Over-expression of poplar transcription factor ERF76 gene confers salt tolerance in transgenic tobacco

Yao, Wenjing; Wang, Lei; Zhou, Boru; Wang, Shengji; Li, Renhua; Jiang, Tongmin

doi:10.1016/j.jplph.2016.03.015

Cited by 43 publications

(27 citation statements)

References 55 publications

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“…Over-expression of the poplar transcription factor ERF76 gene in tobacco enhanced its salt tolerance. Compared to the control, the transgenic line showed a significant increase in seed germination rate, plant height, root length, and fresh weight, as well as in relative water content, activity of antioxidant enzymes, and proline content after salt stress [65]. The role of ERFs in the response of C. inerme roots to salt stress needs additional verification.…”

Section: Discussionmentioning

confidence: 95%

RNA-Seq analysis of Clerodendrum inerme (L.) roots in response to salt stress

et al. 2019

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Background Clerodendrum inerme (L.) Gaertn, a halophyte, usually grows on coastal beaches as an important mangrove plant. The salt-tolerant mechanisms and related genes of this species that respond to short-term salinity stress are unknown for us. The de novo transcriptome of C. inerme roots was analyzed using next-generation sequencing technology to identify genes involved in salt tolerance and to better understand the response mechanisms of C. inerme to salt stress. Results Illumina RNA-sequencing was performed on root samples treated with 400 mM NaCl for 0 h, 6 h, 24 h, and 72 h to investigate changes in C. inerme in response to salt stress. The de novo assembly identified 98,968 unigenes. Among these unigenes, 46,085 unigenes were annotated in the NCBI non-redundant protein sequences (NR) database, 34,756 sequences in the Swiss-Prot database and 43,113 unigenes in the evolutionary genealogy of genes: Non-supervised Orthologous Groups (eggNOG) database. 52 Gene Ontology (GO) terms and 31 Kyoto Encyclopedia of Genes and Genomes (KEGG) pathways were matched to those unigenes. Most differentially expressed genes (DEGs) related to the GO terms “single-organism process”, “membrane” and “catalytic activity” were significantly enriched while numerous DEGs related to the plant hormone signal transduction pathway were also significantly enriched. The detection of relative expression levels of 9 candidate DEGs by qRT-PCR were basically consistent with fold changes in RNA sequencing analysis, demonstrating that transcriptome data can accurately reflect the response of C. inerme roots to salt stress. Conclusions This work revealed that the response of C. inerme roots to saline condition included significant alteration in response of the genes related to plant hormone signaling. Besides, our findings provide numerous salt-tolerant genes for further research to improve the salt tolerance of functional plants and will enhance research on salt-tolerant mechanisms of halophytes.

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Section: Discussionmentioning

confidence: 95%

RNA-Seq analysis of Clerodendrum inerme (L.) roots in response to salt stress

et al. 2019

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“…A number of studies reveal that the ERF subfamily members in various plant species, acting as either transcriptional activators or repressors, play a pivotal role in regulation of a range of physiological and biological processes, including internode elongation , root growth (Jung et al, 2017), trichome formation (Sun et al, 2017), fruit ripening (Yin et al, 2016), hormone signal transduction (Rashotte et al, 2006), secondary metabolism and biotic stress response (Zeng et al, 2015;Zhu et al, 2014). Accumulating evidence demonstrate that ERFs are also implicated in response to abiotic stresses, including submergence or hypoxia (Xu et al, 2006), heavy metal (Lin et al, 2017), drought (Jung et al, 2017) and high salinity (Yao et al, 2016). ERFs have been also demonstrated to be responsive to cold stress.…”

Section: Introductionmentioning

confidence: 99%

ERF109 of trifoliate orange (Poncirus trifoliata (L.) Raf.) contributes to cold tolerance by directly regulating expression of Prx1 involved in antioxidative process

