Overcoming Incompatibility in Brassica campestris L. by Carbon Dioxide, and Dark Fixation of the Gas by Self- and Cross-pollinated Pistils

Dhaliwal, A. S.; Malik, C. P.; Singh, Manpreet

doi:10.1093/oxfordjournals.aob.a086117

Cited by 22 publications

(10 citation statements)

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“…In this species, the incompatibility reaction appeared to be blocked at both low and high temperatures. Recent experiments have shown that other environmental factors, for example, concentration of atmospheric carbon dioxide, may change the expression of sporophytic self-incompatibility in Brassica (DHALIWAL et al 1981). Both changes in temperature, and high concentrations of carbon dioxide may alter the recognition mechanism, and perhaps negate the onset of inhibition.…”

Section: Pollen Germination and Tube Growthmentioning

confidence: 99%

Pollen-Pistil Interactions

Knox¹

1984

Cellular Interactions

186

134

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Section: Pollen Germination and Tube Growthmentioning

confidence: 99%

Pollen-Pistil Interactions

Knox¹

1984

Cellular Interactions

186

134

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“…Previously, adding carbon dioxide to the environment has been shown to induce seed set in SI Brassica genotypes (O'Neill et al, 1988). Carbon dioxide has been suggested to overcome SI by enhancing pollen activity during germination and tube growth (Dhaliwal et al, 1981), blocking the callose response in the stigmatic papilla cell in expression of SI (O'Neill et al, 1984), and increasing the rate of pollen adhesion (Palloix et al, 1985). Of the 1000 M 1 lines incubated, we successfully recovered and grew 255 individual M 2 lines for use in AtSI mutant screening (Fig.…”

Section: Resultsmentioning

confidence: 94%

Isolation and Characterization of an Arabidopsis Thaliana Self-Incompatibility Mutant Induced by Heavy-Ion Beam Irradiation

Lai¹,

Takehisa²

2013

Acta Biologica Cracoviensia Series Botanica

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Self-incompatibility (SI) is a genetic system that promotes outcrossing by rejecting self-pollen. In the Brassicaceae the SI response is mediated by the pistil S-locus receptor kinase (SRK) and its ligand, pollen Slocus cysteine-rich (SCR) protein. Transfer of SRK-SCR gene pairs to self-fertile Arabidopsis thaliana enabled establishment of robust SI, making this transgenic self-incompatible A. thaliana an excellent platform for SI analysis. Here we report isolation of a novel A. thaliana self-incompatibility mutant, AtC24 SI mutant, induced by heavy-ion beam irradiation. We show that the AtC24 SI mutant exhibits breakdown of SI, with pollen hydration, pollen tube growth and seed set resembling the corresponding processes in wild-type (self-fertile) A. thaliana. Further reciprocal crosses indicated that some perturbed SI factor in the stigmatic cell of the AtC24 SI mutant is responsible for the observed phenotype, while the pollen response remained intact. Our results demonstrate successful application of heavy-ion beam irradiation to induce a novel A. thaliana self-incompatibility mutant useful for SI studies.K Ke ey y w wo or rd ds s: : Arabidopsis thaliana accession C24, heavy-ion beam, pollen, pollen tube, self-incompatibility.

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“…1). In B. campestris, CO2 has been shown to promote both pollen germination and elongation of the pollen tube on the pollinated stigma (Dhaliwal et al 1981). They also state that these stimulative effects were accompanied by increased phosphoenol pyruvate (PEP) carboxylase activity in the pollen.…”

Section: Discussionmentioning

confidence: 99%

“…The role of CO2 in the blocking of self-incompatibility includes: (1) the enhancement of pollen activity during germination and tube growth with an increasing rate of phosphoenol pyruvate carboxylase activity (Dhaliwal et al 1981); (2) blocking of the callose response into the stigmatic papilla cell in the expression of self-incompatibility (O'Neill et al 1984);and (3) an increase in the rate of pollen adhesion, which is an initial event of the pollen-stigma interaction (Palloix et al 1985). Thus, a knowledge of the physiological basis of self-incompatibility is needed in order to understand the role played by CO2 in overcoming selfincompatibility.…”

Section: Introductionmentioning

confidence: 99%

Changes in pollen tube behaviour induced by carbon dioxide and their role in overcoming self-incompatibility in Brassica

Nakanishi

Sawano

1989

Sexual Plant Reprod

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Comparative studies on self-pollination after the application of high concentrations of CO2 gas, which is known to overcome the self-incompatibility reaction in Cruciferous species, have revealed significant changes in the pollen tubes of germinating grains prior to their penetration into the papilla cells. A remarkable increase in the width of the pollen tube was induced by treating the stigmatic papillae with high CO2 concentrations. The width of the pollen tube appeared to be greatest with CO2 concentrations ranging from 3% to 5% ; these concentrations were also optimal for tube penetration. Callose accumulation was extensively induced in the stigmatic papilla with 10%-20% of CO2, although a typical callosic reaction remained through the ranges appropriate for blocking self-incompatibility. Observations using the scanning electron microscope (SEM) after pollination revealed that compatible pollen tubes in cross-pollinations fused completely to the papillar surface during tube penetration, while in self-pollination, pollen tubes remained on the papilla with some additional diffusate. In the case of CO2 treatment for self-pollination, some pollen tubes behaved very similarly to the incompatible or compatible ones already described, while others were different from both of them: they showed a complete fusion, similar to compatible ones, with additional diffusate, similar to incompatible ones. These responses of the pollen and stigma to high CO2 concentrations are discussed with respect to their effect upon the expression of self-incompatibility.

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Overcoming Incompatibility in Brassica campestris L. by Carbon Dioxide, and Dark Fixation of the Gas by Self- and Cross-pollinated Pistils

Cited by 22 publications

References 0 publications

Pollen-Pistil Interactions

Pollen-Pistil Interactions

Isolation and Characterization of an Arabidopsis Thaliana Self-Incompatibility Mutant Induced by Heavy-Ion Beam Irradiation

Changes in pollen tube behaviour induced by carbon dioxide and their role in overcoming self-incompatibility in Brassica

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