Over-expressed RPL34 promotes malignant proliferation of non-small cell lung cancer cells

Yang, Shaoxing; Cui, Jian; Yang, Yingshun; Liu, Zhaoping; Yan, Haiying; Tang, Chuanhao; Wang, Hong; Qin, Haifeng; Li, Xiaoyan; Li, Jianjie; Wang, Weixia; Huang, Yuqing; Gao, Hongjun

doi:10.1016/j.gene.2015.10.053

Cited by 40 publications

(39 citation statements)

References 25 publications

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“…Enhanced expression of RPL34 contributes to the cancerous transformation and development of gastric cancer in humans, while RNAi-mediated inhibition of its expression significantly reduces cell proliferation and escalates apoptosis (Liu et al 2015b). Similar RPL34 action was observed in the cells of non-small cell lung cancer (NSCLC) (Yang et al 2016a) as well as of esophageal cancer (Fan et al 2017). Likewise, RPL34 overexpression in osteosarcoma promotes proliferation of malignant cells.…”

Section: A Nucleolus As a Support Of Cancer Cellssupporting

confidence: 59%

The nucleolus, an ally, and an enemy of cancer cells

Stępiński

2018

Histochem Cell Biol

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The rates of ribosome production by a nucleolus and of protein biosynthesis by ribosomes are tightly correlated with the rate of cell growth and proliferation. All these processes must be matched and appropriately regulated to provide optimal cell functioning. Deregulation of certain factors, including oncogenes, controlling these processes, especially ribosome biosynthesis, can lead to cell transformation. Cancer cells are characterized by intense ribosome biosynthesis which is advantageous for their growth and proliferation. On the other hand, this feature can be engaged as an anticancer strategy. Numerous nucleolar factors such as nucleolar and ribosomal proteins as well as different RNAs, in addition to their role in ribosome biosynthesis, have other functions, including those associated with cancer biology. Some of them can contribute to cell transformation and cancer development. Others, under stress evoked by different factors which often hamper function of nucleoli and thus induce nucleolar/ribosomal stress, can participate in combating cancer cells. In this sense, intentional application of therapeutic agents affecting ribosome biosynthesis can cause either release of these molecules from nucleoli or their de novo biosynthesis to mediate the activation of pathways leading to elimination of harmful cells. This review underlines the role of a nucleolus not only as a ribosome constituting apparatus but also as a hub of both positive and negative control of cancer development. The article is mainly based on original papers concerning mechanisms in which the nucleolus is implicated directly or indirectly in processes associated with neoplasia.

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Section: A Nucleolus As a Support Of Cancer Cellssupporting

confidence: 59%

The nucleolus, an ally, and an enemy of cancer cells

Stępiński

2018

Histochem Cell Biol

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“…However, in recent years, an increasing number of researches have associated RPL34 with the extraribosomal functions related to cellular proliferation31. More importantly, several reports have showed that RPL34 plays a critical role in the proliferation, cell cycle progression and apoptosis of malignant cells2526. Nonetheless, its role in OS has been poorly characterized.…”

Section: Discussionmentioning

confidence: 99%

“…showed that RPL34 was highly expressed in gastric cancer cell lines, and that knockdown of RPL34 in SGC-7901 cells significantly suppressed cell proliferation, induced apoptosis and arrested cells in S phase. Yang et al 26. found that RPL34 expression was up-regulated in non-small cell lung cancer (NSCLC) tissues compared with adjacent tissues.…”

mentioning

confidence: 99%

Highly expressed ribosomal protein L34 indicates poor prognosis in osteosarcoma and its knockdown suppresses osteosarcoma proliferation probably through translational control

Luo

Zhao

Fowdur

et al. 2016

Sci Rep

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Osteosarcoma has devastating health implications on children and adolescents. However, due to its low incidence and high tumor heterogeneity, it is hard to achieve any further improvements in therapy and overall survival. Ribosomal protein L34 (RPL34) has been increasingly recognized to promote the proliferation of malignant cells, but its role in osteosarcoma has not been investigated. In this study, real-time quantitative PCR (RT-qPCR) and immunohistochemistry revealed that RPL34 was highly expressed in osteosarcoma tissues when compared to adjacent tissues and normal bone tissues. Survival analysis showed that high expression of RPL34 predicted a poor prognosis for osteosarcoma patients. Knockdown of RPL34 in Saos-2 cells via lentivirus-mediated small interfering RNA (siRNA) significantly inhibited cell proliferation, induced cell apoptosis and G2/M phase arrest. Moreover, screening of transcription factors using University of California Santa Cruz (UCSC) Genome Browser, protein-protein interaction (PPI) network analysis, Gene Ontology (GO) and pathway enrichment analysis revealed that MYC participates in the transcriptional regulation of RPL34, which interacts with the subunits of eukaryotic translation initiation factor 3 (eIF3) and probably involves the translational control of growth-promoting proteins. Our findings suggest that RPL34 plays an important role in the proliferation of osteosarcoma cells.

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“…RPL34 is a rarely studied ribosomal protein and most previous studies mainly focused on its expression and functions in mosquitoes and plants [18, 19]. Recently, two reports revealed RPL34 promoted cell proliferation in non-small cell lung and gastric cancers [14, 15]; however, the roles for RPL34 in cell malignances and the mechanisms of its dysregulation remain unexplored in PCs.…”

Section: Discussionmentioning

confidence: 99%

“…In addition to functioning as a ribosomal protein, RPL34 has been reported to play an important role in other cellular processes. RPL34 regulates cell cycle transition by inhibiting Cdk4 and Cdk5 in HeLa cells [13], and over-expression of RPL34 promotes cell proliferation in human non-small cell lung cancer and gastric cancer [14, 15]. However, the biological function and clinical significance of RPL34 in human PC remains largely unknown.…”

Section: Introductionmentioning

confidence: 99%

Ribosomal protein L34 promotes the proliferation, invasion and metastasis of pancreatic cancer cells

et al. 2016

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Ribosomal proteins (RPs) are the main components of ribosomes and participate in the self-assembly of ribosomes and protein synthesis. Recent advance has shown that RPs play important roles in the tumorigenesis and drug resistance of various cancers. However, the expression status and function of RPL34 in pancreatic cancer (PC) remains unclear. In this study, we find that RPL34 is overexpressed in PC tissues and cell lines, which is correlated with decreased methylation of its promoter. Knockdown of RPL34 effectively suppresses the proliferation, colony formation, migration and drug-resistance of PC cells, which are accompanied by cell cycle arrest at the G2 phase and induction of apoptosis. In vivo assays demonstrate that RPL34 silencing inhibits PC tumor growth and metastasis. Moreover, gene expression profiling revealed that RPL34 silencing results in alteration of the MAPK and p53 signaling pathways. Clinically, our data indicate a positive association of RPL34 expression with tumor stage and metastasis in PCs. We revealed that RPL34 acts as a potential onco-protein in PC, and RPL34 may be a promising biomarker for prognosis prediction and a potential target for the treatment of PC.

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Over-expressed RPL34 promotes malignant proliferation of non-small cell lung cancer cells

Cited by 40 publications

References 25 publications

The nucleolus, an ally, and an enemy of cancer cells

The nucleolus, an ally, and an enemy of cancer cells

Highly expressed ribosomal protein L34 indicates poor prognosis in osteosarcoma and its knockdown suppresses osteosarcoma proliferation probably through translational control

Ribosomal protein L34 promotes the proliferation, invasion and metastasis of pancreatic cancer cells

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