Ovariectomy increases the formation of prostanoids and modulates their role in acetylcholine-induced relaxation and nitric oxide release in the rat aorta

Martorell, Aina; Sagredo, Ana; Aras-López, Rosa; Balfagón, Gloria; Ferrer, Mercedes

doi:10.1093/cvr/cvp214

Cited by 14 publications

(21 citation statements)

References 41 publications

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“…The results showed that after short periods of gonadal function loss the production of TXA 2 was already increased up to similar levels of those observed in vessels from 5 months post-orchidectomized rats. This increased release of TXA 2 over time, could be involved in the maintenance of NO release during a prolonged period of time after gonadectomy, as observed in aorta from male ( unpublished results ) and female rats [21].…”

Section: Discussionmentioning

confidence: 60%

“…In aorta from rats, we reported that the effect of ovariectomy on NO release depended on the time lapsed since the loss of the gonads [20]. This implies that different compensatory mechanisms are likely to be at work during prolonged periods of time after gonadectomy preventing vascular failure, as already suggested [21]. In view of these data, our first objective in the present study was to determine whether there were differences in the release of NO and TXA 2 in aorta and mesenteric arteries of rats subjected to short (6 weeks) and long (5 months) periods post-orchidectomy.…”

Section: Introductionmentioning

confidence: 82%

“…For analgesia, rats were treated with 0.30 mg/Kg SC meloxicam (Metacam from Boehringer-Ingelheim) immediately after surgery and with 50 mg/Kg ibuprofen for 4 days. Systolic blood pressure was indirectly measured in animals 2–3 days before sacrifice by the tail-cuff method [21] using a Leica Digital Pressure Meter LE5000 (Barcelona, Spain). Extraction of blood samples (by cardiac puncture) for testosterone levels determination and body weight measurement were done the day of the experiment before the animals were sacrificed by CO 2 inhalation.…”

Section: Methodsmentioning

confidence: 99%

See 2 more Smart Citations

Time-Dependent Effect of Orchidectomy on Vascular Nitric Oxide and Thromboxane A2 Release. Functional Implications to Control Cell Proliferation through Activation of the Epidermal Growth Factor Receptor

et al. 2014

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This study analyzes whether the release of nitric oxide (NO) and thromboxane A2 (TXA2) depends on the time lapsed since gonadal function is lost, and their correlation with the proliferation of vascular smooth muscle cells (VSMC) mediated by the epidermal growth factor receptor (EGFR). For this purpose, aortic and mesenteric artery segments from control and 6-weeks or 5-months orchidectomized rats were used to measure NO and TXA2 release. The results showed that the basal and acetylcholine (ACh)-induced NO release were decreased 6 weeks post-orchidectomy both in aorta and mesenteric artery, but were recovered 5 months thereafter up to levels similar to those found in arteries from control rats. The basal and ACh-induced TXA2 release increased in aorta and mesenteric artery 6 weeks post-orchidectomy, and was maintained at high levels 5 months thereafter. Since we previously observed that orchidectomy, which decreased testosterone level, enlarged the muscular layer of mesenteric arteries, the effect of testosterone on VSMC proliferation was analyzed. The results showed that treatment of cultured VSMC with testosterone downregulated mitogenic signaling pathways initiated by the ligand-dependent activation of the EGFR. In contrast, the EGFR pathways were constitutively active in mesenteric arteries of long-term orchidectomized rats. Thus, the exposure of mesenteric arteries from control rats to epidermal growth factor (EGF) induced the activation of EGFR signaling pathways. However, the addition of EGF to arteries from orchidectomized rats failed to induce a further activation of these pathways. In conclusion, this study shows that the release of NO depends on the time lapsed since the gonadal function is lost, while the release of TXA2 is already increased after short periods post-orchidectomy. The alterations in these signaling molecules could contribute to the constitutive activation of the EGFR and its downstream signaling pathways after long period post-orchidectomy enhancing the proliferation of the vascular muscular layer.

