Over-expression of a vacuolar Na+/H+ antiporter gene improves salt tolerance in an upland rice

Chen, Hui; An, Rui; Tang, Jiang-Hua; Cui, Xin; Hao, Fushun; Chen, Jia; Wang, Xuechen

doi:10.1007/s11032-006-9048-8

Cited by 127 publications

(80 citation statements)

References 28 publications

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“…Subsequent studies found greater accumulation of both Na + and K + in shoots of transgenic plants overexpressing NHX-like proteins. 23,27 By contrast, several reports failed to find a significant correlation between increased salt tolerance and enhanced accumulation of Na + , 26,[28][29][30] or described greater K + contents, rather than of Na + , in tissues of the transgenic plants. 18,24,25 It should be noted that these reports relied on determinations of total Na + and K + contents in lieu of direct measurements of vacuolar content as in Leidi et al 13 Therefore, although Na + /H + exchange at the tonoplast is generally agreed to play a major role in the salt tolerance of plants, no conclusive evidence has been presented to substantiate that NHX-like antiporters are crucial in this process.…”

Section: Mini-reviewmentioning

confidence: 87%

How do vacuolar NHX exchangers function in plant salt tolerance?

Jiang

Leidi

Pardo

2010

Plant Signaling & Behavior

138

113

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Section: Mini-reviewmentioning

confidence: 87%

How do vacuolar NHX exchangers function in plant salt tolerance?

Jiang

Leidi

Pardo

2010

Plant Signaling & Behavior

138

113

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“…For instance, Arabidopsis plants overexpressing the vacuolar Na + /H + antiporter AtNHX1 were able to thrive on NaCl concentrations as high as 200 mM, exhibiting far superior growth than the wild type while containing about 30 % more Na + (Apse et al 1999). Similarly, overexpression of OsNHX1 in upland rice resulted in growth significantly higher than that of wild type, while at the same time displaying both increases in shoot Na + and decreases in root and shoot K + (Chen et al 2007). Overexpression of the HvHKT2;1 gene (whose product is implicated in sodium influx-see above) in young barley plants resulted in improved growth despite increased Na + uptake, translocation, and shoot accumulation (Mian et al 2011).…”

Section: Sodium Toxicitymentioning

confidence: 99%

Sodium as nutrient and toxicant

et al. 2013

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Background Sodium (Na + ) is one of the most intensely researched ions in plant biology and has attained a reputation for its toxic qualities. Following the principle of Theophrastus Bombastus von Hohenheim (Paracelsus), Na + is, however, beneficial to many species at lower levels of supply, and in some, such as certain C4 species, indeed essential. Scope Here, we review the ion's divergent roles as a nutrient and toxicant, focusing on growth responses, membrane transport, stomatal function, and paradigms of ion accumulation and sequestration. We examine connections between the nutritional and toxic roles throughout, and place special emphasis on the relationship of Na + to plant potassium (K + ) relations and homeostasis. Conclusions Our review investigates intriguing connections and disconnections between Na + nutrition and toxicity, and concludes that several leading paradigms in the field, such as on the roles of Na + influx and tissue accumulation or the cytosolic K + /Na + ratio in the development of toxicity, are currently insufficiently substantiated and require a new, critical approach.

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“…After Na + uptake, a fraction of Na + taken up is sequestered into the vacuoles of both root and shoot cells by AtNHX1, a vacuolar Na + , K + /H + exchanger in Arabidopsis (Apse et al, 1999(Apse et al, , 2003Leidi et al, 2010). Its homolog in rice has also been implicated in salt tolerance, although the exact role is not well understood (Chen et al, 2007).…”

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confidence: 99%

A Magnesium Transporter OsMGT1 Plays a Critical Role in Salt Tolerance in Rice

et al. 2017

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Salt stress is one of the major factors limiting rice (Oryza sativa) production globally. Although several transporters involved in salt tolerance have been identified in rice, the mechanisms regulating their transport activity are still poorly understood. Here, we show evidence that a rice Mg transporter OsMGT1 is required for salt tolerance probably by regulating transport activity of OsHKT1;5, a key transporter for the removal of Na + from the xylem sap at the root mature zone. Knockout of OsMGT1 did not affect total Na uptake, but increased Na concentration in the shoots and xylem sap, resulting in a significant increase in salt sensitivity at low external Mg 2+ concentration (20-200 mM). However, such differences were abolished at a higher Mg 2+ concentration (2 mM), although the total Na uptake was not altered. OsMGT1 was expressed in both the roots and shoots, but only that in the roots was moderately up-regulated by salt stress. Spatial expression analysis revealed that OsMGT1 was expressed in all root cells of the root tips but was highly expressed in the pericycle of root mature zone. OsMGT1 was also expressed in the phloem region of basal node, leaf blade, and sheath. When expressed in Xenopus laevis oocytes, the transport activity of OsHKT1;5 was enhanced by elevating external Mg 2+ concentration. Furthermore, knockout of OsHKT1;5 in osmgt1 mutant background did not further increase its salt sensitivity. Taken together, our results suggest that Mg 2+ transported by OsMGT1 in the root mature zone is required for enhancing OsHKT1;5 activity, thereby restricting Na accumulation to the shoots.

show abstract

Over-expression of a vacuolar Na+/H+ antiporter gene improves salt tolerance in an upland rice

Cited by 127 publications

References 28 publications

How do vacuolar NHX exchangers function in plant salt tolerance?

How do vacuolar NHX exchangers function in plant salt tolerance?

Sodium as nutrient and toxicant

A Magnesium Transporter OsMGT1 Plays a Critical Role in Salt Tolerance in Rice

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