Over‐accumulation of abscisic acid in transgenic tomato plants increases the risk of hydraulic failure

Lamarque, Laurent J.; Delzon, Sylvain; Toups, Haley S.; Gravel, Anne‐Isabelle; Corso, Déborah; Badel, Éric; Burlett, Régis; Charrier, Guillaume; Cochard, Hervé; Jansen, Steven; King, Andrew; Torres‐Ruiz, José M.; Pouzoulet, Jérôme; Cramer, Grant R.; Thompson, Andrew J.; Gambetta, Grégory A.

doi:10.1111/pce.13703

Cited by 29 publications

(27 citation statements)

References 111 publications

(156 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…From 2017 onwards, we developed a new dynamic version of this model, based on a plant segmentation in different organs and implemented in the C programming language. This model has already been used in a number of recent studies (Martin-StPaul et al 2017Scoffoni et al 2018;Duursma et al 2019;Cochard 2020a;Brodribb et al 2019;Brodribb et al 2020;Dayer et al 2020;Lamarque et al 2020;Lopez et al 2021), but never formally described as here.…”

Section: Introductionmentioning

confidence: 99%

SurEau: a mechanistic model of plant water relations under extreme drought

Cochard

Pimont

Ruffault

et al. 2021

Annals of Forest Science

Self Cite

View full text Add to dashboard Cite

Key message A new process-based model,SurEau, is described. It predicts the risk of xylem hydraulic failure under drought. Context The increase in drought intensity due to climate change will accentuate the risk of tree mortality. But very few process-based models are currently able to predict this mortality risk. Aims We describe the operating principle of a new mechanistic model SurEau that computes the water balance, water relations, and hydraulics of a plant under extreme drought. Methods SurEau is based on the formalization of key physiological processes of plant response to water stress. The hydraulic and hydric functioning of the plant is at the core of this model, which focuses on both water flows (i.e., hydraulic) and water pools (i.e., hydric) using variable hydraulic conductances. The model considers the elementary flow of water from the soil to the atmosphere through different plant organs that are described by their symplasmic and apoplasmic compartments. For each organ, the symplasm is described by a pressure-volume curve and the apoplasm by its vulnerability curve to cavitation. The model is evaluated on mature oak trees exposed to water stress. Results On the tested oak trees, the model captures well the observed soil water balance, water relations, and level of embolism. A sensitivity analysis reveals that the level of embolism is strongly determined by air VPD and key physiological traits such as cuticular transpiration, resistance to cavitation, and leaf area. Conclusion The process-based SurEau model offers new opportunities to evaluate how different species or genotypes will respond to future climatic conditions.

show abstract

Section: Introductionmentioning

confidence: 99%

SurEau: a mechanistic model of plant water relations under extreme drought

Cochard

Pimont

Ruffault

et al. 2021

Annals of Forest Science

Self Cite

View full text Add to dashboard Cite

show abstract

“…With global warming, fresh water resources are increasingly scarce (Shahsavari, Karandish, & Haghighatjou, 2019), and the reduction of available water resources is a significant challenge for agricultural development. Increasing plant water‐use efficiency (WUE) is an effective strategy to improve drought resistance (Guo et al, 2019; Jiang et al, 2019; Lamarque et al, 2020; Weng, Yoo, Gosney, Hasegawa, & Mickelbart, 2012; Yoo et al, 2010). Stomata control gas exchange between plants and the environment (Hetherington & Woodward, 2003).…”

Section: Introductionmentioning

confidence: 99%

SlTLFP8 reduces water loss to improve water‐use efficiency by modulating cell size and stomatal density via endoreduplication

