Ovarian transplantation for fertility preservation in a sheep model: can follicle loss be prevented by antiapoptotic sphingosine-1-phosphate administration?

Hancke, Katharina; Walker, Elisabeth W.; Strauch, Oliver; Göbel, Heike; Hanjalic-Beck, Aida; Denschlag, Dominik

doi:10.3109/09513590903159524

Cited by 22 publications

(13 citation statements)

References 27 publications

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“…Its protective effects during ovarian tissue cryopreservation and transplantation are more controversial. When used during auto-transplantation of fresh ovine tissue, no beneficial effect was observed [ 26 ]. The same effect was described when SIP was added to the freezing media of whole ovine ovaries [ 40 ] or to the culture media of ovarian tissue after slow freezing or vitrification [ 41 ].…”

Section: Discussionmentioning

confidence: 99%

“…Anti-apoptotic drugs have also been evaluated during tissue transplantation. In an auto-transplantation model of fresh sheep ovarian fragments into the abdominal wall, S1P did not show a beneficial effect [ 26 ]. However, the use of the same drug in xenografts of fresh human ovarian cortex in immunodeficient mice improved angiogenesis and decreased follicular apoptosis [ 27 ].…”

Section: Introductionmentioning

confidence: 99%

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Supplementation of transport and freezing media with anti-apoptotic drugs improves ovarian cortex survival

et al. 2016

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BackgroundOvarian tissue preservation is proposed to patients at risk of premature ovarian failure, but this procedure still needs to be optimized. To limit injury during ovarian tissue cryopreservation, anti-apoptotic drugs were added to the transport and freezing media of ovarian cortex tissue.MethodsSheep ovaries were transported, prepared and frozen in solutions containing vehicle or anti-apoptotic drugs (Z-VAD-FMK, a pan-caspase inhibitor, or sphingosine-1-phosphate (S1P), a bioactive lipid). After the tissue was thawed, the ovarian cortex was cultured for 2 or 6 days. Follicular quantification and morphological and proliferation analyses were performed on histological sections.ResultsAfter 2 days of culture, S1P improved the quality of primordial follicles; higher densities of morphologically normal and proliferative primordial follicles were found. Z-VAD-FMK displayed similar effects by preserving global primordial follicular density, but this effect was evident after 6 days of culture. This drug also improved cell proliferation after 2 and 6 days of culture.ConclusionsOur results showed that the addition of S1P or Z-VAD-FMK to the transport and freezing media prior to ovarian tissue cryopreservation improves primordial follicular quality and therefore improves global tissue survival. This should ultimately lead to improved fertility restoration after auto-transplantation.Electronic supplementary materialThe online version of this article (doi:10.1186/s13048-016-0216-0) contains supplementary material, which is available to authorized users.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Supplementation of transport and freezing media with anti-apoptotic drugs improves ovarian cortex survival

et al. 2016

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“…In the field of fertility preservation, sphingosine-1-phosphate (S1P) and its agonists are largely studied for their combined anti-apoptotic and pro-angiogenic properties. While the ability of S1P to protect follicles from chemotherapy and radiationinduced damages has been well demonstrated [25][26][27][28][29], the protective effects of S1P in cryopreserved tissue grafting studies remain controversial [30][31][32][33]. Another alternative, a permeable synthetic peptide pan-caspase inhibitor, benzyloxycarbonyl-Val-Ala-Asp-fluoromethyl ketone (Z-VAD-FMK), has only been studied once in a xenograft model of cryopreserved ovarian tissue after producing encouraging results as a therapeutic option [34].…”

Section: Introductionmentioning

confidence: 97%

In vitro evaluation of the anti-apoptotic drug Z-VAD-FMK on human ovarian granulosa cell lines for further use in ovarian tissue transplantation

Fransolet

Henry

Labied

et al. 2015

J Assist Reprod Genet

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“…Many reports have suggested that damage of cryopreserved ovarian grafts might be induced by initial ischemia and/or ischemic perfusion injury rather than from vitrification-associated processes [30][31][32][33][34][35][36][37][38]. Several attempts have been made to prevent the follicular loss of cryopreserved ovarian tissues after transplantation [14,15,[38][39][40][41][42][43]. In the dog, the effect of desialylated erythropoietin on the follicular survival of vitrified ovaries after xenotransplantation has been reported [14,15].…”

Section: Discussionmentioning

confidence: 99%

Incidence of Apoptotic Cells After Vitrification in Canine Ovarian Tissues

Hariya¹,

Suzuki

2016

Journal of Mammalian Ova Research

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Although vitrification and follicular survival subsequent to xenotransplantation of canine ovarian tissues have been reported, the degree of cryoinjury after the vitrification has not yet been fully examined. Since cryoinjury after vitrification of ovaries has been reported in bovine and humans, this study evaluated canine ovarian tissues after vitrification by immunohistochemistry and TUNEL assay. Ovaries were collected from immature bitches. The ovarian tissue cubes were pretreated with 1 M DMSO. Subsequently, DAP 213 solution (2 M DMSO, 1 M acetamide, 3 M propylene glycol) was added. The cryotubes containing the ovarian tissues were placed on ice for 5 min, then plunged directly into liquid nitrogen. After warming with 0.25 M sucrose, cryopreserved ovarian tissues were fixed and subsequently stained with hematoxylin-eosin, proliferation cell nuclear antigen (PCNA) antibody, or active caspase-3 (AC-3) antibody, and evaluated by the TUNEL assay. There were no differences in the numbers of primordial, primary, secondary and antral follicles/mm 2 between the fresh and vitrified-warmed ovarian tissues. Percentages of PCNA and AC-3 antibody positive cells were similar regardless of the developmental stage of the follicles and experimental group. A few TUNEL positive cells were detected in both experimental groups. These results suggest that vitrification with DAP213 does not induce cryoinjury in ovarian tissues from immature bitches.

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Ovarian transplantation for fertility preservation in a sheep model: can follicle loss be prevented by antiapoptotic sphingosine-1-phosphate administration?

Abstract: Apoptosis seems to play a role in follicular loss during ovarian transplantation; however, local application of S1P does not prevent primordial/primary follicles from hypoxia-induced cell death in cortical grafts in our study.

Cited by 22 publications

References 27 publications

Supplementation of transport and freezing media with anti-apoptotic drugs improves ovarian cortex survival

Supplementation of transport and freezing media with anti-apoptotic drugs improves ovarian cortex survival

In vitro evaluation of the anti-apoptotic drug Z-VAD-FMK on human ovarian granulosa cell lines for further use in ovarian tissue transplantation

Incidence of Apoptotic Cells After Vitrification in Canine Ovarian Tissues

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