Over-expression of a small heat shock protein, sHSP17.7, confers both heat tolerance and UV-B resistance to rice plants

Murakami, Tetuhide H.; Matsuba, Shuichi; Funatsuki, Hideyuki; Kawaguchi, Kenichi; Saruyama, Haruo; Tanida, Masatoshi; Satō, Yutaka

doi:10.1023/b:molb.0000018764.30795.c1

Cited by 161 publications

(98 citation statements)

References 48 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Previous studies on Oryza sativa heat shock protein (OsHSP) genes showed that they exhibited diverse expression in different organs and enhanced the tolerance of transgenic plants to various stresses, including abiotic stress (Sun et al 2002;Zou et al 2009). In addition, sHSPs are known to function in pollen development and seed germination, as well as resistance to UV damage, enhancement of cell survival against both thermal extremes (50℃ and 4℃) and seed and root development Murakami et al 2004;Zou et al 2009). Expression of the Oshsp16.9 gene transcript was observed in different parts of the plant, including the root, stem, leaf, spike and seed.…”

Section: Discussionmentioning

confidence: 99%

“…It is believed that this diversification and abundance of the sHsps in plants is a reflection of the adaptation of the plant to heat stress (Waters et al 1996). Earlier studies demonstrated that OsHsps can act as a protein chaperone, protecting pollen mother cells and tapetal cells against extreme heat (Murakami et al 2004;Zou et al 2009). To examine further its functioning and molecular mechanism under abiotic stresses, we transformed Oshsp16.9 into rice as our model system.…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Overexpression of Oshsp16.9 Gene Encoding Small Heat Shock Protein Enhances Tolerance to Abiotic Stresses in Rice

Jung

Nou

Kang

2014

Plant Breed. Biotech.

View full text Add to dashboard Cite

This is an Open-Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/3.0) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited. ABSTRACT Plants have adapted the ability to respond to various abiotic stresses such as high salinity, osmotic stress, high and low temperatures, and drought in order to survive. Small heat shock proteins (sHsps) play important and extensive roles in plant defenses against abiotic stresses. Herein, we cloned an sHsp gene from the rice, which we named Oshsp16.9 based on the molecular weight of the protein.Real-time PCR analysis showed that expression of the Oshsp16.9 gene was rapidly and strongly induced by stresses including high-salinity (250 mM NaCl), osmotic stress (300 mM mannitol), 100 μM ABA, cold (4°C) and heat (45°C). Subcellular localization assay indicated that Oshsp16.9 was localized specifically in the cytoplasm. In addition, overexpression of Oshsp16.9 in rice conferred tolerance of transgenic plants to salt and drought stress. Taken together, these results suggest that the Oshsp16.9 gene is an important determinant of stress response in plants.

show abstract

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Overexpression of Oshsp16.9 Gene Encoding Small Heat Shock Protein Enhances Tolerance to Abiotic Stresses in Rice

Jung

Nou

Kang

2014

Plant Breed. Biotech.

View full text Add to dashboard Cite

show abstract

“…mays (Lund et al, 2001), Agrostis stolonifera L. (Heckathorn et al, 2002), Oryza sativa L. (Murakami et al, 2004;Sarkar et al, 2009), Rosa chinensis Jacq. (Jiang et al, 2009), Prunus salicina Lindl.…”

Section: Discussionmentioning

confidence: 99%

Effects of hypoxia storage on gene transcript accumulation during tomato fruit ripening

Pegoraro

Santos

Krüger

et al. 2012

Braz. J. Plant Physiol.

View full text Add to dashboard Cite

Tomato (Solanum lycopersicum L.) is a climacteric fruit, i.e., during ripening an increase in ethylene synthesis and high rate of respiration are observed. Low oxygen levels might inhibit or block ethylene biosynthesis and therefore retard the ripening process. Despite commercial applications of low oxygen treatments, the precise mode of action of low oxygen in fruit tissues and ripening is not well understood. In order to delineate the molecular responses to low oxygen stress in fruits, hypoxia-responsive tomato genes encoding heat shock factors, heat shock proteins, and enzymes involved in fermentation and ethylene synthesis pathways were analyzed. In this study, tomato fruit stored under hypoxia conditions showed that HSP17.7 and HSP21 genes were highly induced by low oxygen level, indicating their primary role in maintaining cellular homeostasis after this stress.

show abstract

“…OsHSP70 and OsHSP71.1 were highly expressed during seed imbibition (Zou et al, 2009). Over expression of Oryza sativa sHSP 17.7 (Murakami et al, 2004) and sHSP 18.0 , also increases the degree of resistance to UV damage in transgenic rice and Escherichia coli respectively. sHSPs of Chestnut (Castanea sativa) CsHSP 17.5 belonging to cystolic class I, enhance the cell survival against both thermal extremes (50 and 4°C) (Soto et al, 1999).…”

Section: Shsp Genes Expressionmentioning

confidence: 99%

An overview on the small heat shock proteins

Mahmood¹,

Safdar²,

Abbasi³

et al. 2010

Afr. J. Biotechnol.

View full text Add to dashboard Cite

In the last 25 years, a huge amount of literature has been accumulated describing the cell's response to different kinds of environmental stress conditions, such as high temperatures, altered pH, exposure of the cell to toxins, starvation, oxygen, and water deprivation, among others. Heat shock proteins (HSPs) are one of the main expressed products of the cell in response to stresses. HSPs can be classified into six structurally conserved classes according to their molecular weight namely, HSP100, HSP90, HSP70, HSP60, small heat shock proteins (sHSPs) and ubiquitin (8.5 kDa). In eukaryotes, different heat shock genes are expressed uncoordinatedly, whereas in prokaryote, heat shock genes form a regulon and appear simultaneously. sHSPs are associated with nuclei, cytoskeleton and membranes. They bind partially to denatured proteins, preventing irreversible protein aggregation during stress. In animals, only one sHSP gene has been located in yeast cells, ten in mammalian, two in birds and four genes have been found in Drosophila. However, in plants more than 20 sHSPs have been reported and they can be divided into 6 classes, of which, 3 classes (CI, CII and CIII) are in the cytosole or in the nucleus and the other three (CIV, CV and CVI) in the plastids, endoplasmic reticulum and mitochondria. Mitochondrial and chloroplast sHSPs protect electron transport chain. During development in animals, sHSP genes are normally regulated at late neurula and early tailbud stage and in plants during pollen development, seed maturation, seed imbibition and germination. Transcriptional regulation of sHSPs depends on particular activation of heat shock factors (HSF) which recognize the highly conserved heat-shock elements (HSEs). After the heat stress has been released, the sHSPs are quite stable, suggesting that sHSPs may be important for recovery as well.

show abstract

Over-expression of a small heat shock protein, sHSP17.7, confers both heat tolerance and UV-B resistance to rice plants

Cited by 161 publications

References 48 publications

Overexpression of Oshsp16.9 Gene Encoding Small Heat Shock Protein Enhances Tolerance to Abiotic Stresses in Rice

Overexpression of Oshsp16.9 Gene Encoding Small Heat Shock Protein Enhances Tolerance to Abiotic Stresses in Rice

Effects of hypoxia storage on gene transcript accumulation during tomato fruit ripening

An overview on the small heat shock proteins

Contact Info

Product

Resources

About