Over expression of a Chitinase 2 gene from Chinese Wild Strawberry improves resistance to anthracnose disease in transgenic Arabidopsis thaliana

Wen, Zhi‐Hong; Bai, Jinhui; Wang, Lin; Yao, Liping; Ahmad, Bilal; Hanif, Muhammad; Chen, Qing‐Xi

doi:10.1007/s11816-020-00648-z

Cited by 11 publications

(6 citation statements)

References 58 publications

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“…We found ten genes commonly expressed or marked between two datasets by comparing ChIP-Seq and RNA-Seq analyses of DT, DTHT, and HT stresses. Among these, the essential genes that triggered in response to abiotic and biotic stresses with known functions were: 1) Aldolase-type TIM barrel family protein, also known as pyridoxal 5'-phosphate synthase pdxS subunit (pdxS and pdx1), which is involved in protein binding and cofactor biosynthesis that plays a role in protecting cellular membranes from lipid peroxidation [ 84 ]; 2) Condensin, subunit H, is an essential gene required for chromosome stability, condensation, and segregation [ 85 ]; 3) NB-ARC domain-containing or LRR containing proteins that are known to be involved in resistance to plant stresses [ 86 ]; 4) Pectin acetylesterase family protein or Notum-related proteins that play a role in structural stability, binding, and catalytic activity [ 87 ]; 5) Putative Mg-protoporphyrin IX chelatase subunit CHLH that is involved in chlorophyll biosynthesis [ 88 ]; 6) Receptor lectin kinases that are involved in plant development and stresses [ 89 ]; and 7) rRNA N-glycosylase/N-glycosidase enzymes that are induced during abiotic stresses [ 90 ] (Table 9 ).…”

Section: Discussionmentioning

confidence: 99%

Genome-wide profiling of histone (H3) lysine 4 (K4) tri-methylation (me3) under drought, heat, and combined stresses in switchgrass

Ayyappan,

Sripathi,

Xie

et al. 2024

BMC Genomics

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Background Switchgrass (Panicum virgatum L.) is a warm-season perennial (C4) grass identified as an important biofuel crop in the United States. It is well adapted to the marginal environment where heat and moisture stresses predominantly affect crop growth. However, the underlying molecular mechanisms associated with heat and drought stress tolerance still need to be fully understood in switchgrass. The methylation of H3K4 is often associated with transcriptional activation of genes, including stress-responsive. Therefore, this study aimed to analyze genome-wide histone H3K4-tri-methylation in switchgrass under heat, drought, and combined stress. Results In total, ~ 1.3 million H3K4me3 peaks were identified in this study using SICER. Among them, 7,342; 6,510; and 8,536 peaks responded under drought (DT), drought and heat (DTHT), and heat (HT) stresses, respectively. Most DT and DTHT peaks spanned 0 to + 2000 bases from the transcription start site [TSS]. By comparing differentially marked peaks with RNA-Seq data, we identified peaks associated with genes: 155 DT-responsive peaks with 118 DT-responsive genes, 121 DTHT-responsive peaks with 110 DTHT-responsive genes, and 175 HT-responsive peaks with 136 HT-responsive genes. We have identified various transcription factors involved in DT, DTHT, and HT stresses. Gene Ontology analysis using the AgriGO revealed that most genes belonged to biological processes. Most annotated peaks belonged to metabolite interconversion, RNA metabolism, transporter, protein modifying, defense/immunity, membrane traffic protein, transmembrane signal receptor, and transcriptional regulator protein families. Further, we identified significant peaks associated with TFs, hormones, signaling, fatty acid and carbohydrate metabolism, and secondary metabolites. qRT-PCR analysis revealed the relative expressions of six abiotic stress-responsive genes (transketolase, chromatin remodeling factor-CDH3, fatty-acid desaturase A, transmembrane protein 14C, beta-amylase 1, and integrase-type DNA binding protein genes) that were significantly (P < 0.05) marked during drought, heat, and combined stresses by comparing stress-induced against un-stressed and input controls. Conclusion Our study provides a comprehensive and reproducible epigenomic analysis of drought, heat, and combined stress responses in switchgrass. Significant enrichment of H3K4me3 peaks downstream of the TSS of protein-coding genes was observed. In addition, the cost-effective experimental design, modified ChIP-Seq approach, and analyses presented here can serve as a prototype for other non-model plant species for conducting stress studies.

