Overall Prevalence and Distribution of Knockdown Resistance (kdr) Mutations in Aedes aegypti from Mandalay Region, Myanmar

Naw, Haung; Su, Mya Nilar Chaw; Võ, Tuấn Cường; Lê, Hương Giang; Kang, Jung‐Mi; Jun, Hojong; Mya, Yi Yi; Myint, Moe Kyaw; Lee, Jin Young; Sohn, Woon-Mok; Kim, Tong‐Soo; Na, Byoung-Kuk

doi:10.3347/kjp.2020.58.6.709

Cited by 3 publications

(12 citation statements)

References 30 publications

(40 reference statements)

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“…In our previous study, we analyzed the overall profiles of kdr mutations in vgsc of Ae. aegypti collected from the Mandalay area of Myanmar and found substantial levels of potent insecticide resistance in the population [21]. However, our previous study was a pooled analysis of Ae.…”

Section: Discussionmentioning

confidence: 82%

“…High frequencies of these mutations were also reported in our previous study using pooled Ae. aegypti samples collected from the Mandalay area in 2017, but with slightly different frequencies of S989P (68.6%), V1016G (73.5%), and F1534C (40.1%) [21]. High levels of S989P (78.8%), V1016G (84.4%), and F1534C (23.2%) were also reported in the Ae.…”

Section: Discussionmentioning

confidence: 95%

“…Among these mutations, S989P, V1016G, and F1534C are the most common mutations found in Asian Ae. aegypti populations [15,19,21,[24][25][26][27][28]. In our previous study, we analyzed the overall profiles of kdr mutations in vgsc of Ae.…”

Section: Discussionmentioning

confidence: 99%

“…aegypti mosquitoes individually using a Tissue Genomic DNA Extraction Kit (Bioneer, Daejeon, Korea) following the manufacturer's instructions. Fragments of the vgsc flanking segment 6 of domain II (DII-S6) and segment 6 of domain III (DIII-S6) were amplified via polymerase chain reaction (PCR) with specific primer sets: AaNavF20 (5 -ACAATGTGGATCGCTTCCC-3 ) and AaNavR21 (5 -TGGACAAAAGCAAGGCTAAG-3 ) for DII-S6, and AaNavEx31P (5 -TCGCGGGAG-GTAAGTTATTG-3 ) and AaNavEx31Q (5 -GTTGATGTGCGATGGAAATG-3 ) for DIII-S6 [21,23]. The thermal cycling conditions were initial denaturing at 95 • C for 10 min, followed by 35 cycles of denaturation at 95 • C for 30 s, annealing at 56 • C for 30 s and extension at 72 • C for 30 s, and the final extension at 72 • C for 5 min.…”

Section: Dna Extraction and Gene Amplificationmentioning

confidence: 99%

“…Molecular studies revealed a substantial number of kdr mutations associated with insecticide resistance in the Myanmar Ae. aegypti population [19][20][21]. In this study, we analyzed the patterns and prevalence of kdr mutations in vgsc of Ae.…”

Section: Introductionmentioning

confidence: 99%

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Knockdown Resistance Mutations in the Voltage-Gated Sodium Channel of Aedes aegypti (Diptera: Culicidae) in Myanmar

Naw

Võ

Lê

et al. 2022

Insects

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Aedes aegypti is an important mosquito vector transmitting diverse arboviral diseases in Myanmar. Pyrethroid insecticides have been widely used in Myanmar as the key mosquito control measure, but the efforts are constrained by increasing resistance. Knockdown resistance (kdr) mutations in the voltage-gated sodium channel (VGSC) are related to pyrethroid resistance in Ae. aegypti. We analyzed the patterns and distributions of the kdr mutations in Ae. aegypti in the Mandalay area of Myanmar. The segment 6 regions of domains II and III of vgsc were separately amplified from individual Ae. aegypti genomic DNA via polymerase chain reaction. The amplified gene fragments were sequenced. High proportions of three major kdr mutations, including S989P (54.8%), V1016G (73.6%), and F1534C (69.5%), were detected in the vgsc of Ae. aegypti from all studied areas. Other kdr mutations, T1520I and F1534L, were also found. These kdr mutations represent 11 distinct haplotypes of the vgsc population. The S989P/V1016G/F1534C was the most prevalent, followed by S989P/V1016V and V1016G/F1534C. A quadruple mutation, S989P/V1016G/T1520I/F1534C, was also identified. High frequencies of concurrent kdr mutations were observed in vgsc of Myanmar Ae. aegypti, suggesting a high level of pyrethroid resistance in the population. These findings underscore the need for an effective vector control program in Myanmar.

