Over-Expression of EphA2 and EphrinA-1 in Human Gastric Adenocarcinoma and Its Prognostic Value for Postoperative Patients

Abstract

This study aims to investigate the expression and significance of EphA2 and EphrinA-1 in human gastric adenocarcinoma progression and prognosis. The expression of EphA2 and EphrinA-1 was detected in the cell lines and tissues of gastric adenocarcinoma. Different expression levels of EphA2 and EphrinA-1 were found in two cell lines. The expression of EphA2 and EphrinA-1 was significantly higher in gastric adenocarcinoma tissues than in normal tissues. Statistical analysis showed a significant correlation of EphA2 expression with the depth of tumor invasion, tumor-node-metastasis (TNM) stages, and lymph node metastasis. EphrinA-1 over-expression was significantly correlated with TNM stages and lymph node metastasis, while EphA2 expression was found to be an independent prognostic factor of postoperative gastric adenocarcinoma. In conclusion, the increased expression of EphA2 and EphrinA-1 plays an important role in the progression of human gastric adenocarcinoma, in which elevated EphA2 expression is an independent factor that indicates poor prognosis in postoperative gastric adenocarcinoma.

Clinical significance of EphA2 expression in squamous-cell carcinoma of the head and neck

“…However, the underlying mechanisms for the high expression of EphA2 protein in tumor remain incompletely understood but likely involve both transcriptional and posttranscriptional mechanisms. Our data that both mRNA and protein expression of EphA2 were elevated in SCCHN tissue samples and similar Wndings in gastric carcinoma (Yuan et al 2009) indicated the pre-transcriptional mechanism in SCCHN. However, other studies hypothesized that the unstable cell to cell contacts in malignant cells could functionally decrease EphA2 binding possibility with its membrane-anchored ligands, further preventing ligand-mediated degradation, which contributes to the high expression of EphA2 in tumor cells (WalkerDaniels et al 2002;Kikawa et al 2002).…”

Irradiation affects cellular properties and Eph receptor expression in human melanoma cells

“…26,27 High levels of EphA2 were also found in breast cancer, 28,29 prostate cancer, 30 lung cancer, 31 malignant glioma 32 and gastric cancer. 33 Although the EphA2/ephrinA1 pathway can be linked to tumor neovascularization 34 and the formation of tubular structures in metastatic melanoma, 35 most data reveal its role in cell proliferation and modulation of the cytoskeleton. Activation of EphA2 receptor negatively regulates the growth and survival of different tumor cell models.…”

The Value of HER2 neu and EphA2 expressions in Gastric Adenocarcinoma Prognosis

“…We used score 3 as a cut off value above which was considered as over expression and below which was considered as low expression [9].…”

“…Studies addressing the association between HER2-neu expression and GAC patient's prognosis were conflicting as some studies demonstrated positive correlation between HER2-neu expression and GAC patient's prognosis while other studies failed to demonstrate similar results [6][7][8]. Erythropoietin-producing hepatocellular (Eph) molecules are major detected RTKs members that is detected at lower levels in non-neoplastic-epithelial cells [9], and it has many different functions in cancers as tumor initiation, progression, angiogenesis and spread [10,11]. EphA2 receptor is a RTK-Ras signaling component [12].…”