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Section: Discussionmentioning

confidence: 99%

Genome-wide profiling of histone (H3) lysine 4 (K4) tri-methylation (me3) under drought, heat, and combined stresses in switchgrass

Ayyappan,

Sripathi,

Xie

et al. 2024

BMC Genomics

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“…defense responses to B. cinerea, Colletotrichum higginsianum, and R. solani, respectively (Kishimoto et al, 2004;Richa et al, 2017;Wen et al, 2020) (Figure 3A). There were more studies that confirmed the function of GH19 plant chitinases, and 27 functionally validated plant chitinases from apple, barley, cocoa, common bean, cucumber, maize, pepper, rice, and wheat were included in the phylogenetic analysis together with 12 GH19 soybean chitinases.…”

Section: Phylogenetic Analysis For Soybean Chitinases In Defense Resp...mentioning

confidence: 99%

Characterization of soybean chitinase genes induced by rhizobacteria involved in the defense against Fusarium oxysporum

Chen,

Sang,

Chilvers

et al. 2024

Front. Plant Sci.

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Rhizobacteria are capable of inducing defense responses via the expression of pathogenesis-related proteins (PR-proteins) such as chitinases, and many studies have validated the functions of plant chitinases in defense responses. Soybean (Glycine max) is an economically important crop worldwide, but the functional validation of soybean chitinase in defense responses remains limited. In this study, genome-wide characterization of soybean chitinases was conducted, and the defense contribution of three chitinases (GmChi01, GmChi02, or GmChi16) was validated in Arabidopsis transgenic lines against the soil-borne pathogen Fusarium oxysporum. Compared to the Arabidopsis Col-0 and empty vector controls, the transgenic lines with GmChi02 or GmChi16 exhibited fewer chlorosis symptoms and wilting. While GmChi02 and GmChi16 enhanced defense to F. oxysporum, GmChi02 was the only one significantly induced by Burkholderia ambifaria. The observation indicated that plant chitinases may be induced by different rhizobacteria for defense responses. The survey of 37 soybean chitinase gene expressions in response to six rhizobacteria observed diverse inducibility, where only 10 genes were significantly upregulated by at least one rhizobacterium and 9 genes did not respond to any of the rhizobacteria. Motif analysis on soybean promoters further identified not only consensus but also rhizobacterium-specific transcription factor-binding sites for the inducible chitinase genes. Collectively, these results confirmed the involvement of GmChi02 and GmChi16 in defense enhancement and highlighted the diverse inducibility of 37 soybean chitinases encountering F. oxysporum and six rhizobacteria.

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“…Current studies have shown an outstanding reduction in contagion of beans and cotton plants by soil fungi Sclerotium rolfsii and Rhizoctonia solani on the presence of the overproducing Escherichia coli strain of ChiA [ 141 ]. In addition, transgenic Arabidopsis thaliana containing the Chitinase 2 gene from Chinese Wild Strawberry improves resistance to anthracnose disease [ 142 ].…”

Section: Chitin and Chitosan Degradation Enzymesmentioning

confidence: 99%

“…Contrastingly, it has been suggested that GH19 chitinase genes have been transferred to nematodes and arthropods by purple bacteria and Actinobacteria who have procured it from plants [ 142 ]. Recently, only one GH23 chitinase is found in bacteria, especially it has been isolated from Ralstonia sp.…”

Section: Chitin and Chitosan Degradation Enzymesmentioning

confidence: 99%

Chemical Proprieties of Biopolymers (Chitin/Chitosan) and Their Synergic Effects with Endophytic Bacillus Species: Unlimited Applications in Agriculture

et al. 2021

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Over the past decade, reckless usage of synthetic pesticides and fertilizers in agriculture has made the environment and human health progressively vulnerable. This setting leads to the pursuit of other environmentally friendly interventions. Amongst the suggested solutions, the use of chitin and chitosan came about, whether alone or in combination with endophytic bacterial strains. In the framework of this research, we reported an assortment of studies on the physico-chemical properties and potential applications in the agricultural field of two biopolymers extracted from shrimp shells (chitin and chitosan), in addition to their uses as biofertilizers and biostimulators in combination with bacterial strains of the genus Bacillus sp. (having biochemical and enzymatic properties).

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Over expression of a Chitinase 2 gene from Chinese Wild Strawberry improves resistance to anthracnose disease in transgenic Arabidopsis thaliana

Cited by 11 publications

References 58 publications

Genome-wide profiling of histone (H3) lysine 4 (K4) tri-methylation (me3) under drought, heat, and combined stresses in switchgrass

Genome-wide profiling of histone (H3) lysine 4 (K4) tri-methylation (me3) under drought, heat, and combined stresses in switchgrass

Characterization of soybean chitinase genes induced by rhizobacteria involved in the defense against Fusarium oxysporum

Chemical Proprieties of Biopolymers (Chitin/Chitosan) and Their Synergic Effects with Endophytic Bacillus Species: Unlimited Applications in Agriculture

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