Over-Expression of a 14-3-3 Protein From Foxtail Millet Improves Plant Tolerance to Salinity Stress in Arabidopsis thaliana

Liu, Jiaming; Jiang, Chengyao; Lu, Kang; Zhang, Hongchang; Song, Yu; Zou, Zhirong; Zheng, Wenbin

doi:10.3389/fpls.2020.00449

Cited by 19 publications

(16 citation statements)

References 54 publications

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“…Therefore, they have great effects on many plant biological progresses. In recent years, 14-3-3 families have been identified and studied in many plant species [16,34,35]. To date, although research on 14-3-3 family members in G. hirsutum has been performed, it mainly focused on the roles of 14-3-3 in fiber development and stress responses [24][25][26][27][28].…”

Section: Discussionmentioning

confidence: 99%

“…The cotton 14-3-3 genes appear to function in fiber cell initiation and elongation, salt and drought stress signaling, and Verticillium dahlia resistance [24][25][26][27][28]. At present, the 14-3-3 genes of several plants have been identified, such as Arabidopsis [29][30][31], rice [15,32], Medicago truncatula [33], Phyllostachys violascens [19], soybean [34], and foxtail millet (Setaria italica) [35]. However, systematic investigations of 14-3-3 family members using genome sequences and of their functions in flowering have not been reported for G. hirsutum.…”

Section: Introductionmentioning

confidence: 99%

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Roles of the 14-3-3 gene family in cotton flowering

Sang

Liu

et al. 2021

BMC Plant Biol

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Background In plants, 14-3-3 proteins, also called GENERAL REGULATORY FACTORs (GRFs), encoded by a large multigene family, are involved in protein–protein interactions and play crucial roles in various physiological processes. No genome-wide analysis of the GRF gene family has been performed in cotton, and their functions in flowering are largely unknown. Results In this study, 17, 17, 31, and 17 GRF genes were identified in Gossypium herbaceum, G. arboreum, G. hirsutum, and G. raimondii, respectively, by genome-wide analyses and were designated as GheGRFs, GaGRFs, GhGRFs, and GrGRFs, respectively. A phylogenetic analysis revealed that these proteins were divided into ε and non-ε groups. Gene structural, motif composition, synteny, and duplicated gene analyses of the identified GRF genes provided insights into the evolution of this family in cotton. GhGRF genes exhibited diverse expression patterns in different tissues. Yeast two-hybrid and bimolecular fluorescence complementation assays showed that the GhGRFs interacted with the cotton FLOWERING LOCUS T homologue GhFT in the cytoplasm and nucleus, while they interacted with the basic leucine zipper transcription factor GhFD only in the nucleus. Virus-induced gene silencing in G. hirsutum and transgenic studies in Arabidopsis demonstrated that GhGRF3/6/9/15 repressed flowering and that GhGRF14 promoted flowering. Conclusions Here, 82 GRF genes were identified in cotton, and their gene and protein features, classification, evolution, and expression patterns were comprehensively and systematically investigated. The GhGRF3/6/9/15 interacted with GhFT and GhFD to form florigen activation complexs that inhibited flowering. However, GhGRF14 interacted with GhFT and GhFD to form florigen activation complex that promoted flowering. The results provide a foundation for further studies on the regulatory mechanisms of flowering.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Roles of the 14-3-3 gene family in cotton flowering

Sang

Liu

et al. 2021

BMC Plant Biol

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show abstract

“…Therefore, they have great effects on many plant biological progresses. In recent years, 14-3-3 families have been identi ed and studied in many plant species [16,34,35]. To date, although research on 14-3-3 family members in G. hirsutum has been performed, it mainly focused on the roles of 14-3-3 in ber development and stress responses [24][25][26][27][28].…”

