Over-expression of the cloned rice thaumatin-like protein (PR-5) gene in transgenic rice plants enhances environmental friendly resistance to Rhizoctonia solani causing sheath blight disease

Datta, Karabi; Velazhahan, R.; Oliva, Norman; Oña, I.; Mew, T. W.; Khush, G. S.; Muthukrishnan, Subbaratnam; Datta, Swapan K.

doi:10.1007/s001220051178

Cited by 313 publications

(160 citation statements)

References 32 publications

Supporting

Mentioning

152

Contrasting

Unclassified

Order By: Relevance

“…Some plant TL proteins show endo-b-1,3-glucanase activity (Grenier et al,1999) and help defend against pathogenic fungi (Datta et al, 1999). Nematodes and locusts also have TL proteins (Kitajima and Sato, 1999;Brandazza et al, 2004), and they are also subject to predation and pathogenic infection by nematophagous (Thorn and Barron, 1984) and entomopathogenic fungi (Clarkson and Charnley, 1996), respectively.…”

Section: Discussionmentioning

confidence: 99%

“…When analyzed using SDS-PAGE, plant TL proteins are monomeric soluble proteins of low molecular mass (15-39 kD). Activities attributed to these proteins include a sweet taste, antifreeze activity, and antifungal activity (Yun et al, 1998;Datta et al, 1999). In addition, some TL proteins exhibit both b-1,3-glucan binding (Trudel et al, 1998) and endo-b-1,3-glucanase activities (Grenier et al, 1999).…”

mentioning

confidence: 99%

See 1 more Smart Citation

Lentinula edodes tlg1 Encodes a Thaumatin-Like Protein That Is Involved in Lentinan Degradation and Fruiting Body Senescence

et al. 2006

View full text Add to dashboard Cite

Lentinan is an antitumor product that is purified from fresh Lentinula edodes fruiting bodies. It is a cell wall component, comprising b-1,3-glucan with b-1,6-linked branches, which becomes degraded during postharvest preservation as a result of increased glucanase activity. In this study, we used N-terminal amino acid sequence to isolate tlg1, a gene encoding a thaumatin-like (TL) protein in L. edodes. The cDNA clone was approximately 1.0 kb whereas the genomic sequence was 2.1 kb, and comparison of the two indicated that tlg1 contains 12 introns. The tlg1 gene product (TLG1) was predicted to comprise 240 amino acids, with a molecular mass of 25 kD and isoelectric point value of 3.5. The putative amino acid sequence exhibits approximately 40% identity with plant TL proteins, and a fungal genome database search revealed that these TL proteins are conserved in many fungi including the basidiomycota and ascomycota. Transcription of tlg1 was not detected in vegetative mycelium or young and fresh mushrooms. However, transcription increased following harvest. Western-blot analysis demonstrated a rise in TLG1 levels following harvest and spore diffusion. TLG1 expressed in Escherichia coli and Aspergillus oryzae exhibited b-1,3-glucanase activity and, when purified from the L. edodes fruiting body, demonstrated lentinan degrading activity. Thus, we suggest that TLG1 is involved in lentinan and cell wall degradation during senescence following harvest and spore diffusion.

show abstract

Section: Discussionmentioning

confidence: 99%

mentioning

confidence: 99%

Lentinula edodes tlg1 Encodes a Thaumatin-Like Protein That Is Involved in Lentinan Degradation and Fruiting Body Senescence

et al. 2006

View full text Add to dashboard Cite

show abstract

“…Chitinases are the enzymes that rupture chitin i.e. the primary component of cell walls of several types of fungi and exoskeletons of invertebrates by hydrolyzing the β-1,4-linkage among N-acetylglucosamine units (Datta et al, 1999).…”

