Ovariectomy decreases plasma triglyceride levels in analbuminaemic rats by lowering hepatic triglyceride secretion

Joles, Jaap A.; Bijleveld, Caspaar; Tol, Arie van; Geelen, M.J.H.; Koomans, Hein A.

doi:10.1016/0021-9150(95)05557-d

Cited by 17 publications

(12 citation statements)

References 36 publications

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“…Similar results have been reported by the same authors and others in analbuminemic and obese Zucker rats [40, 41, 42]. The question is, how could we explain the beneficial effect of estrogens observed in our experiment.…”

Section: Discussionsupporting

confidence: 80%

Hyperlipidemic Nephropathy Induced by Adriamycin in Ovariectomized Rats: Role of Free Radicals and Effect of 17-Beta-Estradiol Administration

Montilla

Túnez

Muñoz

et al. 2000

Nephron

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The present study in rats evaluates the effects of the ovariectomy (OVX) with and without the simultaneous administration of 17β-estradiol (17βE₂) on oxidative stress and hyperlipidemic nephropathy induced by a single high dose of adriamycin (AD). OVX enhances oxidative stress and worsens nephropathy induced by AD. These changes are prevented by simultaneous administration of 17βE₂. OVX alone induced oxidative stress and hyperlipidemia without biochemical evidence of renal dysfunction. Our results revealed that ovarian hormones, especially estrogens, have a protective effect against oxidative stress and nephropathy induced by AD. Since the reactive oxygen species plays a role in the pathogenesis of renal lesion, it is important to emphasize that estrogens and their hydroxylated compounds function as biological antioxidants.

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Section: Discussionsupporting

confidence: 80%

Hyperlipidemic Nephropathy Induced by Adriamycin in Ovariectomized Rats: Role of Free Radicals and Effect of 17-Beta-Estradiol Administration

Montilla

Túnez

Muñoz

et al. 2000

Nephron

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“…8), the first step of fatty acids b-oxidation and DLAT (Fig. 8), which is subunit 2 of the pyruvate dehydrogenase complex (the findings that ovariectomy represses and estrogens stimulate the synthesis of TAG by the liver [78] agree with these results); 3) acetate through the ACSS1 (Fig. 8), known to be induced under ketogenic conditions [79,80].…”

Section: Enzymes Of Acetyl-coa Production and Cholesterol Synthesissupporting

confidence: 83%

Changes in the Proteome of Functional and Regressing Corpus Luteum During Pregnancy and Lactation in the Rat1

González-Fernández¹,

Martı́nez-Galisteo²,

Gaytán³

et al. 2008

Biology of Reproduction

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The corpus luteum (CL) is an exquisitely regulated transitory endocrine gland necessary for the onset and maintenance of pregnancy in mammals. Most of the data on the mechanisms of CL differentiation at the molecular level come from genomic studies, but direct protein data are scarce. Here we have undertaken a differential expression proteomic approach to identify, in an unbiased way, those proteins whose levels change significantly in the rat CL as it evolves from functionality during pregnancy to regression after parturition. Moreover, we have compared the regressing CL with the newly formed functional CL that coexist during lactation under the same endocrine environment. We have defined a "proteomic signature" of CL functionality, which is constituted by a set of 24 proteins with a few differences between pregnancy and lactation. Most of these markers are new and are involved in microtubule assembly, retinoic acid transport, and Raf kinase signaling cascade; 10 are enzymes that define a ketogenic metabolic landscape, demonstrating, for the first time, the prevalence of de novo cholesterol synthesis in luteal cells. The "proteomic signature of regression," on the other hand, is composed of nine proteins, one of which is 20alpha-hydroxysteroid dehydrogenase and two, ferritin and gamma-actin, are new. The discovery of unpredictable new actors in the differentiation process of CL reported here will contribute to new hypotheses that explain the complex female reproductive function at the protein level. It will also open new doors to research on each identified protein by relating them to cellular differentiation.

