Over-Expression of BnMAPK1 in Brassica napus Enhances Tolerance to Drought Stress

Weng, Chang-Mei; Lu, Junxing; Wan, Heng; Wang, Shuwen; Zhen, Wang; Lü, Kun; Liang, Ying

doi:10.1016/s2095-3119(13)60696-6

Cited by 19 publications

(7 citation statements)

References 39 publications

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“…Another example is the abscisic acid (ABA)-activated MAPKKK17/18-MKK3-MAPK1/2/7/14 pathway, which transduces the ABA signal to regulate the expression of a number of ABA-dependent genes [ 35 ]. In previous work, we reported that overexpression of the rapeseed ( Brassica napus ) BnaMAPK1 gene increased plant resistance to drought stress and to the pathogenic fungus Sclerotinia sclerotiorum [ 36 , 37 ]. In contrast to A and B group MAPKs, reports on C groups are scarce.…”

Section: Introductionmentioning

confidence: 99%

Genome-Wide Identification and Analysis of MKK and MAPK Gene Families in Brassica Species and Response to Stress in Brassica napus

Wang

Wan

Meng

et al. 2021

IJMS

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Mitogen-activated protein kinase (MAPK) cascades are common and conserved signal transduction pathways and play important roles in various biotic and abiotic stress responses and growth and developmental processes in plants. With the advancement of sequencing technology, more systematic genetic information is being explored. The work presented here focuses on two protein families in Brassica species: MAPK kinases (MKKs) and their phosphorylation substrates MAPKs. Forty-seven MKKs and ninety-two MAPKs were identified and extensively analyzed from two tetraploid (B. juncea and B. napus) and three diploid (B. nigra, B. oleracea, and B. rapa) Brassica species. Phylogenetic relationships clearly distinguished both MKK and MAPK families into four groups, labeled A–D, which were also supported by gene structure and conserved protein motif analysis. Furthermore, their spatial and temporal expression patterns and response to stresses (cold, drought, heat, and shading) were analyzed, indicating that BnaMKK and BnaMAPK transcript levels were generally modulated by growth, development, and stress signals. In addition, several protein interaction pairs between BnaMKKs and C group BnaMAPKs were detected by yeast two-hybrid assays, in which BnaMKK3 and BnaMKK9 showed strong interactions with BnaMAPK1/2/7, suggesting that interaction between BnaMKKs and C group BnaMAPKs play key roles in the crosstalk between growth and development processes and abiotic stresses. Taken together, our data provide a deeper foundation for the evolutionary and functional characterization of MKK and MAPK gene families in Brassica species, paving the way for unraveling the biological roles of these important signaling molecules in plants.

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Section: Introductionmentioning

confidence: 99%

Genome-Wide Identification and Analysis of MKK and MAPK Gene Families in Brassica Species and Response to Stress in Brassica napus

Wang

Wan

Meng

et al. 2021

IJMS

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“…It was found that overexpression of the maizeZmMAPK1 gene could enhance the drought resistance of transgenicArabidopsis plants (Wu et al, 2015). Weng et al (2014) also found that overexpression of BnMAPK1 in rape could enhance its drought resistance by increasing the water retention of cells and the activity of roots. In this study, the transcriptional levels of DEG encoding MAPK (12 and 9 genes), MAPKK (4 and 4 genes), and MAPKKK (2 and 6 genes) were up-regulated in H. ammodendron and H. persicum .…”

Section: Discussionmentioning

confidence: 98%

Characterization of the gene expression profile response to drought stress in Haloxylon Bunge using PacBio SMRT and Illumina sequencing

