Over-expression of stress protein-encoding genes helps Clostridium acetobutylicum to rapidly adapt to butanol stress

Mann, Miriam S.; Dragovic, Zdravko; Schirrmacher, Georg; Lütke‐Eversloh, Tina

doi:10.1007/s10529-012-0951-2

Cited by 61 publications

(35 citation statements)

References 45 publications

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“…The gene Cthe_0550 is annotated as encoding Hsp90 and was up regulated after all stress treatment (Additional file 1: Table S1). Overexpression of htpG was recently shown to enhance butanol tolerance and eventual strain adaptation to butanol in C. acetobutylicum [45] and could provide a route for enhanced solvent resistance in C. thermocellum. …”

Section: Discussionmentioning

confidence: 99%

Clostridium thermocellum transcriptomic profiles after exposure to furfural or heat stress

Wilson

Yang

Rodríguez

et al. 2013

Biotechnol Biofuels

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BackgroundThe thermophilic anaerobe Clostridium thermocellum is a candidate consolidated bioprocessing (CBP) biocatalyst for cellulosic ethanol production. It is capable of both cellulose solubilization and its fermentation to produce lignocellulosic ethanol. Intolerance to stresses routinely encountered during industrial fermentations may hinder the commercial development of this organism. A previous C. thermocellum ethanol stress study showed that the largest transcriptomic response was in genes and proteins related to nitrogen uptake and metabolism.ResultsIn this study, C. thermocellum was grown to mid-exponential phase and treated with furfural or heat to a final concentration of 3 g.L-1 or 68°C respectively to investigate general and specific physiological and regulatory stress responses. Samples were taken at 10, 30, 60 and 120 min post-shock, and from untreated control fermentations, for transcriptomic analyses and fermentation product determinations and compared to a published dataset from an ethanol stress study. Urea uptake genes were induced following furfural stress, but not to the same extent as ethanol stress and transcription from these genes was largely unaffected by heat stress. The largest transcriptomic response to furfural stress was genes for sulfate transporter subunits and enzymes in the sulfate assimilatory pathway, although these genes were also affected late in the heat and ethanol stress responses. Lactate production was higher in furfural treated culture, although the lactate dehydrogenase gene was not differentially expressed under this condition. Other redox related genes such as a copy of the rex gene, a bifunctional acetaldehyde-CoA/alcohol dehydrogenase and adjacent genes did show lower expression after furfural stress compared to the control, heat and ethanol fermentation profiles. Heat stress induced expression from chaperone related genes and overlap was observed with the responses to the other stresses. This study suggests the involvement of C. thermocellum genes with functions in oxidative stress protection, electron transfer, detoxification, sulfur and nitrogen acquisition, and DNA repair mechanisms in its stress responses and the use of different regulatory networks to coordinate and control adaptation.ConclusionsThis study has identified C. thermocellum gene regulatory motifs and aspects of physiology and gene regulation for further study. The nexus between future systems biology studies and recently developed genetic tools for C. thermocellum offers the potential for more rapid strain development and for broader insights into this organism’s physiology and regulation.

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Section: Discussionmentioning

confidence: 99%

Clostridium thermocellum transcriptomic profiles after exposure to furfural or heat stress

Wilson

Yang

Rodríguez

et al. 2013

Biotechnol Biofuels

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“…Organic solvents cause protein damage leading to misfolding. It has been demonstrated that overexpression of heat shock proteins, such as the GroESL system in Clostridium acetobutylicum (a butanol-producing bacteria), results in improved solvent tolerance and solvent production (132). HSP genes can be found in a broad range of antibiotic-resistant plasmids from the Enterobacteriaceae family and in plasmids involved in rhizobia-plant symbiosis.…”

Section: Plasmids Involved In Solvent/aromatic Compound Tolerancementioning

confidence: 99%

Plasmid-Mediated Tolerance Toward Environmental Pollutants

Segura

Molina²,

Ramos

2014

Microbiol Spectr

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The survival capacity of microorganisms in a contaminated environment is limited by the concentration and/or toxicity of the pollutant. Through evolutionary processes, some bacteria have developed or acquired mechanisms to cope with the deleterious effects of toxic compounds, a phenomenon known as tolerance. Common mechanisms of tolerance include the extrusion of contaminants to the outer media and, when concentrations of pollutants are low, the degradation of the toxic compound. For both of these approaches, plasmids that encode genes for the degradation of contaminants such as toluene, naphthalene, phenol, nitrobenzene, and triazine or are involved in tolerance toward organic solvents and heavy metals, play an important role in the evolution and dissemination of these catabolic pathways and efflux pumps. Environmental plasmids are often conjugative and can transfer their genes between different strains; furthermore, many catabolic or efflux pump genes are often associated with transposable elements, making them one of the major players in bacterial evolution. In this review, we will briefly describe catabolic and tolerance plasmids and advances in the knowledge and biotechnological applications of these plasmids.

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“…With the increasing supply of fossil fuel resources and continuous improvements in environmental protection awareness, the production of biofuels from renewable raw materials has become a global priority. Butanol is considered a potential new alternative biofuel and its production through fermentation has received renewed interest in recent years . At present, the genus Clostridium including C. acetobutylicum and C. beijerinckii has been extensively studied for its specific ability to biosynthesize alcohols (butanol and ethanol) and acetone.…”

Section: Introductionmentioning

confidence: 99%

The biological mechanisms of butanol tolerance and the application of solvent‐tolerant bacteria for environmental protection

Gao

Zhang

Guo

et al. 2019

J of Chemical Tech & Biotech

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Toxicity caused by the accumulation of butanol in fermentation media is an important factor limiting the concentration of butanol. There is currently no systematic research in place investigating the butanol tolerance mechanism of bacteria such as Clostridium acetobutylicum, which adapts to butanol stress and regulates its growth and metabolism. Here, research results related to the butanol tolerance of C. acetobutylicum are reviewed to understand the molecular basis of changes in butanol‐tolerant strains. Organic solvent‐tolerant bacteria play an important role in the fields of biofuel production, enzyme preparation and bioremediation. An analysis of limitations of the application of organic solvent‐tolerant bacteria has revealed that future research should focus on combining the microbial tolerance phenotype with specific utilization to achieve an optimal balance between organic solvent tolerance and production. This review serves as a reference for the improvement and engineering of strains that tolerate organic solvents. © 2019 Society of Chemical Industry

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Over-expression of stress protein-encoding genes helps Clostridium acetobutylicum to rapidly adapt to butanol stress

Cited by 61 publications

References 45 publications

Clostridium thermocellum transcriptomic profiles after exposure to furfural or heat stress

Clostridium thermocellum transcriptomic profiles after exposure to furfural or heat stress

Plasmid-Mediated Tolerance Toward Environmental Pollutants

The biological mechanisms of butanol tolerance and the application of solvent‐tolerant bacteria for environmental protection

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