Over-expression of long noncoding RNA BANCR inhibits malignant phenotypes of human bladder cancer

Abstract

BackgroundAccumulating evidences indicated that lncRNAs play crucial regulatory roles in oncogenesis and progression of cancers. BRAF activated non-coding RNA (BANCR) has been identified to contribute to the progression of some human cancers. However, the relationship between BANCR and bladder cancer (BC) is largely unclear.MethodsBANCR expression levels in BC, paired non-cancer tissues and BC cell lines were detected by real-time quantitative RT-PCR (qRT-PCR). The relationships between BANCR expression levels and the clinical characteristics were evaluated. BANCR expression was enhanced by transfecting a pcDNA-BANCR vector. We used both CCK-8 assay and Edu assay to detect cell proliferation. We also detect cell apoptosis and migration by using ELISA assay, Flow cytometry and transwell assay, respectively. All statistical analyses were executed by using the SPSS 20.0 software.ResultsBANCR expression levels were remarkably decreased in BC tissues compared with adjacent noncancerous tissues. BANCR expression levels in two BC cell lines were also significantly down-regulated. Clinicopathologic analysis revealed that low BANCR expression was positively correlated with TNM stage, but not associated with other clinicopathological characteristics. BANCR has been successfully overexpressed in BC cell lines (T24 and SW780) by transfecting a pcDNA-BANCR vector. Cell proliferation inhibition, apoptosis induction and migration suppression were also observed in pCDNA-BANCR-transfected T24 and SW780 cells.ConclusionsThese data suggested that BANCR represents a tumor suppressor player in bladder cancer, contributes to tumor proliferation, apoptosis and migration, and may serve as a new candidate biomarker and a potential therapeutic target for patients with BC.

Long noncoding RNA XIST participates in bladder cancer by downregulating p53 via binding to TET1

“…He 26 found that Overexpression of long noncoding RNA BANCR inhibits malignant phenotypes of human bladder cancer. As Li 25 pointed out, increased expression of lncRNA H19 can significantly promote cell proliferation and cell migration in bladder cancer.…”

Long non‐coding RNAs in melanoma

“…BRAF‐activated non‐coding RNA (BANCR) is a 4‐exon transcript of 693 bp, whose encoding gene is located on chromosome 9 . BANCR is involved in a variety of human malignancies, including lung carcinoma, colorectal cancer, melanoma, gastric cancer and bladder cancer . Nevertheless, the direction of deregulation of BANCR was tissue‐specific in which this lncRNA could act as an oncogene or tumour‐suppressor gene .…”

The BRAF activated non‐coding RNA: A pivotal long non‐coding RNA in human malignancies

“…The expression level of BANCR RNA was also downregulated in BC cell lines (SW780 and T24). Once BANCR was overexpressed with pcDNA‐BANCR vector in BC cells, cell proliferation was inhibited, apoptosis was induced and migration was suppressed significantly . These results indicate that BANCR participates in BC as a tumour suppressor, and it may serve as a potential biomarker and candidate therapeutic target for BC.…”

“…Lack of thorough understanding of underlying molecular mechanism partially accounts for the high fatality rate of BC. The expression level of BANCR RNA was found to be dramatically downregulated in human BC tissues, and decreased BANCR was revealed to be positively correlated with the TNM stage by clinicopathologic analysis . The expression level of BANCR RNA was also downregulated in BC cell lines (SW780 and T24).…”