Yang

2022

Preprint

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Haloxylon ammodendron and Haloxylon persicum are important drought-tolerant plants in northwest China. The whole-genome sequencing of H. ammodendron and H. persicum grown in their natural environment is incomplete, and their transcriptional regulatory network in response to drought environment remains unclear. To reveal the transcriptional responses of H. ammodendron and H. persicum to an arid environment, we performed single-molecule real-time (SMRT) and Illumina RNA sequencing. In total, 20,246,576 and 908,053 subreads and 435,938 and 210,334 circular consensus sequencing reads were identified by SMRT sequencing of H. ammodendron and H. persicum, and 15238 and 10135 unigenes, respectively, were successfully collected. In addition, 9794 and 7330 simple sequence repeats (SSRs) and 838 and 71 long noncoding RNAs were identified. In an arid environment, the growth of H. ammodendron was restricted; plant height decreased significantly; and the base and branch diameters became thinner. Hydrogen peroxide (H O ) content and peroxidase activity were increased. Under dry and wet conditions, 11,803 and 15,217 differentially expressed genes (DEGs) were identified in H. ammodendron and H. persicum, respectively. There were 319 and 415 DEGs in the signal transduction pathways related to drought stress signal perception and transmission, including the Ca signal pathway, the ABA signal pathway, and the MAPK signal cascade. In addition, 217 transcription factors (TFs) and 398 TFs of H. ammodendron and H. persicum were differentially expressed, including FAR1, MYB, and AP2/ERF. Bioinformatic analysis showed that under drought stress, the expression patterns of genes related to active oxygen (reactive oxygen species) scavenging, functional proteins, lignin biosynthesis, and glucose metabolism pathways were altered. This is the first full-length transcriptome report concerning the responses of H. ammodendron and H. persicum to drought stress. The results provide a foundation for further study of the adaptation to drought stress. The full-length transcriptome can be used in genetic engineering research.

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“…The black-seed doubled haploid inbred B. napus cultivar Zhongyou821 (WT group), three BnaMAPK1 -OE T 3 lines (OE group), and tobacco ( Nicotiana benthamiana ) used in this study were obtained from Southwest University in China. The complete coding sequence of BnaMAPK1 ( BnaA06g06010D ) under the control the CaMV 35S promoter was transformed into Zhongyou821 to create OE materials with Basta resistance, as described previously ( Weng et al, 2014 ). The rapeseed and tobacco seeds were soaked in Petri dishes and stratified at 4°C for 2–3 days.…”

Section: Methodsmentioning

confidence: 99%

Rapeseed (Brassica napus) Mitogen-Activated Protein Kinase 1 Enhances Shading Tolerance by Regulating the Photosynthesis Capability of Photosystem II

et al. 2022

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Rapeseed (Brassica napus) is the third-largest source of vegetable oil in the world with an edible, medicinal, and ornamental value. However, insufficient light or high planting density directly affects its growth, development, yield, and quality. Mitogen-activated protein kinases (MAPKs) are serine/threonine protein kinases that play key roles in regulating the responses to biotic and abiotic stresses in plants. In this study, we found that the promoter of BnaMAPK1 contained several light-responsive elements (including the AT1-motif, G-Box, and TCT-motif), consistent with its shading stress-induced upregulation. Compared with the wild type under shading stress, BnaMAPK1-overexpressing plants showed higher light capture efficiency and carbon assimilation capacity, enhancing their shading tolerance. Using RNA sequencing, we systematically investigated the function of BnaMAPK1 in shading stress on photosynthetic structure, Calvin cycle, and light-driven electron transport. Notably, numerous genes encoding light-harvesting chlorophyll a/b-binding proteins (BnaLHCBs) in photosystem II-light-harvesting complex (LHC) II supercomplex were significantly downregulated in the BnaMAPK1-overexpressing lines relative to the wild type under shading stress. Combining RNA sequencing and yeast library screening, a candidate interaction partner of BnaMAPK1 regulating in shading stress, BnaLHCB3, was obtained. Moreover, yeast two-hybrid and split-luciferase complementation assays confirmed the physical interaction relationship between BnaLHCB3 and BnaMAPK1, suggesting that BnaMAPK1 may involve in stabilizing the photosystem II–LHC II supercomplex. Taken together, our results demonstrate that BnaMAPK1 positively regulates photosynthesis capability to respond to shading stress in rapeseed, possibly by controlling antenna proteins complex in photosystem II, and could provide valuable information for further breeding for rapeseed stress tolerance.

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Over-Expression of BnMAPK1 in Brassica napus Enhances Tolerance to Drought Stress

Cited by 19 publications

References 39 publications

Genome-Wide Identification and Analysis of MKK and MAPK Gene Families in Brassica Species and Response to Stress in Brassica napus

Genome-Wide Identification and Analysis of MKK and MAPK Gene Families in Brassica Species and Response to Stress in Brassica napus

Characterization of the gene expression profile response to drought stress in Haloxylon Bunge using PacBio SMRT and Illumina sequencing

Rapeseed (Brassica napus) Mitogen-Activated Protein Kinase 1 Enhances Shading Tolerance by Regulating the Photosynthesis Capability of Photosystem II

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