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Section: Discussionmentioning

confidence: 60%

Section: Introductionmentioning

confidence: 82%

Section: Methodsmentioning

confidence: 99%

See 1 more Smart Citation

Time-Dependent Effect of Orchidectomy on Vascular Nitric Oxide and Thromboxane A2 Release. Functional Implications to Control Cell Proliferation through Activation of the Epidermal Growth Factor Receptor

et al. 2014

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“…Arachidonic acid is metabolized by COX to a variety of vasoactive substances, including prostacyclin, prostaglandin E 2 , or thromboxane A 2 . 15 Prostaglandin E 2 only induced a small relaxation response in very low concentration (Ͻ3 nmol/L) in mouse aorta (Fig 1). This relaxation response was nearly completely abolished after 2 days of cold storage.…”

Section: Resultsmentioning

confidence: 89%

Functional, morphologic, and molecular characterization of cold storage injury

Ebner

Poitz

Augstein

et al. 2012

Journal of Vascular Surgery

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“…Systolic blood pressure was indirectly measured in awake animals by the tail-cuff method [17, 25] before and after the treatment using a Letica Digital Pressure Meter LE5000 (Barcelona, Spain).…”

Section: Methodsmentioning

confidence: 99%

Docosahexaenoic Acid Supplemented Diet Influences the Orchidectomy-Induced Vascular Dysfunction in Rat Mesenteric Arteries

et al. 2017

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Over the past few decades, the cardiovascular benefits of a high dietary intake of long-chain polyunsaturated fatty acids (PUFAs), like docosahexaenoic acid (DHA), have been extensively studied. However, many of the molecular mechanisms and effects exerted by PUFAs have yet to be well explained. The lack of sex hormones alters vascular tone, and we have described that a DHA-supplemented diet to orchidectomized rats improve vascular function of the aorta. Based on these data and since the mesenteric artery importantly controls the systemic vascular resistance, the objective of this study was to analyze the effect of a DHA-supplemented diet on the mesenteric vascular function from orchidectomized rats. For this purpose mesenteric artery segments obtained from control, orchidectomized or orchidectomized plus DHA-supplemented diet were utilized to analyze: (1) the release of prostanoids, (2) formation of NO and ROS, (3) the vasodilator response to acetylcholine (ACh), as well as the involvement of prostanoids and NO in this response, and (4) the vasoconstrictor response to electrical field stimulation (EFS), analyzing also the effect of exogenous noradrenaline (NA), and the NO donor, sodium nitroprusside (SNP). The results demonstrate beneficial effects of DHA on the vascular function in orchidectomized rats, which include a decrease in the prostanoids release and superoxide formation that were previously augmented by orchidectomy. Additionally, there was an increase in endothelial NO formation and the response to ACh, in which NO involvement and the participation of vasodilator prostanoids were increased. DHA also reversed the decrease in EFS-induced response caused by orchidectomy. All of these findings suggest beneficial effects of DHA on vascular function by reversing the neurogenic response and the endothelial dysfunction caused by orchidectomy.

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Ovariectomy increases the formation of prostanoids and modulates their role in acetylcholine-induced relaxation and nitric oxide release in the rat aorta

Abstract: Despite the prevalence of vasoconstrictor prostanoids derived from COX-2 in aortas from ovariectomized rats, the ACh-induced relaxation is maintained, probably as consequence of the positive regulation that prostanoids exert on eNOS activity.

Cited by 14 publications

References 41 publications

Time-Dependent Effect of Orchidectomy on Vascular Nitric Oxide and Thromboxane A2 Release. Functional Implications to Control Cell Proliferation through Activation of the Epidermal Growth Factor Receptor

Time-Dependent Effect of Orchidectomy on Vascular Nitric Oxide and Thromboxane A2 Release. Functional Implications to Control Cell Proliferation through Activation of the Epidermal Growth Factor Receptor

Functional, morphologic, and molecular characterization of cold storage injury

Docosahexaenoic Acid Supplemented Diet Influences the Orchidectomy-Induced Vascular Dysfunction in Rat Mesenteric Arteries

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