“…The most used protocols are (square-wave) incremental protocols with and without resting in between the stages with different starting velocities, increments or duration of stages, or protocols based on individual performance, e.g., starting speed related to theoretical maximal heart rate [6, 7, 10, 12-14, 32, 34-37, 39, 42-46, 48-50, 52]. The de nitions of MAS used in the studies were also differente.g., the rst speed when VO 2 max occurred with no further speci cation [6, 39,45,46,48,49,52], calculated based on VO 2 kinetics [7,32,34,35,43,44,50]the rst speed of the 30-s interval of VO 2 max [7,12,13,50], the speed at the onset of the plateau of VO 2 [10,42], or the nal speed reached during CPET [14,[35][36][37]. Regarding validity of CPET on a treadmill to determine MAS, different incremental continuous protocols yielded similar results when the increments and stage durations were multiples of each other (e.g.…”