Over a decade of experience with preimplantation genetic diagnosis: A multicenter report

Verlinsky, Yury; Cohen, Jacques; Munné, Santiago; Gianaroli, Luca; Simpson, Joe Leigh; Ferraretti, Anna Pia; Kuliev, Anver

doi:10.1016/j.fertnstert.2003.09.082

Cited by 201 publications

(107 citation statements)

References 11 publications

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“…International Working Group on PGD (2001) reported a 24% pregnancy rate through 3000 PGD cycles and data collection I-III of ESHRE PGD Consortium included an overall pregnancy rate (clinical pregnancy/OR) of 20% in PGD-AS patients. A multicenter report consisting of 3747 PGD-AS cycles provided a 23.3% pregnancy rate per embryo transfer (Verlinsky et al, 2004). As for implantation rates, Gianaroli et al (2005) studied 3-day embryos and in that prospective study, PGD-AS resulted in an implantation rate of 28% which was higher compared to that of 12% in control patients.…”

Section: Discussionmentioning

confidence: 99%

“…A decade after its introduction, PGD now offers solutions for many indications, such as chromosomal abnormalities including translocations, Mendelian disorders, and human leukocyte antigen (HLA) typing for selection of healthy and compatible embryos for transfer. In more than 6000 PGD cycles performed worldwide, approximately 1000 babies were born (Verlinsky et al, 2004). The prevalence of major congenital malformations in these babies (5-6%) was not different from that observed in the general population (ESHRE PGD Consortium Steering Committee, 2005).…”

Section: Introductionmentioning

confidence: 90%

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Successful preimplantation genetic aneuploidy screening in Turkish patients

Ercelen¹,

Turtar²,

Gültomruk³

et al. 2011

Genet. Mol. Res.

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ABSTRACT. Preimplantation genetic diagnosis is a preventive approach for identifying genetic abnormalities in early stages of reproduction. We used preimplantation genetic aneuploidy screening in 230 cycles of patients with indications of advanced maternal age, recurrent implantation failure, recurrent spontaneous abortions, or severe male factor. Biopsied blastomeres from embryos with six to eight blastomeres on day 3 were fixed and fluorescence in situ hybridization was utilized on chromosomes 13, 16, 18, 21, 22, X, and Y. Among 945 morphologically normal embryos, 314 were diagnosed as chromosomally normal. Trisomy and monosomy were observed in 36% of the cases (18% each). Embryo transfer was used in 144 cycles, resulting in 41 pregnancies. Thirty-seven healthy babies were delivered, with a take-home baby rate of 24.2% and an implantation rate of 22%. We recommend preimplantation genetic aneuploidy screening as a valuable technique to select normal chromosome embryos in order to avoid multiple pregnancies due to the multiple embryo transfers that are normally necessary to ensure pregnancy in poor prognosis in vitro fertilization patients.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 90%

Successful preimplantation genetic aneuploidy screening in Turkish patients

Ercelen¹,

Turtar²,

Gültomruk³

et al. 2011

Genet. Mol. Res.

View full text Add to dashboard Cite

show abstract

“…The first biopsy was used to perform comprehensive chromosome screening (CCS) using quantitative realtime PCR as previously described [11]. A second biopsy was used to diagnose Dyskeratosis Congenita at a reference laboratory using conventional methods of STR fragment size and Sanger sequencing as previously described [12]. After biopsies were performed all the embryos were cryopreserved to allow time for the reference laboratory to complete single gene disorder (SGD) analysis and provide a report.…”

Section: Methodsmentioning

confidence: 99%

Improved sensitivity to detect recombination using qPCR for Dyskeratosis Congenita PGD

Gueye

Jalas

Tao

et al. 2014

J Assist Reprod Genet

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“…But, whereas non-randomized PGS or PCS studies published worldwide report an increase in implantation rate and/or a decrease in miscarriage rate [11][12][13], controlled trials of PGS performed for AMA [14][15][16] and for RIF [17] have shown no benefit of PGS. Implantation and pregnancy rates were similar for women with or without PGS.…”

Section: Introductionmentioning

confidence: 99%

Are zona pellucida laser drilling and polar body biopsy safe for in vitro matured oocytes?

Hammoud

Molina-Gomès

Albert

et al. 2010

J Assist Reprod Genet

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Introduction Preconception diagnosis requires first polar body biopsy. When the hole in the zona pellucida is made with a laser beam, heat propagation could, like the biopsy itself, be deleterious. Our aim was to evaluate the effect of this technique on human in vitro matured oocyte and embryo development. Methods One hunded fifty five retrieved immature oocytes from 75 women, matured in vitro, were distributed in 3 groups: 50 oocytes in a control group, without laser drilling and first polar body biopsy, 52 oocytes in a group with only laser drilling, and 53 oocytes in a group with both laser drilling and first polar body biopsy. Safety was evaluated using four criteria: [1] oocyte lysis rate, [2] oocyte activation rate, [3] oocyte development after calcium ionophore treatment, [4] and embryo chromosome breakage incidence after Tarkowski preparation. Results No difference in the four criteria was observed between the 3 oocyte groups.Conclusions We did not find evidence of deleterious effect of laser drilling and first polar body biopsy on in vitro matured oocytes, according to our criteria.

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Over a decade of experience with preimplantation genetic diagnosis: A multicenter report

Cited by 201 publications

References 11 publications

Successful preimplantation genetic aneuploidy screening in Turkish patients

Successful preimplantation genetic aneuploidy screening in Turkish patients

Improved sensitivity to detect recombination using qPCR for Dyskeratosis Congenita PGD

Are zona pellucida laser drilling and polar body biopsy safe for in vitro matured oocytes?

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