Section: Discussionmentioning

confidence: 99%

“…The cotton 14-3-3 genes appear to function in ber cell initiation and elongation, salt and drought stress signaling, and Verticillium dahlia resistance [24][25][26][27][28]. At present, the 14-3-3 genes of several plants have been identi ed, such as Arabidopsis [29][30][31], rice [15,32], Medicago truncatula [33], Phyllostachys violascens [19], soybean [34], and foxtail millet [35]. However, systematic investigations of 14-3-3 family members using genome sequences and of their functions in owering have not been reported for G. hirsutum.…”

Section: Introductionmentioning

confidence: 99%

Roles of the 14-3-3 Gene Family in Cotton Flowering

Sang

Liu

et al. 2021

Preprint

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Background: In plants, 14-3-3 proteins, also called GENERAL REGULATORY FACTORs (GRFs), encoded by a large multigene family, are involved in protein–protein interactions and play crucial roles in various physiological processes. No genome-wide analysis of the GRF gene family has been performed in cotton, and their functions in flowering are largely unknown.Results: In this study, 17, 17, 31, and 17 GRF genes were identified in Gossypium herbaceum, G. arboreum, G. hirsutum, and G. raimondii, respectively, by genome-wide analyses and were designated as GheGRFs, GaGRFs, GhGRFs, and GrGRFs, respectively. A phylogenetic analysis revealed that these proteins were divided into ε and non-ε groups. Gene structural, motif composition, synteny, and duplicated gene analyses of the identified GRF genes provided insights into the evolution of this family in cotton. GhGRF genes exhibited diverse expression patterns in different tissues. Yeast two-hybrid and bimolecular fluorescence complementation assays showed that the GhGRFs interacted with the cotton FLOWERING LOCUS T homologue GhFT in the cytoplasm and nucleus, while they interacted with the basic leucine zipper transcription factor GhFD only in the nucleus. Transgenic studies in Arabidopsis demonstrated that GhGRF3/6/9/15 repressed flowering and that GhGRF14 promoted flowering.Conclusions: Here, 82 GRF genes were identified in cotton, and their gene and protein features, classification, evolutionary, and expression patterns were comprehensively and systematically investigated. The GhGRFs interacted with GhFT and GhFD to form florigen activation complexes, which either promoted or inhibited flowering. The results provide a foundation for further studies on the regulatory mechanisms of flowering.

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“…The SiGRF1 gene may regulate the initiation date of flowering in plants exposed to salt stress by up-regulating the transcription level of WRKY71 to promote Flowering Locus T (FT) and LEAFY (LFY) expression to act against the inhibition by DELLAs, etc. Additionally, SiGRF1 helps to avoid salt stress by accelerating plant flowering time [63].…”

Section: E3s Participate In the Flowering Pathwaymentioning

confidence: 99%

Roles of E3 Ubiquitin Ligases in Plant Responses to Abiotic Stresses

Wang

Zhang

et al. 2022

IJMS

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Plants are frequently exposed to a variety of abiotic stresses, such as those caused by salt, drought, cold, and heat. All of these stressors can induce changes in the proteoforms, which make up the proteome of an organism. Of the many different proteoforms, protein ubiquitination has attracted a lot of attention because it is widely involved in the process of protein degradation; thus regulates many plants molecular processes, such as hormone signal transduction, to resist external stresses. Ubiquitin ligases are crucial in substrate recognition during this ubiquitin modification process. In this review, the molecular mechanisms of plant responses to abiotic stresses from the perspective of ubiquitin ligases have been described. This information is critical for a better understanding of plant molecular responses to abiotic stresses.

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Over-Expression of a 14-3-3 Protein From Foxtail Millet Improves Plant Tolerance to Salinity Stress in Arabidopsis thaliana

Cited by 19 publications

References 54 publications

Roles of the 14-3-3 gene family in cotton flowering

Roles of the 14-3-3 gene family in cotton flowering

Roles of the 14-3-3 Gene Family in Cotton Flowering

Roles of E3 Ubiquitin Ligases in Plant Responses to Abiotic Stresses

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