Overcoming functional challenges in autologous and engineered fat grafting trends

Major, Gretel; Simcock, Jeremy; Woodfield, Tim B. F.; Lim, Khoon S.

doi:10.1016/j.tibtech.2021.04.006

Cited by 16 publications

(16 citation statements)

References 98 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…[87] However, inconsistent vascularization and unpredictable survival volume place limitations on the repair size. [88] In 2006, Matsumoto et al first reported a technique named cell-assisted lipotransfer (CAL). [89] They combined aspirated fat with ADSCs to create stem cell-rich fat grafts and transplanted them subcutaneously into mice with severe combined immunodeficiency.…”

Section: Adscs For Fat Graftingmentioning

confidence: 99%

See 1 more Smart Citation

An Update on Adipose‐Derived Stem Cells for Regenerative Medicine: Where Challenge Meets Opportunity

Qin

Yang

et al. 2023

Advanced Science

View full text Add to dashboard Cite

Over the last decade, adipose‐derived stem cells (ADSCs) have attracted increasing attention in the field of regenerative medicine. ADSCs appear to be the most advantageous cell type for regenerative therapies owing to their easy accessibility, multipotency, and active paracrine activity. This review highlights current challenges in translating ADSC‐based therapies into clinical settings and discusses novel strategies to overcome the limitations of ADSCs. To further establish ADSC‐based therapies as an emerging platform for regenerative medicine, this review also provides an update on the advancements in this field, including fat grafting, wound healing, bone regeneration, skeletal muscle repair, tendon reconstruction, cartilage regeneration, cardiac repair, and nerve regeneration. ADSC‐based therapies are expected to be more tissue‐specific and increasingly important in regenerative medicine.

show abstract

Section: Adscs For Fat Graftingmentioning

confidence: 99%

“…[ 87 ] However, inconsistent vascularization and unpredictable survival volume place limitations on the repair size. [ 88 ] In 2006, Matsumoto et al. first reported a technique named cell‐assisted lipotransfer (CAL).…”

Section: Adscs In Regenerative Therapies: An Overview Of Recent Advan...mentioning

confidence: 99%

An Update on Adipose‐Derived Stem Cells for Regenerative Medicine: Where Challenge Meets Opportunity

Qin

Yang

et al. 2023

Advanced Science

View full text Add to dashboard Cite

show abstract

“…Currently, fat grafting is an effective strategy for reconstructing congenital and acquired soft tissue defects caused by sequelae of mastectomy, radiation therapy, trauma, and facial rejuvenation. Notably, the survival rate of transplanted fat tissue ranges from 20 to 80%, which is mainly due to delayed and insufficient re-vascularization [ 2 , 11 ]. Angiogenesis is the process by which new vessels form.…”

Section: Introductionmentioning

confidence: 99%

High yield engineered nanovesicles from ADSC with enriched miR-21-5p promote angiogenesis in adipose tissue regeneration

