Ovarian Tissue Cryopreservation for Fertility Preservation

Jensen, Annette Klüver; Kristensen, Stine Gry; Andersen, Claus Yding

doi:10.1016/b978-0-12-801591-9.00007-2

Cited by 2 publications

(2 citation statements)

References 62 publications

(42 reference statements)

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“…Issues related to human fertility preservation have been increasingly addressed worldwide, especially when cancer treatments are being discussed 1 . Ovarian tissue cryopreservation followed by transplantation has become a promising strategy to restore fertility 2 , 3 . This approach not only enables the preservation and restoration of female fertility in adult or prepubertal patients, it also facilitates the restoration of ovarian endocrine function 1 , 2 .…”

Section: Introductionmentioning

confidence: 99%

Metabolic activity in cryopreserved and grafted ovarian tissue using high-resolution respirometry

Rodrigues

Goulart

Silva

et al. 2021

Sci Rep

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Cryopreservation of ovarian tissue followed by transplantation represents a strategy to restore ovarian function and fertility. Stress from cryopreservation-thawing processes can lead to alterations and/or damage to mitochondrial structure and functionality. High resolution respirometry and histological analysis were used to evaluate the effect of cryopreservation and transplantation on ovarian tissue. Four different conditions were performed: Fresh non-transplanted tissue, Fresh transplanted tissue, Cryopreserved non-transplanted tissue and Cryopreserved transplanted tissue. All groups were able to respond to the substrates-uncoupler-inhibitor protocol. We found a dramatic decrease in general oxygen consumption in hemi-ovaries submitted to cryopreservation and/or transplantation. The effect of cryopreservation on mitochondrial metabolism was less intense than effect of transplantation, since the transplantation affected all of the mitochondrial states. A total of 2644 follicles were analyzed. Of these, 2198 were classified as morphologically normal. The percentage of morphologically normal follicles was significantly lower in the Cryopreserved transplanted group when compared to the Cryopreserved non-transplanted group and the Fresh transplanted group (p-value < 0.05). Despite decreased follicular viability and mitochondrial activity, the cryopreservation followed by transplantation of ovarian tissue proved feasible for attempts to restore ovarian function.

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Section: Introductionmentioning

confidence: 99%

Metabolic activity in cryopreserved and grafted ovarian tissue using high-resolution respirometry

Rodrigues

Goulart

Silva

et al. 2021

Sci Rep

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“…The leading aim of ovarian tissue cryopreservation is to maintain the viability of oocytes from preantral follicles, a promising approach that has been studied in many species [1][2][3]. In addition to showing normal morphology, cryopreserved tissue samples must be able to resume functionality, which has been successfully assessed by auto or xenotransplantation [4,5]. To date, restoration of ovarian function has been demonstrated in mice [6], sheep [7,8], cows [9], goats [10], and rabbits [11], and more than 130 live births have been reported in humans [12] after transplantation of cryopreserved tissue.…”

Section: Introductionmentioning

confidence: 99%

Function of Cryopreserved Cat Ovarian Tissue after Autotransplantation

Vilela

Leonel

Gonçalves

et al. 2019

Animals

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The aim of this study was to assess a slow-freezing protocol of cat ovarian tissue cryopreservation using autotransplantation. Four adult queens were ovariohysterectomized and the ovaries were fragmented and cryopreserved. After one week, the grafts were thawed and autografted to the subcutaneous tissue of the dorsal neck of each queen, then randomly removed after 7, 14, 28, 49, and 63 days after transplantation. Percentages of morphologically normal primordial and growing follicles (MNFs) were 88% and 97%, respectively, in fresh tissue samples (fresh controls), and 74% and 100%, respectively, immediately after thawing (cryo D0). No MNFs were found after 49 days of transplantation. In both fresh control and cryo D0 fragments, granulosa cells were frequently in proliferation. Two morphologically normal antral follicles were detected in one queen on Day 28 post-transplantation. Connective tissue fibers increased, suggesting replacement of active ovarian cortex by fibrous tissue. Tissue vascularization was observed at 7 days after grafting, and wide blood vessels were clearly visible on Days 49 and 63. In conclusion, although follicular survival was low after cryopreservation and grafting of cat ovarian tissue, follicles were able to develop up to the antral stage, which is an encouraging outcome.

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Ovarian Tissue Cryopreservation for Fertility Preservation

Cited by 2 publications

References 62 publications

Metabolic activity in cryopreserved and grafted ovarian tissue using high-resolution respirometry

Metabolic activity in cryopreserved and grafted ovarian tissue using high-resolution respirometry

Function of Cryopreserved Cat Ovarian Tissue after Autotransplantation

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