAs in the development of 33 non-diabetic macrosomia (NDFMS) remains unclear. The key miRNA that abnormal 34 expressed in NDFMS placentas was screened out by miRNA microarray and verified 35 using qRT-PCR in 91 subjects. The effects of the key miRNA were verified by 36 proliferation assay and invasion assay in HTR-8/SVneo cell, and also in pregnant 37 C57BL/6J mice. miR-141-3p was determined as the key miRNA with the most 38 significant difference, which could promote the proliferation and invasion by 39 regulating the expression of target gene PLAG1. Overexpression of PLAG1 could 40 reverse the effect of cell proliferation and invasion ability caused by miR-141-3p 41 overexpression. Significant difference in fetal birth weight was observed between the 42 control group and treated group with miR-141-3p agomir in late pregnancy, but not in 43 early pregnancy. This study revealed miR-141-3p could increase the proliferation of 44 placenta to participate in the occurrence and development of NDFMS through 45 regulating PLAG1 expression.46