Over-expression of rice leucine-rich repeat protein results in activation of defense response, thereby enhancing resistance to bacterial soft rot in Chinese cabbage

Abstract

Pectobacterium carotovorum subsp. carotovorum causes soft rot disease in various plants, including Chinese cabbage. The simple extracellular leucine-rich repeat (eLRR) domain proteins have been implicated in disease resistance. Rice leucine-rich repeat protein (OsLRP), a rice simple eLRR domain protein, is induced by pathogens, phytohormones, and salt. To see whether OsLRP enhances disease resistance to bacterial soft rot, OsLRP was introduced into Chinese cabbage by Agrobacterium-mediated transformation. Two independent transgenic lines over-expressing OsLRP were generated and further analyzed. Transgenic lines over-expressing OsLRP showed enhanced disease resistance to bacterial soft rot compared to non-transgenic control. Bacterial growth was retarded in transgenic lines over-expressing OsLRP compared to non-transgenic controls. We propose that OsLRP confers enhanced resistance to bacterial soft rot. Monitoring expression of defense-associated genes in transgenic lines over-expressing OsLRP, two different glucanases and Brassica rapa polygalacturonase inhibiting protein 2, PDF1 were constitutively activated in transgenic lines compared to non-transgenic control. Taken together, heterologous expression of OsLRP results in the activation of defense response and enhanced resistance to bacterial soft rot.

Simultaneous Transcriptome Analysis of Sorghum and Bipolaris sorghicola by Using RNA-seq in Combination with De Novo Transcriptome Assembly

“…We found the same characteristics in SLRR2 (Figure 2B and C). Two groups have reported that OsLRR1–a member of the family in rice–enhances disease resistance when ectopically expressed in Arabidopsis and Chinese cabbage [40], [43]. We therefore conclude that SLRR2 is a new member of the simple eLRR domain protein family and that SLRR and SLRR2 are involved in the defense response.…”

“…Recently, some groups have proposed that SLRR belongs to an LRR subfamily called the simple eLRR domain protein family [40]–[43]. Although members of the family are structurally similar to the LRR-only receptor PGIP, they show greater sequence identity to the LRR domains of receptor-like kinases such as SERK1, SERK2, and BAK1.…”

Identification of boron-deficiency-responsive microRNAs in Citrus sinensis roots by Illumina sequencing

“…qRT-PCR analysis showed that B-deficient roots had enhanced expression of LRP , but decreased expression of sulfate transporter (Table 3). Park et al [83] observed that heterologous expression of rice LRP ( OsLRP ) resulted in the activation of defense response and enhanced resistance to bacterial soft rot in Chinese cabbage. Thus, the higher expression of LRP might partially compensate for the decreased disease resistance in B-deficient plants [66].…”

Silencing of the Rice Gene LRR1 Compromises Rice Xa21 Transcript Accumulation and XA21-Mediated Immunity

“…tomato DC3000 ( Pst DC3000) (Zhou et al 2009; Zhou et al 2010) and Pectobacterium carotovorum subsp. carotovorum (Park et al 2012) respectively. In contrast to these results, we found overexpression of LRR1 in the Kitaake rice variety did not enhance resistance to Xoo (Fig.…”