The fatigue tests with 16 hours hold time have been conducted for two rotor steels, 1CrMoV and 2CrMoNiVW at 550°C to investigate their creep-fatigue interaction behaviors, as well as those without hold time for contrast. And two life prediction methods are used to correlate the present creep-fatigue life data. It is shown that a significant reduction in fatigue life is observed with hold time in tension, and it is also observed that 1CrMoV steel exhibits a higher creep-fatigue life than 2CrMoNiVW when strain hold time is 16 hours, and 2CrMoNiVW seems to be more sensitive to hold time influence. In addition, both methods could give satisfactory creep-fatigue life predictions, but with their own limitations.