Ovary Transplants in Drosophila Melanogaster: Studies of the Characters Singed, Fused, and Female-Sterile

Clancy, C. W.; Beadle, G. W.

doi:10.2307/1537539

Cited by 30 publications

(2 citation statements)

References 3 publications

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“…However, the correlation between Wolbachia infection and suppressor activity is not absolute, since Wolbachia -infected strain Oregon R-C, collected from the wild at the same time as the closely related strain Oregon R ( Table S1 ), was also a nonsuppressor. Oregon R-C was first reported as infected in 1994 ( Bourtzis et al 1994 ), but has been maintained in laboratories for over 50 years ( Clancy and Beadle 1937 ), and we cannot exclude the possibility that it became infected in the laboratory. Suppressor activity in the wild may therefore have been selected under the metabolic stress imposed by Wolbachia .…”

Section: Discussionmentioning

confidence: 99%

A Cytoplasmic Suppressor of a Nuclear Mutation Affecting Mitochondrial Functions inDrosophila

et al. 2012

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Phenotypes relevant to oxidative phosphorylation (OXPHOS) in eukaryotes are jointly determined by nuclear and mitochondrial DNA (mtDNA). Thus, in humans, the variable clinical presentations of mitochondrial disease patients bearing the same primary mutation, whether in nuclear or mitochondrial DNA, have been attributed to putative genetic determinants carried in the “other” genome, though their identity and the molecular mechanism(s) by which they might act remain elusive. Here we demonstrate cytoplasmic suppression of the mitochondrial disease-like phenotype of the Drosophila melanogaster nuclear mutant tko25t, which includes developmental delay, seizure sensitivity, and defective male courtship. The tko25t strain carries a mutation in a mitoribosomal protein gene, causing OXPHOS deficiency due to defective intramitochondrial protein synthesis. Phenotypic suppression was associated with increased mtDNA copy number and increased mitochondrial biogenesis, as measured by the expression levels of porin voltage dependent anion channel and Spargel (PGC1α). Ubiquitous overexpression of Spargel in tko25t flies phenocopied the suppressor, identifying it as a key mechanistic target thereof. Suppressor-strain mtDNAs differed from related nonsuppressor strain mtDNAs by several coding-region polymorphisms and by length and sequence variation in the noncoding region (NCR), in which the origin of mtDNA replication is located. Cytoplasm from four of five originally Wolbachia-infected strains showed the same suppressor effect, whereas that from neither of two uninfected strains did so, suggesting that the stress of chronic Wolbachia infection may provide evolutionary selection for improved mitochondrial fitness under metabolic stress. Our findings provide a paradigm for understanding the role of mtDNA genotype in human disease.

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Section: Discussionmentioning

confidence: 99%

A Cytoplasmic Suppressor of a Nuclear Mutation Affecting Mitochondrial Functions inDrosophila

et al. 2012

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“…Several aspects of the fused phenotype have been extensively studied: wing phenotype (Fausto-Sterling 1971, 1978, ovarian phenotype (Clancy and Beadle 1937;King et al 1957;King 1959;Bodenstein and King 1963), segmentation pattern (Martinez-Arias 1985). Wurst and Hanratty (1979), studying heat-sensitive fused alleles, found the same temperature-sensitive period, during the pupal stage, for pupal lethality and wing phenotype.…”

Section: Introductionmentioning

confidence: 99%

Genetic analysis of viable and lethalfused mutants ofDrosophila melanogaster

Busson

Limbourg-Bouchon

Mariol

et al. 1988

Roux’s Arch Dev Biol

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Fused is a segmentation gene belonging to the segment-polarity class. Mutations at thefused locus are known to display pleiotropic effects, causing zygotically determined anomalies of ovaries and of some adult cuticular structures, and maternally determined embryonic segmentation defects. In order to determine the amorphic phenotype offused and to study the genetical basis of its pleiotropy, newfused alleles (18 viable and 11 lethal) were isolated. The phenotype of these mutants and of others already known are described, taking into account zygotic and maternal effects. The main results provided by this analysis are as follows. Firstly, allfused alleles show the whole complex fused phenotype, and a good correlation is observed between the strength of the wing and segmentation defects, suggesting that a single function is involved in both processes. Secondly, all embryonic and larval lethals carry deficiencies which allow us to localizefused between the 17C4 and 17D2 bands of the X-chromosome. Thirdly, the 24 viable and 2 pupal lethals examined behave as point mutants, as shown cytologically or by Southern blot analysis. However, only one of them, the pupal lethalfu was proven to carry a nullfused allele, since it displays in germ-line clones a strong maternal phenotype and a very low zygotic rescue, similar to those of the small deficiencyDf(1)fu. The phenotype of the amorphic mutant indicates that zygotic ezpression offused is required for normal metamorphosis, while maternal expression is necessary for a normal segmentation pattern, since a complete loss offused expression during oogenesis cannot be compensated zygotically.

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Developmental physiology of the mutant female sterile(2)adipose of Drosophila melanogaster. III. Corpus allatum‐complex and ovarian transplantations

Doane

1961

J. Exp. Zool.

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Ovary Transplants in Drosophila Melanogaster: Studies of the Characters Singed, Fused, and Female-Sterile

Cited by 30 publications

References 3 publications

A Cytoplasmic Suppressor of a Nuclear Mutation Affecting Mitochondrial Functions inDrosophila

A Cytoplasmic Suppressor of a Nuclear Mutation Affecting Mitochondrial Functions inDrosophila

Genetic analysis of viable and lethalfused mutants ofDrosophila melanogaster

Developmental physiology of the mutant female sterile(2)adipose of Drosophila melanogaster. III. Corpus allatum‐complex and ovarian transplantations

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