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“…This is because intracellular cholesterol in female NAR was similar to, and microsomal cholesterol was actually lower than, that found in the male NAR. The greater VLDL and IDL cholesterol levels in female NAR compared with male and OVX NAR are most likely related to the previously reported higher secretion rate and lower clearance of the triglyceride-rich lipoproteins in female than in male and OVX NAR (5,15). The lower clearance of VLDL and chylomicrons in the female than male NAR was due primarily to the lower activities of lipoprotein lipase and hepatic lipase that were restored by OVX in the female NAR (5,15).…”

Section: Discussionmentioning

confidence: 72%

“…The greater VLDL and IDL cholesterol levels in female NAR compared with male and OVX NAR are most likely related to the previously reported higher secretion rate and lower clearance of the triglyceride-rich lipoproteins in female than in male and OVX NAR (5,15). The lower clearance of VLDL and chylomicrons in the female than male NAR was due primarily to the lower activities of lipoprotein lipase and hepatic lipase that were restored by OVX in the female NAR (5,15). Together, greater production and lower clearance of the apoB-containing lipoproteins in the female than in the male NAR play an important part in gender differences in plasma cholesterol and triglyceride levels in these animals.…”

Section: Discussionmentioning

confidence: 81%

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Effects of gender on hepatic HMG-CoA reductase, cholesterol 7α-hydroxylase, and LDL receptor in hereditary analbuminemia

Shin¹,

Vaziri²,

Willekes³

et al. 2005

American Journal of Physiology-Endocrinology and Metabolism

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Hypoalbuminemia is accompanied by hypercholesterolemia in both nephrotic syndrome and hereditary analbuminemia. Hypercholesterolemia is more severe in the female than in the male Nagase analbuminemic rats (NAR). The sex difference in plasma cholesterol diminishes after ovariectomy (OVX) and reappears after estrogen replacement in the NAR. The molecular mechanism responsible for the sex difference in severity of hypercholesterolemia in NAR is not known and was investigated here. To this end, hepatic hydroxylmethylglutaryl (HMG)-CoA reductase, cholesterol 7␣-hydroxylase, and LDL receptor were determined in male, female, and OVX female NAR and Sprague-Dawley (SD) rats. Plasma cholesterol, triglycerides, and hepatic HMG-CoA reductase activities were greater in both female and male NAR than in SD rats. This was coupled with upregulation of cholesterol 7␣-hydroxylase in both male and female NAR compared with SD controls. LDL receptor in male NAR was similar to that in male SD rats but was significantly reduced in female NAR. OVX partially, but significantly, reduced plasma cholesterol and triglyceride levels in female NAR. This was coupled with a significant rise in hepatic cholesterol 7␣-hydroxylase and a modest increase in hepatic LDL receptor. In contrast, OVX resulted in a mild elevation of plasma cholesterol and no significant changes in total hepatic HMG-CoA reductase, cholesterol 7␣-hydroxylase, or LDL receptor in female SD rats. Thus the greater severity of hypercholesterolemia in the female NAR appears to be due, in part, to a combination of the constrained compensatory upregulation of cholesterol 7␣-hydroxylase and LDL receptor deficiency.hyperlipidemia; hypercholesterolemia; nephrotic syndrome; proteinuria; low-density lipoprotein; hydroxymethylglutaryl-CoA reductase; bile acids HEAVY GLOMERULAR PROTEINURIA (nephrotic syndrome) results in hypoalbuminemia, diminished plasma oncotic pressure, and a marked rise in plasma total cholesterol concentration (14). Hypercholesterolemia, albeit less severe, is also present the Nagase rats (2,3,11,13,14) and humans (1) with hereditary analbuminemia in whom proteinuria is absent. These observations suggest that hypoalbuminemia, per se, can raise plasma cholesterol concentration. Hypercholesterolemia is more severe in the female Nagase analbuminemic rat (NAR) than in the male NAR (11,14). The difference in plasma cholesterol concentration between the male and female NAR diminishes after ovariectomy (OVX) and reappears with estrogen replacement (7-10). Similarly, estrogen administration aggravates hypercholesterolemia in rats with adriamycin-induced nephrotic syndrome, wherein hypoalbuminemia is due to proteinuria (4). In contrast, orchiectomy does not change plasma cholesterol in the male NAR (8). These observations suggest that the effect of hypoalbuminemia on plasma cholesterol concentration is amplified by an ovarian factor, most likely estrogen.In an earlier study (6), we found a significant increase in extrahepatic cholesterol synthesis in NAR. In a subsequent study...

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Ovariectomy decreases plasma triglyceride levels in analbuminaemic rats by lowering hepatic triglyceride secretion

Cited by 17 publications

References 36 publications

Hyperlipidemic Nephropathy Induced by Adriamycin in Ovariectomized Rats: Role of Free Radicals and Effect of 17-Beta-Estradiol Administration

Hyperlipidemic Nephropathy Induced by Adriamycin in Ovariectomized Rats: Role of Free Radicals and Effect of 17-Beta-Estradiol Administration

Changes in the Proteome of Functional and Regressing Corpus Luteum During Pregnancy and Lactation in the Rat1

Effects of gender on hepatic HMG-CoA reductase, cholesterol 7α-hydroxylase, and LDL receptor in hereditary analbuminemia

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