Overestimation of 25-hydroxyvitamin D3 by increased ionisation efficiency of 3-epi-25-hydroxyvitamin D3 in LC–MS/MS methods not separating both metabolites as determined by an LC–MS/MS method for separate quantification of 25-hydroxyvitamin D3, 3-epi-25-hydroxyvitamin D3 and 25-hydroxyvitamin D2 in human serum

Ouweland, Johannes M.W. van den; Beijers, Antonius M.; Daal, Henny van

doi:10.1016/j.jchromb.2014.07.021

Cited by 74 publications

(56 citation statements)

References 20 publications

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“…Its clinical significance is unknown but it could be erroneously measured as 25OHD even with liquid chromatographytandem mass spectrometry [19,20]. Nevertheless, in a recent study on the time-course analysis of 3-epi-25-hydoxyvitamin D3 in infants, 3-epi-25-hydroxyvitamin D3 level normalized to <10% in infants older than 3 months of age.…”

Section: Discussionmentioning

confidence: 99%

Vitamin D Status in Premature Infants at Risk of Metabolic Bone Disease of Prematurity

J¹,

Ac²,

Kelly³

2017

Matern Pediatr Nutr

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Objectives: The purpose of this study is to report the vitamin D status in a group of premature infants with increased risk of MBD and to describe the relationship between their vitamin D status and intake.Methods: Retrospective descriptive study in in a level IV neonatal intensive care unit. Results:One hundred and fifty-two subjects were included in the study. Mean gestational age was 26.8 ± 3.3 weeks and mean age was 157 ± 93 days. Mean serum 25OHD concentration was 57.8 ± 2.0 mg/mL. The prevalence of 25OHD <20 ng/mL and 25OHD >100 ng/mL was 5.9% (n=9) and 8.6% (n=13), respectively. No association between 25OHD level and vitamin D intake with respect to total daily dose (p=0.43) or total daily dose based on body weight (p=0.812) was found. Younger gestational age and younger chronological age were associated with wider range of 25OHD levels. Conclusions:Both suboptimal and elevated 25OHD concentrations are found with our current protocol for supplementation. Vitamin D status in premature infants does not correlate with their vitamin D intake, but younger gestational age and chronological age are both associated with more diverse range of 25OHD.

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Section: Discussionmentioning

confidence: 99%

Vitamin D Status in Premature Infants at Risk of Metabolic Bone Disease of Prematurity

J¹,

Ac²,

Kelly³

2017

Matern Pediatr Nutr

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“…In addition, 37 single patient donor serum samples were obtained by drawing an extra tube of blood from patients who already underwent a venipuncture for diagnostic purposes in our outpatient clinic (VU University Medical Center, Amsterdam). These patient sera had concentrations ranging from <1 to 134 nmol/L for 25(OH)D 3 , < 2 to 27 nmol/L for 25(OH)D 2 and < 1 to 7 nmol/L for epi25(OH)D 3 as determined by [12]. All samples were anonymized immediately after withdrawal and processed like regular patient samples.…”

Section: Methodsmentioning

confidence: 99%

Various calibration procedures result in optimal standardization of routinely used 25(OH)D ID-LC-MS/MS methods

Dirks

Vesper

Herwaarden

et al. 2016

Clinica Chimica Acta

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Background The variety of LC-MS/MS methods measuring total 25(OH)D used today is vast and the comparability among these methods is still not well assessed. Methods Here, we performed a comparison in samples of healthy donors between the currently routinely used 25(OH)D LC-MS/MS methods in the Netherlands and the Ghent University reference measurement procedure to address this issue (n = 40). Additionally, an interlaboratory comparison in patient serum samples assessed agreement between the Dutch diagnostic methods (n = 37). Results The overall correlation of the routine methods for 25(OH)D3 with the reference measurement procedures and with the mean of all diagnostic methods was excellent (r > 0.993 and r > 0.989, respectively). Three out of five methods aligned perfectly with both the reference measurement procedure and the median of all methods. One of the routine methods showed a small positive bias, while another showed a small negative bias consistently in both comparisons. Conclusion The biases most probably originated from differences in calibration procedure and may be obviated by reassessing calibration of stock standards and/or calibrator matrices. In conclusion, five diagnostic centers have performed a comparison with the 25(OH)D Ghent University reference measurement procedure in healthy donor serum samples and a comparison among themselves in patient serum samples. Both analyses showed a high correlation and specificity of the routine LC-MS/MS methods, yet did reveal some small standardization issues that could not be traced back to the technical details of the different methods. Hence, this study indicates various calibration procedures can result in perfect alignment.

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“…Figure S1 (Supplementary Material) illustrates the separation of 25(OH)D 3 and 3-epi-25(OH)D 3 for a CLD patient sample, showing~5% contribution of the epimer relative to the 25(OH)D 3 signal. Note that the peak areas of the two epimers are not directly comparable because of significant response factor differences between the two epimer species, as recently described by van den Ouweland et al [25] and Flynn et al [26], requiring a dedicated stable isotope standard for 3-epi-25(OH)D 3 [12].…”

Section: Hrms: Tof-ms Versus Tof-ms/msmentioning

confidence: 95%

“…Although the instrumental resolving power of the QqTOF initially appeared to be sufficiently high, there was a small overlap of the main interference at m/z 401.2666 (C 21 H 33 N 6 O 2 + ; mass measurement accuracy, 1.5 ppm; the same interference was previously observed in the isobaric space of m/z 401 by FTICR analysis of serum samples [6]) from the tail-end of the peak into the 25(OH)D 3 signal. This effect caused isobaric cross-talk from the slightly skewed peaks, which then irreproducibly co-contributed to signal intensities of 25 + ion at m/z 401.3414 and no interference occurred [18]. This separation is particulary important for those targeted assays that include vitamin D 2 species in the acquisition routine.…”

Section: Hrms: Tof-ms Versus Tof-ms/msmentioning

confidence: 99%

Triple Quadrupole Versus High Resolution Quadrupole-Time-of-Flight Mass Spectrometry for Quantitative LC-MS/MS Analysis of 25-Hydroxyvitamin D in Human Serum

Geib

Sleno

Hall

et al. 2016

J. Am. Soc. Mass Spectrom.

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Abstract. We describe a systematic comparison of high and low resolution LC-MS/ MS assays for quantification of 25-hydroxyvitamin D 3 in human serum. Identical sample preparation, chromatography separations, electrospray ionization sources, precursor ion selection, and ion activation were used; the two assays differed only in the implemented final mass analyzer stage; viz. high resolution quadrupolequadrupole-time-of-flight (QqTOF) versus low resolution triple quadrupole instruments. The results were assessed against measured concentration levels from a routine clinical chemiluminescence immunoassay. Isobaric interferences prevented the simple use of TOF-MS spectra for extraction of accurate masses and necessitated the application of collision-induced dissociation on the QqTOF platform. The two mass spectrometry assays provided very similar analytical figures of merit, reflecting the lack of relevant isobaric interferences in the MS/MS domain, and were successfully applied to determine the levels of 25-hydroxyvitamin D for patients with chronic liver disease.

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Cited by 74 publications

References 20 publications

Vitamin D Status in Premature Infants at Risk of Metabolic Bone Disease of Prematurity

Vitamin D Status in Premature Infants at Risk of Metabolic Bone Disease of Prematurity

Various calibration procedures result in optimal standardization of routinely used 25(OH)D ID-LC-MS/MS methods

Triple Quadrupole Versus High Resolution Quadrupole-Time-of-Flight Mass Spectrometry for Quantitative LC-MS/MS Analysis of 25-Hydroxyvitamin D in Human Serum

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