Overcoming Interspecific Crossing Barriers in Lilium by Ovary and Embryo Culture

Tuyl, J.M. van; Sande, K. van de; Diën, R. van; Straathof, D.; Holsteijn, H.M.C. van

doi:10.17660/actahortic.1990.266.41

Cited by 17 publications

(3 citation statements)

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“…Obtaining Lilium distant hybrids is impossible without overcoming interspecific crossing barriers. Pre-fertilization barriers can be overcome by: the cut style method; (Van Tuyl et al, 1990, 1997Fernández et al, 1996;Roh et al, 1996) the grafted style method (Van Tuyl et al, 1997), embryo isolation, ovary (Asano, 1980;Okazaki et al, 1994) or embryo isolation (Hayashi et al, 1986;Fernández et al, 1996Fernández et al, , 1998Niimi et al, 1996;Van Tuyl and van Holsteijn, 1996) and in vitro fertilization.…”

Section: Introductionmentioning

confidence: 99%

Distant Hybrids and Their Verification by Means of Rapd

Ploszaj¹,

Stojałowski²,

Startek³

et al. 2005

Acta Hortic.

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In Poland, apart from Oriental lilies, Lilium longiflorum and its Asiatic hybrids, have become very popular. As a result of interspecific and intergroup hybridization, progeny plants may not always be hybrids. Embryos may be formed via apomixis and such seedlings contain the genetic material of the maternal form. Because apomixis can occur in Lilium regale, L. longiflorum, L. superbum and L. pumilum, confirmation that the obtained seedlings are hybrids is required. Studies identifying hybrids, based on DNA analysis began in the 1980's. Molecular techniques, with the most popular one-RAPD, are used for identification. As a result of crossing Lilium longiflorum with 'Connecticut King', which is an Asiatic hybrid, progeny plants were obtained using embryo isolation method. The confirmation of their hybrid status was performed by RAPD, analysing DNA genotypes of two parental forms and six progeny plants. DNA was isolated from the leaves of young lilies growing in the greenhouse and 11 primers of 10-nucleotide sequences chosen at random, from LA pr 1 to LA pr 11 were used. Amplified markers by RAPD were separated on agar gel and visualized in UV light in the presence of ethidium bromide. Among evaluated genotypes five turned out to be hybrids and one had the same genotype as the maternal plant.

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Section: Introductionmentioning

confidence: 99%

Distant Hybrids and Their Verification by Means of Rapd

Ploszaj¹,

Stojałowski²,

Startek³

et al. 2005

Acta Hortic.

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“…Embryo rescue or ovule culture techniques have been used to produce new cultivars of flower crops, e.g. Lilium (Van Tuyl et al, 1990, 1991 and Gypsophila (Kishi et al, 1994). A number of interspecific Limonium hybrids have been reported (Harada 1992) and there are two reports on embryo culture in Limonium (Morgan et al 1995(Morgan et al , 1998.…”

Section: Introductionmentioning

confidence: 99%

Limonium Breeding: New Options for a Well Known Genus

Morgan¹,

Burge²,

Seeley³

2001

Acta Hortic.

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Limonium is a genus that is well known in international cut flower markets. There has been considerable investment in breeding new varieties over many years and the objective has been to try to incorporate traits from less well known species into new cultivars. Limonium perigrinum was hybridized with L. purpuratum and a plant with stems in excess of 60 cm selected from this cross for further development. This plant was later released under PVR as 'Chorus Magenta'. Subsequent breeding has had two aims -to increase the flower color range, but maintain the form of 'Chorus Magenta', and to generate a range of Limonium hybrids for the potted plant market. There are four selections in trials as potted plants in New Zealand and Australia at present. Limonium perezii was hybridized with L. sinuatum using embryo rescue techniques. The hybrid plants were sterile. Use of spindle toxins resulted in a doubling of the nuclear DNA contents of the hybrids and a large proportion of the pollen staining with Alexander's stain. Reciprocal crosses between the DNA tetraploids and their diploid parent species are producing embryos.

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“…In flower crops, the technique of ovule culture or embryo culture is widely applicable for producing new cultivars in interspecific crosses in Lilium (Asano, 1980;Van-Tuyl et al, 1990, 1991, Cyclamen (Ishizaka & Uematsu, 1992), Pelargonium (Bentvelsen, 1990) and Camellia (Yamaguchi et al, 1987). However, this technique has not applied to Gypsophila yet.…”

Section: Introductionmentioning

confidence: 99%

Production of an interspecific hybrid in Gypsophila by ovule-embryo culture

Kishi¹,

Kagami

Shinohara

et al. 1993

Euphytica

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We have succeeded in producing useful interspecific hybrid using ovule-embryo culture between Gypsophila paniculata L. 'Red Sea' and G. manginii, an incompatible combination by ordinary cross breeding methods. The hybrid plant had double flowers with a color of pale purplish pink. Hybrid characteristics of the plant were firmed by observation of plant form, flower type, chromosome number and peroxidase isozyme patterns.

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Overcoming Interspecific Crossing Barriers in Lilium by Ovary and Embryo Culture

Cited by 17 publications

References 0 publications

Distant Hybrids and Their Verification by Means of Rapd

Distant Hybrids and Their Verification by Means of Rapd

Limonium Breeding: New Options for a Well Known Genus

Production of an interspecific hybrid in Gypsophila by ovule-embryo culture

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