Overdrive is a T-region transfer enhancer which stimulates T-strand production in<i>Agrobacterium tumefaciens</i>

Haaren, M. J. J. Van; Sedee, N. J. A.; Schilperoort, R. A.; Hooykaas, Paul J. J.

doi:10.1093/nar/15.21.8983

Cited by 51 publications

(25 citation statements)

References 43 publications

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“…In Agrobacterium plasmid pTi15955, this region contains a 25-bp sequence (overdrive) that has been reported to promote right border activity (van Haaren et al, 1987). Our work demonstrates that the overdrive is part of a larger ACR domain that is conserved among Agrobacterium Ti and Ri plasmids.…”

Section: Discussionmentioning

confidence: 65%

“…This arrangement placed the potato right border alternative at a distance of 12 bp from the overdrive, an element that was reported to promote DNA transfer (van Haaren et al, 1987). Although the overdrive element is believed to function in a position-independent manner with respect to the right border (Shurvinton and Ream, 1991), we found that a single basepair insertion between St02 and upstream DNA reduced transformation frequencies of pSIM579 about 2-fold (Fig.…”

Section: The Influence Of Flanking Dna On the Efficacy Of Right Bordementioning

confidence: 67%

“…DNA transfer is initiated by virD2-mediated cleavage within the 25-bp right border sequence (Wang et al, 1984;Scheiffele et al, 1995). The Agrobacterium plasmids pTiC58 and pRi8196 contain additional sequences in the vicinity of the right border that enhance cleavage (van Haaren et al, 1987;Hansen et al, 1992). Upon initial cleavage, virD2 covalently binds to the 5# side, and the DNA unwinds toward the left border where a second cleavage reaction may occur.…”

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confidence: 99%

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Plant-Derived Transfer DNAs

et al. 2005

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The transfer of DNA from Agrobacterium to plant cell nuclei is initiated by a cleavage reaction within the 25-bp right border of Ti plasmids. In an effort to develop all-native DNA transformation vectors, 50 putative right border alternatives were identified in both plant expressed sequence tags and genomic DNA. Efficacy tests in a tobacco (Nicotiana tabacum) model system demonstrated that 14 of these elements displayed at least 50% of the activity of conventional Agrobacterium transfer DNA borders. Four of the most effective plant-derived right border alternatives were found to be associated with intron-exon junctions. Additional elements were embedded within introns, exons, untranslated trailers, and intergenic DNA. Based on the identification of a single right border alternative in Arabidopsis and three in rice (Oryza sativa), the occurrence of this motif was estimated at a frequency of at least 0.8 3 10 28 . Modification of plasmid DNA sequences flanking the alternative borders demonstrated that both upstream and downstream sequences play an important role in initiating DNA transfer. Optimal DNA transfer required the elements to be preceded by pyrimidine residues interspaced by AC-rich trinucleotides. Alteration of this organization lowered transformation frequencies by 46% to 93%. Despite their weaker resemblance with left borders, right border alternatives also functioned effectively in terminating DNA transfer, if both associated with an upstream A[C/T

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Section: Discussionmentioning

confidence: 65%

Section: The Influence Of Flanking Dna On the Efficacy Of Right Bordementioning

confidence: 67%

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confidence: 99%

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Plant-Derived Transfer DNAs

et al. 2005

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“…Overdrive sequences enhance the transmission of T-strands to plants, although the molecular mechanism of how this occurs remains unknown (131,156,256,291,336,337,345). Early reports suggested that the VirC1 protein binds to the overdrive sequence and may enhance T-DNA border cleavage by the VirD1/VirD2 endonuclease (322).…”

Section: Molecular Basis Of Agrobacterium-mediated Transformation Whamentioning

confidence: 99%

Agrobacterium -Mediated Plant Transformation: the Biology behind the “Gene-Jockeying” Tool

Gelvin

2003

Microbiol Mol Biol Rev

1,139

687

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SUMMARY Agrobacterium tumefaciens and related Agrobacterium species have been known as plant pathogens since the beginning of the 20th century. However, only in the past two decades has the ability of Agrobacterium to transfer DNA to plant cells been harnessed for the purposes of plant genetic engineering. Since the initial reports in the early 1980s using Agrobacterium to generate transgenic plants, scientists have attempted to improve this “natural genetic engineer” for biotechnology purposes. Some of these modifications have resulted in extending the host range of the bacterium to economically important crop species. However, in most instances, major improvements involved alterations in plant tissue culture transformation and regeneration conditions rather than manipulation of bacterial or host genes. Agrobacterium-mediated plant transformation is a highly complex and evolved process involving genetic determinants of both the bacterium and the host plant cell. In this article, I review some of the basic biology concerned with Agrobacterium-mediated genetic transformation. Knowledge of fundamental biological principles embracing both the host and the pathogen have been and will continue to be key to extending the utility of Agrobacterium for genetic engineering purposes.

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“…The T-DNA is flanked by 25-base-pair (bp) direct repeats, termed border sequences (1)(2)(3)(4), which are required in cis for transfer (5,6). Immediately adjacent to the right borders (borders B and D) is another conserved sequence (5'-TAARTYNCTGTRTNTGTTTGT-TTG-3'), called "overdrive," that enhances the efficiency of tumor formation (7,8) even when located several thousand base pairs away from the border (9). Other studies have indicated that the right border is intrinsically more active than the left in promoting DNA transfer (8,10,11) and that transfer is a polar process proceeding from right to left (7,12,13).…”

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Role of the overdrive sequence in T-DNA border cleavage in Agrobacterium.

Toro

Datta

Yanofsky

et al. 1988

Proc. Natl. Acad. Sci. U.S.A.

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The T-DNA of the Ti plasmid of Agrobacterum is flanked by 25-base-pair imperfect direct repeats that are required in cis for transfer to the genome of the plant host. Another sequence, designated overdrive, is located adjacent to the right-border repeats and functions in cis to enhance tumor formation. We have examined the effect of the overdrive sequence on the early steps in T-DNA processing. We report here that overdrive greatly enhances cleavage by the sitespecific endonuclease in Agrobacterium, perhaps by directing the endonuclease to the adjacent border sequences. We also show by a gel mobility-shift assay that overdrive affinitypurified proteins from acetosyringone-induced Agrobacterium cells interact with T-DNA border and overdrive sequences. Further, we show that in vivo the virC operon enhances cleavage at the T-DNA borders, most likely by interaction between the VirCl protein and the overdrive sequence.

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Overdrive is a T-region transfer enhancer which stimulates T-strand production inAgrobacterium tumefaciens

Cited by 51 publications

References 43 publications

Plant-Derived Transfer DNAs

Plant-Derived Transfer DNAs

Agrobacterium -Mediated Plant Transformation: the Biology behind the “Gene-Jockeying” Tool

Role of the overdrive sequence in T-DNA border cleavage in Agrobacterium.

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