Zhang

Liu

et al. 2020

Plant Cell & Environment

View full text Add to dashboard Cite

Improving plant water-use efficiency (WUE) is important to plant survival and crop yield in the context of water limitation. In this study, SlTLFP8 (Tubby-like F-box protein 8) was identified as an osmotic-induced gene in tomato. Transgenic tomato with up-regulated expression of SlTLFP8 showed enhanced water-deficient resistance, whereas knockout mutants generated by CRISPR/Cas9 were more sensitive to water deficit. SlTLFP8 overexpression significantly enhanced WUE by suppressing transpiration under both water-sufficient and water-deficient conditions. Further study showed that overexpressing SlTLFP8 significantly increased leaf epidermal cell size and thereby decreased stomatal density 10-20%, conversely SlTLFP8 knockout resulted in decreased cell size and thereby increased stomatal density 20-50%. SlTLFP8 overexpression and knockout modulated ploidy levels in leaf cells. Changes in expression of cell cycle related genes also indicated that SlTLFP8 affected cell size and stomatal density through endocycle transition. Despite changes in stomata density and transpiration, altering the expression of SlTLFP8 did not change photosynthesis. Additionally, biomass was not altered and there was little difference in fruit yield for transgenic and wild type lines under water-sufficient and water-deficient conditions. Our results demonstrate the effect of SlTLFP8 on endoreduplication and the potential of SlTLFP8 for improvement of WUE. Brief Summery This work found a new mechanism of TLP (Tubby like protein) response to waterdeficient stress. SlTLFP8, a member of TLP family, regulates water-deficient resistance by modulating water loss via affecting stomatal density. Expression of SlTLFP8 was induced by osmotic stress. Transgenic tomato lines with SlTLFP8 overexpression or SlTLFP8 knockout showed significantly differences in water-use efficiency (WUE) and water-deficient resistance. The difference of leaf water loss caused by transpiration is the main explanation of the difference in WUE and water-deficient resistance. Additionally, overexpressing SlTLFP8 significantly decreased stomatal density, while SlTLFP8 knockout resulted in increased stomatal density, and SlTLFP8 affected stomatal density through endoreduplication and altered epidermal cell size. Despite

show abstract

“…As a stress hormone, abscisic acid (ABA) is involved in the response to various adverse environmental factors, particularly to drought, through the regulation of specific signalling pathways and the modification of gene expression levels [ 7 , 8 , 9 , 10 , 11 ]. Under drought, ABA controls water loss through stomata closure, transpiration flux reduction, a decrease in water potential (ψ), and a reduction in leaf area [ 12 ].…”

Section: Introductionmentioning

confidence: 99%

Leaf Soluble Sugars and Free Amino Acids as Important Components of Abscisic Acid—Mediated Drought Response in Tomato

Živanović

Komić

Tosti

et al. 2020

Plants

View full text Add to dashboard Cite

Water deficit has a global impact on plant growth and crop yield. Climate changes are going to increase the intensity, duration and frequency of severe droughts, particularly in southern and south-eastern Europe, elevating the water scarcity issues. We aimed to assess the contribution of endogenous abscisic acid (ABA) in the protective mechanisms against water deficit, including stomatal conductance, relative water potential and the accumulation of osmoprotectants, as well as on growth parameters. To achieve that, we used a suitable model system, ABA-deficient tomato mutant, flacca and its parental line. Flacca mutant exhibited constitutively higher levels of soluble sugars (e.g., galactose, arabinose, sorbitol) and free amino acids (AAs) compared with the wild type (WT). Water deficit provoked the strong accumulation of proline in both genotypes, and total soluble sugars only in flacca. Upon re-watering, these osmolytes returned to the initial levels in both genotypes. Our results indicate that flacca compensated higher stomatal conductance with a higher constitutive level of free sugars and AAs. Additionally, we suggest that the accumulation of AAs, particularly proline and its precursors and specific branched-chain AAs in both, glucose and sucrose in flacca, and sorbitol in WT, could contribute to maintaining growth rate during water deficit and recovery in both tomato genotypes.

show abstract

Over‐accumulation of abscisic acid in transgenic tomato plants increases the risk of hydraulic failure

Cited by 29 publications

References 111 publications

SurEau: a mechanistic model of plant water relations under extreme drought

SurEau: a mechanistic model of plant water relations under extreme drought

SlTLFP8 reduces water loss to improve water‐use efficiency by modulating cell size and stomatal density via endoreduplication

Leaf Soluble Sugars and Free Amino Acids as Important Components of Abscisic Acid—Mediated Drought Response in Tomato

Contact Info

Product

Resources

About