Over-expression of Dof-type transcription factor increases lipid production in Chlamydomonas reinhardtii

Ibáñez-Salazar, Alejandro; Rosales‐Mendoza, Sergio; Rocha‐Uribe, Alejandro; Ramírez-Alonso, Jocelin Itzel; Lara-Hernández, I.; Hernández-Torres, Araceli; Paz-Maldonado, Luz María Teresita; Silva‐Ramírez, Ana Sonia; Bañuelos-Hernández, Bernardo; Martínez-Salgado, José Luis; Soria-Guerra, Ruth Elena

doi:10.1016/j.jbiotec.2014.05.003

Cited by 77 publications

(40 citation statements)

References 45 publications

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“…In vascular cells, ppDof5 activates the promoter of GS1b, whose product is involved in generating glutamine for nitrogen transport and recycling the ammonium released in lignin biosynthesis, whereas, in the chlorophyllous parenchyma of photosynthetic tissues, ppDof5 acts as a transcriptional repressor of GS1a, which is expressed almost exclusively in this cell type and whose product is probably associated with ammonium assimilation in photosynthetic cells (rueda-lópez et al, 2008). similarly, overexpression of CrDof increased the amount of lipids in C. reinhardtii by around twofold (Ibáñez-salazara et al, 2014). similarly, overexpression of CrDof increased the amount of lipids in C. reinhardtii by around twofold (Ibáñez-salazara et al, 2014).…”

Section: Regulation Of Metabolismmentioning

confidence: 81%

Structure, Function, and Evolution of the Dof Transcription Factor Family

Yanagisawa

2016

Plant Transcription Factors

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Section: Regulation Of Metabolismmentioning

confidence: 81%

Structure, Function, and Evolution of the Dof Transcription Factor Family

Yanagisawa

2016

Plant Transcription Factors

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“…[64] Synthetic soybean Dof-like TFs elevated the expression of genes involved in FA, phospholipid and glycolipid biosynthesis, such as enoyl-ACP-reductase, KASII, sulfolipid synthase, and MGD1 in transgenic C. reinhardtii. [84] …”

Section: Wrinkledmentioning

confidence: 99%

Transport and transcriptional regulation of oil production in plants

Manan

Chen

She

et al. 2016

Critical Reviews in Biotechnology

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Triacylglycerol (TAG) serves as an energy reservoir and phospholipids as build blocks of biomembrane to support plant life. They also provide human with foods and nutrients. Multi-compartmentalized biosynthesis, trafficking or cross-membrane transport of lipid intermediates or precursors and their regulatory mechanisms are not fully understood. Recent progress has aided our understanding of how fatty acids (FAs) and phospholipids are transported between the chloroplast, the cytoplasm, and the endoplasmic reticulum (ER), and how the ins and outs of lipids take place in the peroxisome and other organelles for lipid metabolism and function. In addition, information regarding the transcriptional regulation network associated with FA and TAG biosynthesis has been further enriched. Recent breakthroughs made in lipid transport and transcriptional regulation has provided significant insights into our comprehensive understanding of plant lipid biology. This review attempts to highlight the recent progress made on lipid synthesis, transport, degradation, and their regulatory mechanisms. Metabolic engineering, based on these knowledge-powered technologies for production of edible oils or biofuels, is reviewed. The biotechnological application of metabolic enzymes, transcription factors and transporters, for oil production and composition improvement, are discussed in a broad context in order to provide a fresh scenario for researchers and to guide future research and applications.ARTICLE HISTORY

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“…FAMEs (Fatty acids‐methyl esters) were quantified and identified from the lipids previously extracted using a gas chromatographer with a flame ionization detector (FID) (Varian Inc., Walnut Creek, CA) and a Stabilwax capillary column (60 m × 0.25 mm internal diameter, Restek Corp.) as previously described . Quantification of fatty acids as percentage of total dry weight cell was performed using heptadecanoic acid as internal standard (Sigma‐Aldrich Inc., St. Louis, MO).…”

Section: Methodsmentioning

confidence: 99%

pH effects on the lipid and fatty acids accumulation in Chlamydomonas reinhardtii

Ochoa-Alfaro

Gaytán-Luna

González‐Ortega

et al. 2019

Biotechnology Progress

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pH variations influence the delivery of essential nutrients and CO2 solubility, which impact algae metabolism. In this study the microalgal growth and chlorophyll, lipid, and fatty acids content; along with the expression of some genes implicated in the biosynthesis of lipids were examined in Chlamydomonas reinhardtii subjected to pH values of 7.0, 7.8, and 8.5. At pH 7.8 an increase in cell growth was observed with a significant accumulation of chlorophyll (1.75‐fold) when compared with growth at pH 7, while at pH 8.5 a sharp decrease in both parameters was observed when compared with the other pH values tested. Lipid content increased 3.0 (14.81% of dry cell weight, dcw) and 2.3 times (11.43% dcw) at pH 7.8 and 8.5, respectively, when compared with the experiment at pH 7 (4.97% dcw). The compositions of major fatty acids in the strains growing at pH 7.0, 7.8, or 8.5 were 25.7, 28.0, and 32.1% for palmitic acid; 17.3, 14.7, and 25.7% for oleic acid; and 9.8, 12.1, and 4.6% for linoleic acid; respectively. qRT‐PCR analysis showed that the transcripts of ß‐carboxyltransferase, Acyl carrier protein 1, acyl‐ACP thiolase 1, acyl‐sn‐glycerol‐3‐phosphate acyltransferase, and diacylglycerol acyl transferase isoform 3 were significantly induced at pH 7.8 when compared with the other two pH conditions. These results indicate that the induction of genes implicated in the early and final steps of lipid biosynthesis contributes to their accumulation in the stationary phase. Our research suggests that a pH of 7.8 might be ideal to maximize growth and lipid accumulation.

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Over-expression of Dof-type transcription factor increases lipid production in Chlamydomonas reinhardtii

Cited by 77 publications

References 45 publications

Structure, Function, and Evolution of the Dof Transcription Factor Family

Structure, Function, and Evolution of the Dof Transcription Factor Family

Transport and transcriptional regulation of oil production in plants

pH effects on the lipid and fatty acids accumulation in Chlamydomonas reinhardtii

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