Ovarian tissue cryopreservation by stepped vitrification and monitored by X-ray computed tomography

Corral, Ariadna; Clavero, Macarena; Gallardo, Miguel; Balcerzyk, Marcin; Amorim, Christiani Andrade; Parrado-Gallego, Ángel; Dolmans, Marie-Madeleine; Paulini, Fernanda; Morris, John; Risco, Ramón

doi:10.1016/j.cryobiol.2018.03.001

Cited by 19 publications

(15 citation statements)

References 28 publications

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“…As an alternative, liquidus tracking systems have been developed, aiming to reduce tissue/follicle damage from these concentrations. Stepped vitrification may be performed and the cell response to CPA toxicity may be attenuated, since the sample is only subjected to high concentrations of CPA when low temperatures are experienced in the local environment, hence lowering cell metabolism and activity [34,35]. Some examples of cryopreservation solutions and equilibration curves applied before storage are shown in Table 1.…”

Section: Determining the Cryopreservation Protocolmentioning

confidence: 99%

Cryopreservation of Preantral Follicles

Leonel¹,

Lucci²,

Amorim³

2018

Cryopreservation Biotechnology in Biomedical and Biological Sciences

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In mammals, the total number of female germ cells is already established by the time of birth, meaning that no mitosis will take place in oogonias thereafter. Their cryostorage, therefore, depends on ovarian tissue manipulation. As an alternative to mature oocyte cryopreservation, the maintenance of inactive preantral follicles is a remarkable option because (i) their availability in the ovary is greater; (ii) as inactive and small structures, they show less sensitivity to cryoinjury and the toxic effects of cryoprotectants; and (iii) they are present in the gonads at all ages, allowing their retrieval from prepubertal individuals or even immediately postmortem. Nevertheless, some difficulties remain regarding their in vitro activation and development to the ovulatory stage. For this reason, the best option for their total development is transplantation back to the donor or between species, promoting follicle activation and development. This technique has proved its efficiency and led to several live births in both animals and humans. Since each species has its own particularities in terms of ovarian tissue composition, a number of protocols have been documented, which may be used for either isolated or in situ preantral follicles.

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Section: Determining the Cryopreservation Protocolmentioning

confidence: 99%

Cryopreservation of Preantral Follicles

Leonel¹,

Lucci²,

Amorim³

2018

Cryopreservation Biotechnology in Biomedical and Biological Sciences

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“…The difference between their strategy and equilibrium vitrification is that the decline in CPA toxicity with temperature and reduction in the system melting point do not need to follow the same curve [57]. Corral et al [57] tested their new procedure to vitrify bovine ovarian tissue by increasing the DMSO concentration in increments while simultaneously decreasing the temperature in increments, using a device specifically designed for this purpose. Their promising findings revealed follicles with histological integrity after warming similar to fresh controls [57].…”

Section: Future Trends In Otcmentioning

confidence: 99%

“…Corral et al [57] tested their new procedure to vitrify bovine ovarian tissue by increasing the DMSO concentration in increments while simultaneously decreasing the temperature in increments, using a device specifically designed for this purpose. Their promising findings revealed follicles with histological integrity after warming similar to fresh controls [57]. …”

Section: Future Trends In Otcmentioning

confidence: 99%

“…However, vitrification is based on three crucial steps: addition of high CPA concentrations and achievement of high cooling and warming rates to decrease CPA toxicity. To avoid the toxic CPA effects, Corral et al [57] developed a procedure called “stepped vitrification,” which consists of gradually decreasing the temperature while CPA is increased. The difference between their strategy and equilibrium vitrification is that the decline in CPA toxicity with temperature and reduction in the system melting point do not need to follow the same curve [57].…”

Section: Future Trends In Otcmentioning

confidence: 99%

“…To avoid the toxic CPA effects, Corral et al [57] developed a procedure called “stepped vitrification,” which consists of gradually decreasing the temperature while CPA is increased. The difference between their strategy and equilibrium vitrification is that the decline in CPA toxicity with temperature and reduction in the system melting point do not need to follow the same curve [57]. Corral et al [57] tested their new procedure to vitrify bovine ovarian tissue by increasing the DMSO concentration in increments while simultaneously decreasing the temperature in increments, using a device specifically designed for this purpose.…”

Section: Future Trends In Otcmentioning

confidence: 99%

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Cryopreservation of Human Ovarian Tissue: A Review

Leonel

Lucci

Amorim

2019

Transfus Med Hemother

117

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Background: Cryopreservation of human ovarian tissue has been increasingly applied worldwide to safeguard fertility in cancer patients, notably in young girls and women who cannot delay the onset of their treatment. Moreover, it has been proposed to patients with benign pathologies with a risk of premature ovarian insufficiency. So far, more than 130 live births have been reported after transplantation of cryopreserved ovarian tissue, and almost all patients recovered their ovarian function after tissue reimplantation. Summary: This review aims to summarize the recent results described in the literature regarding human ovarian tissue cryopreservation in terms of methods and main results obtained so far. To cryopreserve human ovarian tissue, most studies describe a slow freezing/rapid thawing protocol, which is usually an adaptation of a protocol developed for sheep ovarian tissue. Since freezing has been shown to have a deleterious effect on ovarian stroma and granulosa cells, various research groups have been vitrifying ovarian tissue. Despite promising results, only 2 babies have been born after transplantation of vitrified/warmed ovarian tissue. Optimization of both cryopreservation strategies as well as thawing/warming protocols is therefore necessary to improve the survival of follicles in cryopreserved ovarian tissue. Key Messages: Human ovarian tissue cryopreservation has been successfully applied worldwide to preserve fertility in patients with malignant or nonmalignant pathologies that have a detrimental effect on fertility. Human ovarian tissue cryopreservation could also be applied as an alternative to postpone pregnancy or menopause in healthy women. Slow freezing and vitrification procedures have been applied to cryopreserve human ovarian tissue, but both alternatives require optimization.

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