Over-Expression of the Apple Gene Mpnpr1 Causes Increased Disease Resistance in Malus X Domestica

Malnoy, Mickaël; Borejsza-Wysocka, Ewa; Jin, Qingxu; He, Sheng Yang; Aldwinckle, Herb S.

doi:10.17660/actahortic.2004.663.80

Cited by 35 publications

(67 citation statements)

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“…Similar limitations were observed in the AtNPR1 transformants against Verticillium wilt, where protection was observed only against the non-defoliating isolates of V. dahliae and not the defoliating isolates (Parkhi et al 2010b). Nevertheless, there are a large number of reports that show that NPR1 overexpression confers a strong protection against a variety of diseases (Lin et al 2004;Makandar et al 2006;Malnoy et al 2007;Wally et al 2009;Zhang et al 2010;Dutt et al 2015;Silva et al 2015). Thus, controlled expression of NPR1 gene may still prove to be a useful means to confer tolerance to some pathogens in the field.…”

Section: Discussionmentioning

confidence: 58%

“…They reported that this resistance against FHB is associated with faster activation of defense response in AtNPR1-expressing transgenic wheat. In another study, Malnoy et al (2007) overexpressed a homologous NPR1 (MpNPR1) in apple. They showed that the transgenic apple plants exhibited increased resistance against apple scab, cedar apple rust and fire blight diseases.…”

Section: Introductionmentioning

confidence: 99%

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Response of AtNPR1-expressing cotton plants to Fusarium oxysporum f. sp. vasinfectum isolates

Joshi

Kumar

Janga

et al. 2017

Physiol Mol Biol Plants

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In our earlier investigation, we had demonstrated that transgenic cotton plants expressing AtNPR1 showed significant tolerance to Fusarium oxysporum f. sp. vasinfectum, isolate 11 (Fov11) and several other pathogens. The current study was designed to further characterize the nature of the protection provided by AtNPR1 expression and its limitations. Green Fluorescent Protein-expressing Fov11 was generated and used to study the progression of the disease within the plant. The results show that the spread of the pathogen was slower in the AtNPR1-transformants compared to the wild type plants. Transcript analysis in the seedling root and hypocotyl showed that the transgenic lines are capable of launching a stronger defense response when infected with Fov11. We further confirmed that AtNPR1 transformants showed greater degree of tolerance to Fov11. However, little or no protection was observed against a related, but more virulent isolate, Fov43, and a highly virulent isolate, CA9.

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Section: Discussionmentioning

confidence: 58%

Section: Introductionmentioning

confidence: 99%

Response of AtNPR1-expressing cotton plants to Fusarium oxysporum f. sp. vasinfectum isolates

Joshi

Kumar

Janga

et al. 2017

Physiol Mol Biol Plants

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“…The potential of over-expression of AtNPR1 from Arabidopsis thaliana or of its orthologues in crop species is a current approach for the development of more resistant cultivars. Over-expression of the AtNPR1 gene in citrus and of the MpNPR1 gene in apple increases resistance to citrus canker (Zhang et al, 2010) and to fire blight (Malnoy et al, 2007), respectively. In some cases negative impacts of the NPR1 expression have been observed in transgenic plants.…”

Section: Exogenous Application Of Natural Compounds Stimulating Plantmentioning

confidence: 99%

The Millardetian Conjunction in the Modern World

Rivière¹,

Ponchet²,

Galiana³

2011

Pesticides in the Modern World - Pesticides Use and Management

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“…nptII− (Norelli et al 1994) contained a nptII-based expression cassette as a selectable marker, an attacin gene driven by the Pin2 promoter, and a uidA intron expression cassette driven by the 35S promoter adjacent to the right border of the T-DNA. pBinMpNPR1 (Malnoy et al 2007) contained an nptII-based expression cassette as a selectable marker and the Malus x domestica Mp-NPR1 gene under the control of the pPin2 promoter. The nptII-based expression cassette of each of these two binary vectors was eliminated using the restriction enzymes, AscI/BsshII and NheI/ClaI, respectively, to produce 1 and 2.…”

Section: Plasmid Constructsmentioning

confidence: 99%

“…In order to evaluate relative differences in cDNAs between transgenic clones, comparative kinetic analysis was conducted by PCR using a procedure described by Malnoy et al (2007). The quantification was done after 15 PCR cycles with −RT controls, in which the RT enzyme was omitted from the RT reaction in order to show that no genomic DNA remained in the samples after DNAse treatment.…”

Section: Semiquantitative Reverse Transcription Pcr Analysismentioning

confidence: 99%

Genetic transformation of apple (Malus x domestica) without use of a selectable marker gene

Malnoy

Boresjza-Wysocka

Norelli

et al. 2010

Tree Genetics & Genomes

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Selectable marker genes are widely used for the efficient transformation of crop plants. In most cases, antibiotic or herbicide resistance marker genes are preferred because they tend to be most efficient. Due mainly to consumer and grower concerns, considerable effort is being put into developing strategies (site-specific recombination, homologous recombination, transposition, and cotransformation) to eliminate the marker gene from the nuclear or chloroplast genome after selection. For the commercialization of genetically transformed plants, use of a completely marker-free technology would be desirable, since there would be no involvement of antibiotic resistance genes or other marker genes with negative connotations for the public. With this goal in mind, a technique for apple transformation was developed without use of any selectable marker. Transformation of the apple genotype "M.26" with the constructs pPin2Att35SGUSintron and pPin2MpNPR1 was achieved. In different experiments, 22.0-25.4% of regenerants showed integration of the gene of interest. Southern analysis in some transformed lines confirmed the integration of one copy of the gene. Some of these transformed lines have been propagated and used to determine the uniformity of transformed tissues in the plantlets. The majority of the lines are uniformly transformed plants, although some lines are chimeric, as also occurs with the conventional transformation procedure using a selectable marker gene. A second genotype of apple, "Galaxy," was also transformed with the same constructs, with a transformation efficiency of 13%.

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Over-Expression of the Apple Gene Mpnpr1 Causes Increased Disease Resistance in Malus X Domestica

Cited by 35 publications

References 0 publications

Response of AtNPR1-expressing cotton plants to Fusarium oxysporum f. sp. vasinfectum isolates

Response of AtNPR1-expressing cotton plants to Fusarium oxysporum f. sp. vasinfectum isolates

The Millardetian Conjunction in the Modern World

Genetic transformation of apple (Malus x domestica) without use of a selectable marker gene

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