Ovary and ovulation: DNA fragmentation of oocytes in aged mice

Fujino, Yuko; Ozaki, Keisuke; Yamamasu, Seiichi; Ito, Fumihiro; Matsuoka, I.; Hayashi, Eiji; Nakamura, Hideyoshi; Ogita, Sachio; Sato, Eisuke F.; Inoue, Masayasu

doi:10.1093/oxfordjournals.humrep.a019421

Cited by 153 publications

(129 citation statements)

References 9 publications

Supporting

Mentioning

116

Contrasting

Unclassified

Order By: Relevance

“…young mice, older counterparts show poor response to superovulation [5][6][7], increased aneuploidy rates [6][7][8] and increased FSH levels [9]. Recent data also demonstrated that oocytes from aged females contained higher levels of DNA damage [10]. These oocytes show decreased fertilization rates [11] due to delayed pronuclear formation [7] and abnormal calcium oscillations [12].…”

Section: Electronic Supplementary Materialsmentioning

confidence: 99%

The association between the oocyte pool and aneuploidy: a comparative study of the reproductive potential of young and aged mice

Cheng

Hou

et al. 2013

J Assist Reprod Genet

View full text Add to dashboard Cite

Purpose The present study examined the effect of aging on female reproductive potential. Methods Six-week-old and 9-month-old CD1 mice were referred to as the 'young' and 'aged' groups, respetively. Oocytes were collected after superovulation, and their viability were compared using parthenogenetic activation. The aneuploidy of the oocytes (MII) was assessed using chromosome spread, and the whole ovarian follicle number was counted using an unbiased stereological method. Serum hormone levels were measured using the radio-immunity method, and the expression of the Cohesin subunit genes in the oocytes (GV) were assessed using RT-PCR. Results The mean number of recovered (25.8 vs. 16.2; P<0.05) and live oocytes (24.0 vs. 11.73; P <0.01) per head in the young-mice group (6-week-old) was significantly higher than that of the aged group (9-month-old). The aneuploidy rate of the ovulated oocytes in the aged group was significantly higher than that of the young group (36.8 % vs. 10 %; P<0.01), and the rate of blastocyst formation in the young group (85.23 %) was significantly higher than that of the aged group (81.2 %; P <0.05). The number of primordial follicles (the oocyte pool) per ovary in the aged group was significantly decreased compared with the young group (330±33.51 vs. 2079.6±420.70; P<0.01), and the level of AMH in the aged group was significantly higher than that of the young group (4.66±0.11 ng/ml vs. 4.07±0.18 ng/ml; P<0.01). Conclusions We propose that maternal aging significantly reduces the oocyte pool, superovulation efficiency and developmental potential and increases the oocyte aneuploidy rate.

show abstract

Section: Electronic Supplementary Materialsmentioning

confidence: 99%

The association between the oocyte pool and aneuploidy: a comparative study of the reproductive potential of young and aged mice

Cheng

Hou

et al. 2013

J Assist Reprod Genet

View full text Add to dashboard Cite

show abstract

“…It is now clear that this spontaneous decay process in vivo as well as in vitro involves cellular fragmentation and ultimately death. [1][2][3] Cytoplasmic fragmentation has also been observed in oocytes and embryos after several in vitro paradigms. 4,5 Despite the fact that spontaneous fragmentation of the unfertilized oocyte has been documented almost three decades ago, very little is known about the cellular and molecular mechanisms governing this process.…”

Section: Introductionmentioning

confidence: 99%

Molecular requirements for doxorubicin-mediated death in murine oocytes

Jurisicova

et al. 2006

Cell Death Differ

View full text Add to dashboard Cite

We previously published evidence that oocytes exposed to doxorubicin (DXR), a widely used chemotherapeutic agent, rapidly undergo morphological and biochemical changes via discrete effector signaling pathways consistent with the occurrence of apoptosis. In this report, we elucidated the molecular requirements for actions of this drug in oocytes. Our results indicate that within 1 h of exposure DXR causes rapid DNA damage, and commits the oocyte to cytoplasmic fragmentation by the fourth hour, followed by delayed oocyte activation and execution of cytoplasmic fragmentation. Inhibitors that interfere with oocyte activation consistently rescue cytoplasmic fragmentation, but fail to suppress DNA damage. There was evidence of depletion of Bax, Caspase-2, MA-3 and Bcl-x transcripts, suggesting that modulations by DXR caused recruitment of these maternal transcripts into the translation process. Furthermore, sphingolipids such as sphingosine-1-phosphate and ceramide modulate DXR actions by, respectively, altering its intracellular trafficking, or by sustaining the drug's contact with DNA.

show abstract

“…The oocytes, like spermatozoa, may contain fragmented or damaged DNA independent of the number of chromosomes, as has been demonstrated in the rat (5). The aim of our study was to demonstrate the presence of damaged DNA in unfertilized oocytes after ICSI treatment, considering that apoptotic events in these cells could occur before treatment and thus lead to fertilization failure.…”

mentioning

confidence: 76%

“…Moreover, DNA fragmentation in oocytes associated with apoptotic evidence might be one of the reasons for poor oocyte quality and lower fertility in aged mice (5). Of greater concern would be discerning whether early apoptotic events are present in human oocytes treated with advanced techniques of assisted reproduction, in particular with intracytoplasmic sperm injection (ICSI), in which a single sperm is injected into an oocyte.…”

mentioning

confidence: 99%

Apoptosis in human unfertilized oocytes after intracytoplasmic sperm injection

Bosco

Ruvolo

Morici

et al. 2005

Fertility and Sterility

View full text Add to dashboard Cite

Objective: To investigate the presence of programmed cell death in unfertilized oocytes after intracytoplasmic sperm injection (ICSI), assuming that previous apoptotic events could be correlated with the fertilization failure. Design: Comparison of the rate of DNA fragmentation in human oocytes at different stages of maturation soon after pick-up (control) and in unfertilized oocytes after ICSI treatment. Result(s):The DNA fragmentation, by TUNEL assay, appeared in all the immature control oocytes, but only 37% of mature oocytes showed DNA fragmentation. This DNA fragmentation was observed in 88.8% of the oocytes unfertilized after ICSI; furthermore, DNA fragmentation appeared as well in the sperm injected into the cytoplasm. Conclusion(s):The study has shown DNA fragmentation in human oocytes unfertilized after ICSI. The evidence is confirmed as well in control oocytes, free from in vitro culture or manipulation stress. Caspase-3 immunoassay suggests the presence of apoptosis. The high percentage of oocytes demonstrating DNA fragmentation in the unfertilized oocytes could be correlated with fertilization failure. (Fertil Steril 2005;84:1417-23.

show abstract

Ovary and ovulation: DNA fragmentation of oocytes in aged mice

Cited by 153 publications

References 9 publications

The association between the oocyte pool and aneuploidy: a comparative study of the reproductive potential of young and aged mice

The association between the oocyte pool and aneuploidy: a comparative study of the reproductive potential of young and aged mice

Molecular requirements for doxorubicin-mediated death in murine oocytes

Apoptosis in human unfertilized oocytes after intracytoplasmic sperm injection

Contact Info

Product

Resources

About