Overabundance of Veillonella parvula promotes intestinal inflammation by activating macrophages via LPS-TLR4 pathway

Abstract

Hirschsprung’s disease-associated enterocolitis (HAEC) is the most common complication of Hirschsprung’s disease (HSCR). The microbiome pattern of intestinal flora in HAEC patients was significantly abnormal compared to that in HSCR patients. The overabundance of V. parvula was detected in the gut of HAEC patients. To elucidate the pathological mechanisms of the overabundance of V. parvula, we established and analyzed inflammatory models induced by LPS or single-bacterial strain transplantation in vivo. The transplantation of V. parvula induced inflammatory response in the colon of mice. Besides, we found that LPS from V. parvula can significantly impair the barrier function of colonic epithelial cells and then activate macrophages which impaired pacemaker function of interstitial cells of Cajal (ICCs). It was thus a vicious cycle, where the macrophage-related inflammation caused by V. parvula via LPS-TLR4 pathway damaged the intestinal motility, which further aggravated the intestinal flora dysbiosis and promoted the development of HAEC. Itaconic acid could break the vicious cycle by inhibiting the activation of macrophages. It could be a potential therapeutic strategy for HAEC patients with intestinal flora dysbiosis.

Characteristics of gut microbiota and fecal metabolomes in patients with celiac disease in Northwest China

“…Based on species at genus level, the diagnostic models were constructed to distinguish CD patients from healthy controls. the saliva and tissues of CD patients (Nistal et al, 2012a(Nistal et al, , 2016Iaffaldano et al, 2018;Serena et al, 2019a;Zhan et al, 2022), and similar results were found in the stool samples of this study.…”

Pterostilbene Prevents Tunicamycin-Induced Intestinal Barrier Damage by Targeting Endoplasmic Reticulum Stress, Oxidative Stress, Autophagy, and Gut Microbiota

“…Apart from the increase in microbial diversity, PT also inhibited the abnormal proliferation of several conditioned pathogens in the cecum of the TM-exposed piglets. For instance, Campylobacter and Veillonella are detrimental to intestinal barrier function, and their relative abundance is positively related to the risk of intestinal diseases such as diarrhea. − In addition, Actinobacillus is found to be enriched in the gut of piglets infected with epidemic diarrhea virus with an inhibitory role on intestinal energy and amino acid metabolism . In this study, the relative abundance of Campylobacter , Veillonella , and Actinobacillus was significantly reduced by PT treatment.…”

Structural and functional alterations of intestinal flora in mice induced by halonitromethanes exposure

“…Previous studies have shown that some bacteria among Acinetobacter, are opportunistic pathogens causing gastroenteritis such as Acinetobacter lwoffii and Acinetobacter johnsonii (Palmisano et al, 2020). Meanwhile, Morganella, Proteus, Veillonella, Actinomyces, Prevotella, Clostridium, Granulicatella were significantly enriched in the least one BNM, BCNM, or TCNM exposure group, which have been proved to be closely related to gastroenteritis (Hamilton et al, 2018;Li et al, 2018;Kiu et al, 2019;Bockmeyer et al, 2020;Iljazovic et al, 2021;Tian et al, 2022;Zhan et al, 2022). Accumulating evidence strongly suggests that intestinal dysbacteriosis and enteritis-associated pathogens bloom are important causes of intestinal inflammation, lesions, and even cancer (Gao et al, 2018;Khan et al, 2019;Liu et al, 2021).…”