Over-expression of strawberry d-galacturonic acid reductase in potato leads to accumulation of vitamin C with enhanced abiotic stress tolerance

Hemavathi,; Upadhyaya, Chandrama Prakash; Young, Ko Eun; Nookaraju, Akula; Kim, Hyun Soon; Heung, Jeon Jae; Oh, Oh Man; Aswath, C.; Chun, Se Chul; Kim, Doo Hwan; Park, Se Won

doi:10.1016/j.plantsci.2009.08.004

Cited by 120 publications

(62 citation statements)

References 37 publications

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“…Overexpression of two members of the GME gene family resulted in increased accumulation of ascorbate and improved tolerance to abiotic stresses in tomato plants [128]. Overexpression of strawberry D-galacturonic acid reductase which participates in AsA biosynthetic pathway involving D-galacturonic acid as intermediate and reduces D-galacturonic acid to L-galactonic acid, leads to accumulation of AsA and enhanced abiotic stress tolerance in potato plants [129]. Increased AsA content has been shown to confer oxidative stress tolerance in Arabidopsis [130].…”

Section: Nonenzymatic Components Of Antioxidative Defense Systemmentioning

confidence: 99%

Reactive Oxygen Species, Oxidative Damage, and Antioxidative Defense Mechanism in Plants under Stressful Conditions

et al. 2012

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Reactive oxygen species (ROS) are produced as a normal product of plant cellular metabolism. Various environmental stresses lead to excessive production of ROS causing progressive oxidative damage and ultimately cell death. Despite their destructive activity, they are well-described second messengers in a variety of cellular processes, including conferment of tolerance to various environmental stresses. Whether ROS would serve as signaling molecules or could cause oxidative damage to the tissues depends on the delicate equilibrium between ROS production, and their scavenging. Efficient scavenging of ROS produced during various environmental stresses requires the action of several nonenzymatic as well as enzymatic antioxidants present in the tissues. In this paper, we describe the generation, sites of production and role of ROS as messenger molecules as well as inducers of oxidative damage. Further, the antioxidative defense mechanisms operating in the cells for scavenging of ROS overproduced under various stressful conditions of the environment have been discussed in detail.

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Section: Nonenzymatic Components Of Antioxidative Defense Systemmentioning

confidence: 99%

Reactive Oxygen Species, Oxidative Damage, and Antioxidative Defense Mechanism in Plants under Stressful Conditions

et al. 2012

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“…The T-DNA region of the plasmid vector contains GLOase and nptII genes driven under the control of CaMV35S promoter. Transgenic plants were generated via Agrobacterium tumefaciens mediated transformation, as described (Hemavathi et al 2009). The regenerated shoots were transferred to MS basal medium supplemented with kanamycin (50 mg l -1 ) and carbenicillin (300 mg l -1 ) for rooting.…”

Section: Plant Materialsmentioning

confidence: 99%

“…These include L-gulono-c-lactone oxidase (GLOase) cDNA isolated from mouse in tobacco and lettuce (Jain and Nessler 2000), a human dehydroascorbate (DHAR) gene in tobacco (Kwon et al 2003), Dgalacturonic acid reductase (GalUR) cDNA of strawberry in Arabidopsis and potato (Agius et al 2003;Hemavathi et al 2009), and wheat DHAR cDNA in tobacco and maize (Chen et al 2003).…”

Section: Introductionmentioning

confidence: 99%

Enhanced ascorbic acid accumulation in transgenic potato confers tolerance to various abiotic stresses

et al. 2009

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L-ascorbic acid (Vitamin C, AsA) is an important component of human nutrition. Plants and several animals can synthesize their own ascorbic acid, whereas humans lack the gene essential for ascorbic acid biosynthesis and must acquire from their diet. In the present study, we developed transgenic potato (Solanum tuberosum L. cv. Taedong Valley) over-expressing L-gulono-gamma-lactone oxidase (GLOase gene; NCBI Acc. No. NM022220), isolated from rat cells driven by CaMV35S constitutive promoter that showed enhanced AsA accumulation. Molecular analyses of four independent transgenic lines performed by PCR, Southern and RT-PCR revealed the stable integration of the transgene in the progeny. The transformation frequency was ca. 7.5% and the time required for the generation of transgenic plants was 6-7 weeks. Transgenic tubers showed significantly enhanced AsA content (141%) and GLOase activity as compared to untransformed tubers. These transgenics were also found to withstand various abiotic stresses caused by Methyl Viologen (MV), NaCl or mannitol, respectively. The T(1) transgenic plants exposed to salt stress (100 mM NaCl) survived better with increased shoot and root length when compared to untransformed plants. The elevated level of AsA accumulation in transgenics was directly correlated with their ability to withstand abiotic stresses. These results further demonstrated that the overexpression of GLOase gene enhanced basal levels of AsA in potato tubers and also the transgenics showed better survival under various abiotic stresses.

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“…Humans are incapable of synthesizing AsA and must acquire it from their diet, typically from fresh fruits and vegetables. As a result, there is considerable interest in enhancing AsA content in a wide variety of food crops, and transgenic corn, potato, lettuce, and tomato with elevated AsA have been developed [1][2][3][4]. However, relatively little consideration has been given to how biotic stresses such as insect damage influence AsA content in plants, or, conversely, how modification of AsA content could influence plant susceptibility to these stresses.…”

Section: Introductionmentioning

confidence: 99%

Vitamin C content in plants is modified by insects and influences susceptibility to herbivory

2010

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Analysis of a diverse cross-sample of plant-insect interactions suggests that the abundance of vitamin C (L-ascorbic acid, ascorbate or AsA) in plants influences their susceptibility to insect feeding. These effects may be mediated by AsAs roles as an essential dietary nutrient, as an antioxidant in the insect midgut, or as a substrate for plant-derived ascorbate oxidase, which can lead to generation of toxic reactive oxygen species. Ascorbate can also influence the efficacy of plant defenses such as myrosinases and tannins, and alter insects' susceptibility to natural enemies. Conversely, herbivores appear to influence both de novo synthesis and redox cycling of AsA in their host plants, thereby potentially altering the nutritional value of crops and their susceptibility to pests. The recent development of genetically modified crops with enhanced AsA content provides both an impetus and a tool set for further studies on the role of AsA in plant-insect interactions.

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Over-expression of strawberry d-galacturonic acid reductase in potato leads to accumulation of vitamin C with enhanced abiotic stress tolerance

Cited by 120 publications

References 37 publications

Reactive Oxygen Species, Oxidative Damage, and Antioxidative Defense Mechanism in Plants under Stressful Conditions

Reactive Oxygen Species, Oxidative Damage, and Antioxidative Defense Mechanism in Plants under Stressful Conditions

Enhanced ascorbic acid accumulation in transgenic potato confers tolerance to various abiotic stresses

Vitamin C content in plants is modified by insects and influences susceptibility to herbivory

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