Overcoming hybrid lethality in a cross between Japanese pear and apple using gamma irradiation and confirmation of hybrid status using flow cytometry and SSR markers

Gonai, Takeru; Manabe, Toru; Inoue, Eiichi; Hayashi, Mikio; Yamamoto, Takatsugu; Hayashi, Tateki; Sakuma, Fumio; Kasumi, Masakazu

doi:10.1016/j.scienta.2006.02.026

Cited by 15 publications

(6 citation statements)

References 26 publications

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“…Two main in vitro techniques, embryo rescue and protoplast fusion, are used to cross species that otherwise cannot be successfully crossed. Gonai et al (2006), after pollinating pistils of Pyrus pyrifolia with pollen of Malus domestica, cultured the fertilized embryos in vitro and obtained intergeneric shoots with a DNA content intermediate between the parents. Embryo rescue was also used to obtain hybrids between different Cucurbita species, and in all of them the relative nuclear DNA content confirmed successful hybridization (Šiško et al, 2003).…”

Section: In Vitro Culturesmentioning

confidence: 99%

Flow cytometry – a modern method for exploring genome size and nuclear DNA synthesis in horticultural and medicinal plant species

Śliwińska

2018

Folia Horticulturae

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Flow cytometry (FCM) has been used for plant DNA content estimation since the 1980s; however, presently, the number of laboratories equipped with flow cytometers has significantly increased and these are used extensively not only for research but also in plant breeding (especially polyploid and hybrid breeding) and seed production and technology to establish seed maturity, quality and advancement of germination. A broad spectrum of horticultural and medicinal species has been analyzed using this technique, and various FCM applications are presented in the present review. The most common application is genome size and ploidy estimation, but FCM is also very convenient for establishing cell cycle activity and endoreduplication intensity in different plant organs and tissues. It can be used to analyze plant material grown in a greenhouse/field as well asin vitro. Due to somaclonal variation, plant material grown in tissue culture is especially unstable in its DNA content and, therefore, FCM analysis is strongly recommended. Horticultural species are often used as internal standards in genome size estimation and as models for cytometrically studied cytotoxic/anticancer/allelopathic effects of different compounds. With the growing interest in genome modification, increased application of FCM is foreseen.

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Section: In Vitro Culturesmentioning

confidence: 99%

Flow cytometry – a modern method for exploring genome size and nuclear DNA synthesis in horticultural and medicinal plant species

Śliwińska

2018

Folia Horticulturae

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“…Our intergeneric crosses in both directions, contribute an important and unique set of germplasm to researchers and breeders. No successful pear x apple crosses had been raised to fruiting previously [ 16 ]. The previously characterized intergeneric apple-pear hybrid Zwintzscher’s Hybrid served as a positive control for the validation of the methods we employed to characterize our new hybrids.…”

Section: Discussionmentioning

confidence: 99%

“…In a study of hybrids between Japanese pear and apple, where hybrid embryos were gamma-irradiated and cultured at normal temperatures, to obtain viable intergeneric offspring, Gonai et al . [ 16 ] reported that embryos were mostly aborted at an early developmental stage, or seedlings died within six months. In 2014, Zwintzscher’s Hybrid, which resulted from a cross between Malus and Pyrus by Max Zwintscher was reported to be intergeneric [ 12 ], using S-allele and DNA content, as well as metabolomics analyses.…”

Section: Introductionmentioning

confidence: 99%

Molecular characterization of intergeneric hybrids between Malus and Pyrus

Pasqualetto

Palmieri

Martens

et al. 2022

Horticulture Research

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Apple (Malus) and pear (Pyrus) are economically important fruit crops well known for their unique textures, flavours, and nutritional qualities. Both genera are characterised by a distinct pattern of secondary metabolites, which directly affect not only resistance to certain diseases, but also have significant impacts on the flavour and nutritional value of the fruit. The identical chromosome numbers, similar genome size, and their recent divergence date, together with DNA markers have shown that apple and pear genomes are highly co-linear. This study utilized comparative genomic approaches, including simple sequence repeats, high resolution single nucleotide polymorphism melting analysis, and single nucleotide polymorphism chip analysis to identify genetic differences among hybrids of Malus and Pyrus, and F2 offspring. This research has demonstrated and validated that these three marker types, along with metabolomics analysis are very powerful tools to detect and confirm hybridity of progeny derived from crosses between apple and pear in both cross directions. Furthermore, this work analysed the genus-specific metabolite patterns and the resistance to fire blight (Erwinia amylovora) in progeny. The findings of this work will enhance and accelerate the breeding of novel tree fruit crops that benefit producers and consumers, by enabling marker assisted selection of desired traits introgressed between pear and apple.

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“…Analysis of relative nuclear DNA content using flow cytometry is simple and has been used to determine many hybrids (Amano et al 2007;Chen and Mii 2012;Gonai et al 2006;Mahoney and Brand 2021;Oku et al 2008). In the flow cytometry analysis of Argyranthemum, the peak position of the nuclear DNA content of A. frutescens overlapped with that of G. coronaria, and the hybridity could not be determined; however, in the cross with I. carinata, the peak position was different and the determination of hybridity was possible (Ueda and Yamada 2005).…”

Section: Discussionmentioning

confidence: 99%

Intergeneric hybridization of marguerite (<i>Argyranthemum frutescens</i> (L.) Sch. Bip.) and Roman chamomile (<i>Chamaemelum nobile</i> (L.) All.) using ovule culture and confirmation of hybridity

Katsuoka

Hamabe

Kato

et al. 2022

Plant Biotechnology

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To introduce useful characteristics such as fragrance into Argyranthemum frutescens (L.) and to expand the variation, we conducted crosses using A. frutescens as the seed parent and Chamaemelum nobile (L.) All. as the pollen parent. All the tested cross combinations between the three strains of A. frutescens and one strain of C. nobile produced embryos, and healthy plants were obtained by ovule culture. The obtained plantlets had a white ray floret, and the leaf shape was intermediate to those of the parents. The individuals obtained from this cross were subjected to two methods to determine hybridity: flow cytometry analyses and cleaved amplified polymorphic sequence (CAPS) markers. For the CAPS marker, we selected the internal transcribed spacer (ITS) region, which is highly variable among the genera, as the region to be amplified. We selected restriction enzymes BmgT120 I and Afl II, which selectively cut common sequences in the genus Argyranthemum, based on the sequence analysis of one parent strain each of A. frutescens and C. nobile and alignment with known sequences of related species. Flow cytometry analyses and CAPS markers revealed that the individuals obtained from the cross between A. frutescens and C. nobile are intergeneric hybrids. In addition, these established methods were capable of quickly and reliably identifying hybrids between A. frutescens and C. nobile. This report shows for the first time that crossbreeding between A. frutescens (seed parent) and C. nobile (pollen parent) is possible, and further development of Argyranthemum breeding, such as the expansion of variation by intergeneric crosses, is expected.

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Overcoming hybrid lethality in a cross between Japanese pear and apple using gamma irradiation and confirmation of hybrid status using flow cytometry and SSR markers

Cited by 15 publications

References 26 publications

Flow cytometry – a modern method for exploring genome size and nuclear DNA synthesis in horticultural and medicinal plant species

Flow cytometry – a modern method for exploring genome size and nuclear DNA synthesis in horticultural and medicinal plant species

Molecular characterization of intergeneric hybrids between Malus and Pyrus

Intergeneric hybridization of marguerite (<i>Argyranthemum frutescens</i> (L.) Sch. Bip.) and Roman chamomile (<i>Chamaemelum nobile</i> (L.) All.) using ovule culture and confirmation of hybridity

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