et al. 2022

View full text Add to dashboard Cite

Background Mesenchymal stem cell-derived extracellular vesicles (MSC-EVs) have been found to have a great potential for soft tissue repair due to various biological functions, including pro-angiogenesis and low immunogenicity. However, the low yield and heterogeneity of MSC-EVs limited their clinical transformation. This study was designed to develop a novel adipose-derived stem cell engineered nanovesicles (ADSC-NVs) with high production and explore its pro-angiogenetic effect and application in adipose tissue regeneration. Methods Adipose-derived stem cell-derived extracellular vesicles (ADSC-EVs) were isolated from an EVs-free culture medium for human ADSCs (hADSCs). ADSC-NVs were prepared by sequentially extruding ADSCs followed by iodixanol density gradient ultracentrifugation and were compared with ADSC-EVs in morphology, size distribution, protein contents and yield. The pro-angiogenetic effect of ADSC-NVs in different doses (0, 5, 20 and 80 μg/mL) in vitro was determined using transwell assay, tube formation assay, western blot and qRT-PCR. In vivo, BALB/c nude mice were administered injection of a mixture of fat granules and different dose of ADSC-NVs and grafts were harvested at 12 weeks post-transplantation for further analysis. By analyzing the weight and volume of grafts and histological evaluation, we investigated the effect of ADSC-NVs in vessel formation and adipose tissue regeneration. Results Our results showed yield of purified ADSC-NVs was approximately 20 times more than that of ADSC-EVs secreted by the same number of ADSCs. In vitro, both ADSC-NVs and ADSC-EVs exhibited a dose-dependent pro-angiogenetic effect, despite their distinct miRNA profiles. These effects of ADSC-NVs may be mediated by enriched miR-21-5p via PTEN inhibition and PI3K/p-Akt signaling activation. Furthermore, after a mixed injection of ADSC-NVs, vessel formation and adipose regeneration were observed in vivo in fat implants. Conclusions Our study developed a potent alternative of ADSC-EVs. ADSC-NVs have a high pro-angiogenesis potential and can be used as cell-free therapeutic biomaterials in soft tissue regeneration.

show abstract

“…1 Besides, the TEC works as a bona fide bioreactor promoting adipogenesis via several complementary mechanisms. 2 Thus, in many preclinical models, TEC promotes significant long-term growth (up to fivefold the initial volume) for fat flaps. [3][4][5] Furthermore, its clinical feasibility was validated in women with history of postmastectomy reconstruction.…”

Section: Introductionmentioning

confidence: 99%

Preclinical Study of Radiation on Fat Flap Regeneration under Tissue-engineering Chamber: Potential Consequences for Breast Reconstruction

Cleret¹,

Gradwohl

Dekerle

et al. 2022

Plastic and Reconstructive Surgery - Global Open

View full text Add to dashboard Cite

Background: Use of a tissue-engineering chamber (TEC) for growth of fat flap is a promising approach for breast reconstruction. Here, we evaluated in a preclinical model the effects of radiation on adipose tissue growth either before or after 3D-printed bioresorbable TEC implantation. Methods: Twenty-eight female Wistar rats were distributed into three groups: TEC implantation as nonirradiated controls (G1), TEC insertion followed by irradiation 3 weeks later (G2), and irradiation 6 weeks before TEC insertion (G3). G2 and G3 received 33.3 Gy in nine sessions of 3.7 Gy. Growth of the fat flap was monitored via magnetic resonance imaging. At 6 months after implantation, fat flaps and TECs were harvested for analysis. Results: Irradiation did not alter the physicochemical features of poly(lactic-coglycolic acid)-based TECs. Compared with G1, fat flap growth was significantly reduced by 1.6 times in irradiated G2 and G3 conditions. In G2 and G3, fat flaps consisted of mature viable adipocytes sustained by CD31+ vascular cells. However, 37% (3 of 8) of the G2 irradiated adipose tissues presented a disorganized architecture invaded by connective tissues with inflammatory CD68 + cells, and the presence of fibrosis was observed. Conclusions: Overall, this preclinical study does not reveal any major obstacle to the use of TEC in a radiotherapy context. Although irradiation reduces the growth of fat flap under the TEC by reducing adipogenesis and inducing inconsistent fibrosis, it does not impact flap survival and vascularization. These elements must be taken into account if radiotherapy is proposed before or after TEC-based breast reconstruction.

show abstract

Overcoming functional challenges in autologous and engineered fat grafting trends

Cited by 16 publications

References 98 publications

An Update on Adipose‐Derived Stem Cells for Regenerative Medicine: Where Challenge Meets Opportunity

An Update on Adipose‐Derived Stem Cells for Regenerative Medicine: Where Challenge Meets Opportunity

High yield engineered nanovesicles from ADSC with enriched miR-21-5p promote angiogenesis in adipose tissue regeneration

Preclinical Study of Radiation on Fat Flap Regeneration under Tissue-engineering Chamber: Potential Consequences for Breast Reconstruction

Contact Info

Product

Resources

About