Over-expression of Mn-superoxide dismutase as a marker of oxidative stress in lesional skin of chronic idiopathic urticaria

Abstract

We studied the involvement of oxidative stress in chronic idiopathic urticaria (CIU), assessing the activities of superoxide dismutase (SOD) and glutathione and the levels of malondialdeyde (MDA), a marker of lipid peroxidation, in samples taken from lesional skin (n = 16) and nonlesional skin (n = 11) of CIU patients. The activity of SOD and glutathione and the levels of MDA were markedly increased in lesional skin as compared with skin of healthy subjects, whereas no differences were detected between nonlesional skin of CIU patients and control samples. Immuno-dot blot assay revealed an up-regulation of Mn-SOD expression in lesional skin. These findings show that oxidative stress is crucially involved in CIU. The evidence of lipid peroxidation and compensatory increase of Mn-SOD and glutathione activities in lesional skin, in the absence of any alteration in uninvolved skin, suggests that oxidative stress is secondary to the development of inflammation.

Oxidant Stress and Antioxidant Status Among Patients With Nonalcoholic Fatty Liver Disease (NAFLD)

“…37 Nonenzymatic glycation of EC-SOD reduces its endothelial binding resulting in an increase of free EC-SOD levels and this may be a major cause of this phenomenon. It has been shown that in diseases, whose pathogenesis involves chronic oxidative stress, SOD levels are increased, as [38][39][40][41][42] Also, it is known that at the time of cell injury a large amount of SOD is released, 43 which may explain the lack of difference of SOD levels between cases and diseased controls. Previous studies have demonstrated positive correlation of lipid peroxidation products and negative correlation of antioxidant levels with histological severity of NAFLD, although in some studies this association was very weak 27,29 or absent.…”

Oxidative Stress in Allergic and Inflammatory Skin Diseases

“…However, increased SOD activity has also been observed [8]. The activity of SOD, glutathione, and the levels of malondialdehyde (a marker of lipid peroxidation) were recently reported to be increased markedly in samples taken from the skin lesions of patients with chronic idiopathic urticaria, compared with the values obtained in skin from healthy subjects [9]. However, no differences were detected between non-lesional skin samples from patients with chronic idiopathic urticaria and control samples.…”

“…Furthermore, treatment response was associated with significant reductions in the concentrations of 8-OHdG and acrolein-lysine adducts [5]. Antioxidants 9,11] low-molecular-weight antioxidants bilirubin oxidative metabolites (u)…”

The Role of Platelets in Chronic Urticaria

“…Platelets release ROS upon activation and these ROS might influence the activation and degranulation of basophils and mast cells [82,83]. One study has suggested the existence of significant systemic inflammation and platelet oxidative stress in patients with CU [74].…”