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Section: Discussionmentioning

confidence: 82%

Section: Discussionmentioning

confidence: 95%

Section: Discussionmentioning

confidence: 99%

Section: Dna Extraction and Gene Amplificationmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Knockdown Resistance Mutations in the Voltage-Gated Sodium Channel of Aedes aegypti (Diptera: Culicidae) in Myanmar

Naw

Võ

Lê

et al. 2022

Insects

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Detection of pyrethroid resistance mutations and intron variants in the voltage‐gated sodium channel of Aedes (Stegomyia) aegypti and Aedes (Stegomyia) albopictus mosquitoes from Lao People's Democratic Republic

Marcombe

Shimell

Savage

et al. 2022

Medical Vet Entomology

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In Lao People's Democratic Republic, Aedes aegypti (Linnaeus 1762) and Aedes albopictus (Skuse 1894) mosquitoes (Diptera: Culicidae) are vectors of arboviral diseases such as dengue. As the treatment for these diseases is limited, control of the vectors with the use of pyrethroid insecticides is still essential. However, mutations in the voltage‐gated sodium channel (vgsc) gene giving rise to pyrethroid resistance are threatening vector control programs. Here, we analysed both Ae. aegypti and Ae. albopictus mosquitoes, which were collected in different districts of Laos (Kaysone Phomvihane, Vangvieng, Saysettha and Xaythany), for vgsc mutations commonly found throughout Asia (S989P, V1016G and F1534C). Sequences of the vgsc gene showed that the F1534C mutation was prevalent in both Aedes species. S989P and V1016G mutations were detected in Ae. aegypti from each site and were always found together. In addition, the mutation T1520I was seen in Ae. albopictus mosquitoes from Saysettha district as well as in all Ae. aegypti samples. Thus, mutations in the vgsc gene of Ae. aegypti are prevalent in the four districts studied indicating growing insecticide resistance throughout Laos. Constant monitoring programmes and alternative strategies for controlling Aedes should be utilized in order to prolong the effectiveness of pyrethroids thereby maximizing vector control.

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Detection of Putative Mutation I873S in the Sodium Channel of Megalurothrips usitatus (Bagnall) Which May Be Associated with Pyrethroid Resistance

Gao

Qiu

et al. 2023

Insects

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Pyrethroid resistance of thrips has been reported in many countries, and knockdown resistance (kdr) has been identified as a main mechanism against pyrethroids in many insects. To characterize pyrethroid resistance in Megalurothrips usitatus from the Hainan Province of China, we conducted a biological assay and sequenced the voltage-gated sodium channel gene domain II from M. usitatus field populations. It showed high resistance to the pyrethroids for 2019 and 2020, in which LC50 to lambda-cyhalothrin of M. usitatus was 1683.521 mg/L from Sanya in 2020. The LC50 value of deltamethrin was lower in Haikou than in other locations, which mean the south of Hainan has higher resistance than the north of Hainan. Two mutations of I873S and V1015M were detected in the domain II region of the sodium channel in M. usitatus; however, the mutation frequency of V1015M was only 3.33% and that of I873S was 100%. One is homozygous and the other is a heterozygous mutant type. The three thrips-sensitive strains of sodium channel 873 are highly conserved in amino acids (isoleucine), while the M. usitatus pyrethroid-resistant strains are all serine, so I873S may be related to the resistance of M. usitatus to pyrethroids. The present study will contribute to the understanding of the evolution of pyrethroids resistance and contribute to the development of resistance management of M. usitatus in Hainan.

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Overall Prevalence and Distribution of Knockdown Resistance (kdr) Mutations in Aedes aegypti from Mandalay Region, Myanmar

Cited by 3 publications

References 30 publications

Knockdown Resistance Mutations in the Voltage-Gated Sodium Channel of Aedes aegypti (Diptera: Culicidae) in Myanmar

Knockdown Resistance Mutations in the Voltage-Gated Sodium Channel of Aedes aegypti (Diptera: Culicidae) in Myanmar

Detection of pyrethroid resistance mutations and intron variants in the voltage‐gated sodium channel of Aedes (Stegomyia) aegypti and Aedes (Stegomyia) albopictus mosquitoes from Lao People's Democratic Republic

Detection of Putative Mutation I873S in the Sodium Channel of Megalurothrips usitatus (Bagnall) Which May Be Associated with Pyrethroid Resistance

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