Overcoming extended lag phase on optically pure lactic acid production from pretreated softwood solids

Campos, Joana; Almqvist, Henrik; Bao, Jie; Wallberg, Ola; Lidén, Gunnar

doi:10.3389/fbioe.2023.1248441

Cited by 3 publications

(1 citation statement)

References 46 publications

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“…can manufacture L (+) lactic acid from starch, but the yield is very low as compared to LAB ( Ghaffar et al, 2014 ). Optically pure LA can be effectively synthesized by commercial large-scale microbial fermentations without the need for downstream processing to separate the two enantiomers, namely, L(+)-lactic acid and D(−)-lactic acid ( Campos et al, 2023 ).…”

Section: Introductionmentioning

confidence: 99%

Homofermentative Lactobacilli isolated from organic sources exhibit potential ability of lactic acid production

Stephen,

Saleh

2023

Front. Microbiol.

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There has been an increasing interest in recent years in lactic acid bacteria that are derived from organic sources for lactic acid production. This research article presents the isolation and identification of homofermentative lactic acid bacteria from various novel organic sources, followed by qualitative and quantitative analyses of lactic acid produced. A total of 32 isolates were identified initially from various sources, such as curd (C1, C2), probiotics (P1, P2, and P3), silage (Si1 and Si2), soil samples (S1, S2, and S3), vermicompost (V1 and V2), and Farmyard manure. Biochemical tests such as Gram’s staining, catalase test, and oxidase test were conducted for preliminary identification of lactic acid bacteria using De Man, Rogosa, and Sharpe agar (MRS) media. Through selection and identification, based on colony morphology and biochemical characteristics, 18 isolates were identified as lactic acid bacteria. The subsequent analysis included a tube test, screening for organic acid production, and homofermentative screening using homofermentative–heterofermentative differential (HHD) medium for qualitative analysis of lactic acid. The results revealed that 9 out of 18 selected strains were homofermentative and had promising potential for the production of lactic acid. Furthermore, six isolates (P1-1, S1-3, C2-1, V2-3, P2-1, and C1-1) from all of the nine positive strains were subjected to pH testing (0, 24, 48, and 72 h) and titrimetric assay for estimation of % crude lactic acid present. The presence of lactic acid was confirmed using thin-layer chromatography (TLC). L (+)-Lactic acid was quantified using a K-LATE enzymatic assay kit, for the best three isolates (P1-1, S1-3, C2-1). Finally, the strains were subjected to 16SrRNA sequencing and were identified as Lactobacilli. Based on the findings of the study, it could be concluded that homofermentative lactic acid bacteria with significant LA-producing ability can be obtained from different organic sources and may prove to be useful in the successful production of lactic acid for biotechnological applications.

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Section: Introductionmentioning

confidence: 99%

Homofermentative Lactobacilli isolated from organic sources exhibit potential ability of lactic acid production

Stephen,

Saleh

2023

Front. Microbiol.

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Waste Viscose for Optically pure Lactic acid Production

Campos,

Bågenholm-Ruuth,

Sanchis-Sebastiá

et al. 2024

Waste Biomass Valor

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Recycling of textile fibers is a mandatory step in the life-cycle of fabrics to avoid incineration or landfilling. After fiber-to-fiber recycling, cellulose fiber saccharification opens the door to the production of biochemical building blocks through microbial fermentation. This study documents for the first time the production of optically pure D-lactic acid (D-LA), one of the precursors of polylactic acid (PLA), from post-consumer waste viscose. Enzymatic hydrolysis was applied to white and colored waste viscose to produce two glucose-rich solutions that were used as carbon source for the genetically modified Pediococcus acidilactici ZP26. Afterward, batch and fed-batch cultivations were conducted with very similar results for the control and both waste viscose hydrolysates (white and colored). The strain was able to produce D-LA without any signs of inhibition by possibly toxic compounds present in the recycled textiles (additives, dyes, and other contaminants). The highest D-LA concentration and yield was reached in the batch reactors with colored waste viscose at 42.4 g/L and 0.97 g/g, respectively. In the fed-batch cultivations, the best result was achieved with the white waste solution, with 59.5 g of D-LA (40.8 g/L), and a yield on total glucose of 0.70 g/g. Notwithstanding the need for more research in the utilization of saccharified waste viscose as carbon source for bioprocesses, this is an important proof of concept of an application of streams from tertiary recycling of cellulosic fibers in the production of platform biochemicals. Graphical Abstract

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Antimicrobial effects and metabolomics analysis of cell-free supernatant produced by Pediococcus acidilactici LWX 401 isolated from Yunnan traditional pickles

Liu,

Wang,

Xiao

et al. 2024

LWT

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Overcoming extended lag phase on optically pure lactic acid production from pretreated softwood solids

Cited by 3 publications

References 46 publications

Homofermentative Lactobacilli isolated from organic sources exhibit potential ability of lactic acid production

Homofermentative Lactobacilli isolated from organic sources exhibit potential ability of lactic acid production

Waste Viscose for Optically pure Lactic acid Production

Antimicrobial effects and metabolomics analysis of cell-free supernatant produced by Pediococcus acidilactici LWX 401 isolated from Yunnan traditional pickles

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