Overcoming the Inflammatory Stage of Non-Healing Wounds: In Vitro Mechanism of Action of Negatively Charged Microspheres (NCMs)

Santos‐Vizcaíno, Edorta; Salvador, Aiala; Vairo, Claudia; Igartua, Manoli; Hernández, Rosa María; Corrêa, Luís Henrique; Villullas, Silvia; Gainza, Garazi

doi:10.3390/nano10061108

Cited by 15 publications

(8 citation statements)

References 44 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…This is of great importance in chronic inflammatory conditions, as a transition from the pro-inflammatory phenotype M1 to the regenerative phenotype M2 is pivotal to overcome the pathological state and heal. A failure in this transition can cause poor healing, as in the case of chronic wounds [ 49 ]. Indeed, recent studies have highlighted the importance of MSCs to modulate the macrophage phenotype from M0 and M1 to M2 to kickstart the wound healing process [ 45 , 50 , 51 , 52 , 53 ].…”

Section: Discussionmentioning

confidence: 99%

Human Hair Follicle-Derived Mesenchymal Stromal Cells from the Lower Dermal Sheath as a Competitive Alternative for Immunomodulation

et al. 2022

Self Cite

View full text Add to dashboard Cite

Mesenchymal stromal cells (MSCs) have unique immunomodulatory capacities. We investigated hair follicle-derived MSCs (HF-MSCs) from the dermal sheath, which are advantageous as an alternative source because of their relatively painless and minimally risky extraction procedure. These cells expressed neural markers upon isolation and maintained stemness for a minimum of 10 passages. Furthermore, HF-MSCs showed responsiveness to pro-inflammatory environments by expressing type-II major histocompatibility complex antigens (MHC)-II to a lesser extent than adipose tissue-derived MSCs (AT-MSCs). HF-MSCs effectively inhibited the proliferation of peripheral blood mononuclear cells equivalently to AT-MSCs. Additionally, HF-MSCs promoted the induction of CD4+CD25+FOXP3+ regulatory T cells to the same extent as AT-MSCs. Finally, HF-MSCs, more so than AT-MSCs, skewed M0 and M1 macrophages towards M2 phenotypes, with upregulation of typical M2 markers CD163 and CD206 and downregulation of M1 markers such as CD64, CD86, and MHC-II. Thus, we conclude that HF-MSCs are a promising source for immunomodulation.

show abstract

Section: Discussionmentioning

confidence: 99%

Human Hair Follicle-Derived Mesenchymal Stromal Cells from the Lower Dermal Sheath as a Competitive Alternative for Immunomodulation

et al. 2022

Self Cite

View full text Add to dashboard Cite

show abstract

“…In addition, M2 can also produce precursors for fibroblast activation and collagen synthesis which is necessary for wound healing, namely FGF-2. 37 …”

Section: Discussionmentioning

confidence: 99%

The Role of Recombinant Secretory Leukocyte Protease Inhibitor to CD163, FGF-2, IL-1 and IL-6 Expression in Skin Wound Healing

Munadziroh¹,

Putri²,

Ristiana³

et al. 2022

CCID

View full text Add to dashboard Cite

Background The wound healing process can be optimized through the addition of a biomaterial such as recombinant secretory leukocyte protease inhibitor (rSLPI). The SLPI is a non-glycosylated proteomic material that inhibits protease enzymes and has anti-inflammatory properties, thus accelerating wound healing. This study analyzed the administration of rSLPI doses 0.04 cc and 0.06 cc in skin wound healing on the CD163 expression of macrophages and cytokines such as interleukin 1 (IL-1), interleukin 6 (IL-6) and fibroblast growth factor 2 (FGF-2). Materials and Methods rSLPI produced from Escherichia coli TOP10 as the cloning host, BL21 (DE3) strains as the expression host and pET30a plasmids were used for the expression system construction. The wound was created on Wistar rat dorsal skin, then rSLPI 0.04 cc and 0.06 cc was administered. In the next four days, the back skin was biopsied and stained by immunohistochemistry to analyze the CD163, FGF-2, IL-1 and IL-6 expression. Results The administration of rSLPI increased CD163 and FGF-2 expression dependent on dose (p<0.05). On the other hand, administration of rSLPI decreased IL-1 and IL-6 expression depending on dose (p <0.05). Conclusion The administration of rSLPI is able to accelerate the wound healing process by increasing the CD163 and FGF-2 expression. The cytokines such as IL-1 and IL-6 decreased depending on rSLPI doses.

show abstract

“…In recent years, with the changes in the spectrum of human diseases, the number of patients and the cost of skin wounds have increased significantly. According to statistics, the total annual direct cost of wound treatment in the United States exceeds $25 billion [5]. Repairing wounds quickly and with high quality is challenging.…”

Section: Introductionmentioning

confidence: 99%

Application of Nanomaterial in Hydrogels Related to Wound Healing

Liu

Song

Liu

et al. 2022

Journal of Nanomaterials

View full text Add to dashboard Cite

Traditional dressings used for wound repair, such as gauze, have shortcomings; for example, they cannot provide a suitable microenvironment for wound recovery. Therefore, it is necessary to find a better dressing to overcome shortcomings. Hydrogel provides a suitable wet environment, has good biocompatibility, and has a strong swelling rate to absorb exudate. Nanomaterial in hydrogels has been used to improve their performance and overcome the shortcomings of current hydrogel dressings. Hydrogel dressing can also be loaded with nanodrug particles to exert a better therapeutic effect than conventional drugs and to make the dressing more practical. This article reviews the application of nanotechnology in hydrogels related to wound healing and discusses the application prospects of nanohydrogels. After searching for hydrogel articles related to wound healing, we found that nanomaterial can not only enhance the mechanical strength, antibacterial properties, and adhesion of hydrogels but also achieve sustained drug release. From the perspective of clinical application, these characteristics are significant for wound healing. The combination of nanomaterial and hydrogel is an ideal dressing with broad application prospects for wound healing in the future.

show abstract

Overcoming the Inflammatory Stage of Non-Healing Wounds: In Vitro Mechanism of Action of Negatively Charged Microspheres (NCMs)

Cited by 15 publications

References 44 publications

Human Hair Follicle-Derived Mesenchymal Stromal Cells from the Lower Dermal Sheath as a Competitive Alternative for Immunomodulation

Human Hair Follicle-Derived Mesenchymal Stromal Cells from the Lower Dermal Sheath as a Competitive Alternative for Immunomodulation

The Role of Recombinant Secretory Leukocyte Protease Inhibitor to CD163, FGF-2, IL-1 and IL-6 Expression in Skin Wound Healing

Application of Nanomaterial in Hydrogels Related to Wound Healing

Contact Info

Product

Resources

About