Wang

Dai

et al. 2019

Plant Biotechnology Journal

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Summary Ethylene‐responsive factors ( ERF s) have been revealed to play essential roles in a variety of physiological and biological processes in higher plants. However, functions and regulatory pathways of most ERF s in cold stress remain largely unclear. Here, we identified Ptr ERF 109 of trifoliate orange ( Poncirus trifoliata (L.) Raf.) and deciphered its role in cold tolerance. Ptr ERF 109 was drastically up‐regulated by cold, ethylene and dehydration, but repressed by salt. Ptr ERF 109 was localized in the nucleus and displayed transcriptional activity, and the C terminus is required for the activation. Overexpression of Ptr ERF 109 conferred enhanced cold tolerance in transgenic tobacco and lemon plants, whereas VIGS (virus‐induced gene silencing)‐mediated suppression of Ptr ERF 109 in trifoliate orange led to increased cold susceptibility. Ptr ERF 109 overexpression caused extensive transcriptional reprogramming of several suites of stress‐responsive genes. Prx1 encoding class III peroxidase ( POD ) was one of the antioxidant genes exhibiting the greatest induction. Ptr ERF 109 was shown to directly bind to the promoter of PtrPrx1 (trifoliate orange Prx1 homologue) and positively activated its expression. In addition, the Ptr ERF 109 ‐overexpressing plants exhibited significantly higher POD activity and accumulated dramatically less H 2 O 2 and were more tolerant to oxidative stress, whereas the VIGS plants exhibited opposite trends, in comparison with wild type. Taken together, these results indicate that Ptr ERF 109 as a positive regulator contributes to imparting cold tolerance by, at least partly, directly regulating the POD ‐encoding gene to maintain a robust antioxidant capacity for effectively scavenging the reactive oxygen species. Our findings gain insight into better understanding of transcriptional regulation of antioxidant genes in response to cold stress.

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“…And germinated seeds were transferred into tissue culture bottles containing 1/2 MS solid medium. The aseptic seedlings at one month old were used for gene transformation [32].…”

Section: Discussionmentioning

confidence: 99%

Over-expression of poplar NAC15 gene enhances wood formation in transgenic tobacco

Yao

Zhang

Zhou

et al. 2019

Preprint

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BackgroundNAC (NAM/ATAF/CUC) family is one of the largest plant-specific TF families known to play significant roles in wood formation. Acting as master gene regulators, a few NAC genes can activate secondary wall biosynthesis during wood formation in woody plants.ResultsIn the present study, firstly, we screened 110 differentially expressed NAC genes in the leaves, stems, and roots of di-haploid Populus simonii×P. nigra by RNA-Seq. Then we identified a nucleus-targeted gene, NAC15 gene, which was one of the most highly expressed genes in the stem among NAC family members. Thirdly, we conducted expression pattern analysis of NAC15 gene, and proved NAC15 gene was most highly expressed in the xylem. Moreover, we transferred NAC15 gene into tobacco and obtained 12 transgenic tobacco lines overexpressing the gene. And the relative content of hemicellulose, cellulose and lignin was determined to be higher in the transgenic plants than that in the control plants. It also showed darker staining in the culms of the transgenic plants with phloroglucinol staining, compared to the control plants. Furthermore, the relative expression level of a few lignin- and cellulose-related genes was significantly higher in the TLs than that in the CLs.ConclusionsThe overall results indicated that NAC15 gene is highly expressed in the xylem of poplar and plays an important role in wood formation in transgenic tobacco, which provides a foundation for the identification of wood-associated downstream genes and has significant implication in tree biology and biotechnology.

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Over-expression of poplar transcription factor ERF76 gene confers salt tolerance in transgenic tobacco

Cited by 43 publications

References 55 publications

RNA-Seq analysis of Clerodendrum inerme (L.) roots in response to salt stress

RNA-Seq analysis of Clerodendrum inerme (L.) roots in response to salt stress

ERF109 of trifoliate orange (Poncirus trifoliata (L.) Raf.) contributes to cold tolerance by directly regulating expression of Prx1 involved in antioxidative process

Over-expression of poplar NAC15 gene enhances wood formation in transgenic tobacco

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