Over Expression of p5cs Gene to Increase Cold Stress Tolerance in Iranian Species of Petunia

Abstract

In order to examine the relationship between proline and cold stress in Iranian (local) genotype of petunia, p5cs gene was transferred to petunia through Agrobacterium-mediated transformation. The leaf discs from four weeks and shoot apices from 7-day-old petunia in vitro plants were co-cultivated with Agrobacterium strain LBA4404 harboring a plasmid pBI121 as the vector system for transformation of petunia. pBI121 plasmid containing β-glucuronidase (gus) gene as a reporter gene and Δ1-pyrroline-5-carboxylate synthetase (p5cs) gene and neomycin phosphotransferase (nptII) gene were used as a selectable marker. The co-cultivated leaf discs and shoot apices were transferred to the selective medium and thereafter to proliferation medium respectively. To confirm transformation, regenerated plants were subjected to the polymerase chain reaction (PCR), GUS histochemical and proline assays. The results confirmed the presence of the gus and p5cs gene in the genome of all transformants and the transformed plants were more tolerant (674.87 μg/g fresh leaves) than the wild types under stress conditions.

Overexpression of sugarcane SoTUA gene enhances cold tolerance in transgenic sugarcane

“…It has been previously proven that P5CS was a gene encoding the key enzyme involved in proline (Rai & Penna, 2013;Signorelli & Monza, 2017); however, the proline accumulation takes part in response to many abiotic stresses, thus the P5CS gene plays a key role in stress tolerance for growth and development of plants. It has been reported that overexpression of P5CS enhances the abiotic stress resistance of plant, such as LT (Jamshidnia et al, 2018), salt (Guan et al, 2018), drought stress (Wei at al. et al, 2016), and so on.…”