Section: Introductionmentioning

confidence: 99%

IN VITRO REGENERATION AND TRANSFORMATION OF APPLE (Malus domestica Borkh.) ROOTSTOCK MALLING 7 USING RICE CHITINASE GENE

Sharma¹,

Modgil²,

Sharma³

2017

JEBAS

View full text Add to dashboard Cite

In most of the fruit crops, the low frequency of Agrobacterium mediated genetic transformation hampers the respective transgenics production. Hence, it is pre-requisite to understand and optimize the different parameters involved directly or indirectly in Agrobacterium mediated transformation. A. tumefaciens strain LBA4404 harboring the transforming vector pCAMBARchi11 containing the rice chitinase gene (chi11) and hygromycin phosphotransferase (hpt) and phosphinothricin acetyl transferase (bar) genes was used for carrying out genetic transformation of apple (Malus x domestica Borkh.) rootstock M7. Two explants viz. leaf and internodal segments were involved in regeneration and resulted 24.37% and 60.58% shoot regeneration, respectively. The obtained putative transformants were selected on full strength MS medium containing 5 mg l -1 hygromycin and 500 mg l -1 cefotaxime. Two days pre-culturing and 96 hours co-cultivation was found effective for procuring maximum number of hygromycin resistant shoots. Putative transgenic shoots were multiplied separately and rooted on root induction medium containing 5 mg l -1 hygromycin. Further, PCR analysis was done using chitinase gene specific primers and two apple transgenic lines confirmed the integration of chi11 gene by yielding 237 bp and 584 bp amplified products, respectively.

show abstract

“…Cultural practices and biological and chemical controls have been developed to ameliorate the problem (Nagarajkumar et al, 2004;Rodrigues et al, 2003;Slaton et al, 2003). Several breeding techniques, such as variety screening and transgenic biotechnologies, have been introduced to obtain resistant varieties (Datta et al, 1999;Jia et al, 2006).…”

Section: Introductionmentioning

confidence: 99%

Metabolite profiling of sheath blight disease resistance in rice: in the case of positive ion mode analysis by CE/TOF-MS

Suharti

Nose

Zheng

2016

Plant Production Science

View full text Add to dashboard Cite

Rice sheath blight is an important disease caused by Rhizoctonia solani. The resistant and susceptible rice lines (32R and 29S, respectively) showed different responses to R. solani infection in metabolite levels. The aim of this study was to characterize the metabolite levels in rice lines during R. solani infection using capillary electrophoresis equipped with time of flight mass spectrophotometry (CE/TOF-MS) in positive ion mode. Hundred metabolites were identified and classified into six clusters by hierarchical cluster using Mass Profiler Professional software. Changes in metabolite level at inoculated 32R and 29S were mapped on branches of tricarboxylic acid and glycolysis pathway. Volcano plot successfully filtered the metabolites based on fold change and p-value. The volcano plot result showed that 10 metabolites were up and down regulated in inoculated 32R relative to 29S. One metabolite, chlorogenic acid, showed a positive response in 32R. Meanwhile, pipecolic acid showed as the highest magnitude of fold change and p-value significance level in 29S. In addition, eight amino acids; glutamate, γ-aminobutyric acid, glycine, histidine, phenylalanine, serine, tryptophan, and tyrosine showed increase in 29S after R. solani inoculation.

show abstract

Over-expression of the cloned rice thaumatin-like protein (PR-5) gene in transgenic rice plants enhances environmental friendly resistance to Rhizoctonia solani causing sheath blight disease

Cited by 313 publications

References 32 publications

Lentinula edodes tlg1 Encodes a Thaumatin-Like Protein That Is Involved in Lentinan Degradation and Fruiting Body Senescence

Lentinula edodes tlg1 Encodes a Thaumatin-Like Protein That Is Involved in Lentinan Degradation and Fruiting Body Senescence

IN VITRO REGENERATION AND TRANSFORMATION OF APPLE (Malus domestica Borkh.) ROOTSTOCK MALLING 7 USING RICE CHITINASE GENE

Metabolite profiling of sheath blight disease resistance in rice: in the case of positive ion mode analysis by CE/TOF-MS

Contact Info

Product

Resources

About