Ovarian superstimulatory response and embryo development using a new recombinant glycoprotein with eCG-like activity in mice

Crispo, Martina; Meikle, María Noel; Schlapp, Geraldine; Menchaca, A.

doi:10.1016/j.theriogenology.2021.01.012

Cited by 14 publications

(11 citation statements)

References 10 publications

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“…In sheep, a similar result has been reported by Gibson et al (2004) where eCG induced an earlier surge of the pre‐ovulatory LH. It has been reported that ovarian stimulation can be induced either using FSH or eCG in several species including the cow (Small et al, 2009), sheep (Cognie, 1999), pigs (Manjarin et al, 2009), goats (Montes‐Quiroz et al, 2019), mouse (Crispo et al, 2021) and rats (Popova et al, 2002), and it not only increased the number of follicles but also increased the transition of small to medium follicles (Sarwar et al, 2020). Thus, it is plausible to speculate that the FSH‐like activity of eCG might have influenced the stimulation of follicular growth and increased the secretion of E 2 by the pre‐ovulatory follicles as observed in the present experiment.…”

Section: Discussionmentioning

confidence: 99%

Effect of eCG dose on ovarian haemodynamics, hormonal profiles and prolificacy rate when oestrus was induced during low‐breeding season in Beetal goats

Sharif

Hassan

Arshad

et al. 2022

Reprod Domestic Animals

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The objectives of the experiment were to determine the effect of two doses of equine chorionic gonadotropin (eCG) in a standard synchronization protocol based on a short‐term progesterone (P4) priming on ovarian structures and haemodynamics, concentrations of steroid hormones and prolificacy rate when oestrus was induced during low‐breeding season (LBS) in Beetal dairy goats. We hypothesized that inclusion of eCG in a short‐term P4 priming‐based synchronization protocol would increase the blood perfusion to ovarian structures leading to enhance oestrous and ovulatory responses and prolificacy rate in goats. Forty‐two multiparous acyclic goats were blocked by body condition and, within block, assigned randomly to receive saline as control (CON), low eCG (L‐eCG; 300 IU) or high eCG (H‐eCG; 600 IU) dose. Initially, a controlled internal drug release (CIDR) device was placed in the anterior vagina on d −8, followed by removal of CIDR on d −3, concurrent with the administration of PGF2α and eCG according to their respective treatments. Goats were monitored for oestrous response. B‐mode and Doppler ultrasonography was performed with 12‐h interval, starting from day −3 until natural breeding (day 0), and then on days 5, 10, 15 and 20 post‐breeding to monitor follicular and luteal dynamics and blood flow, respectively. Blood was sampled at 0, 12, 24, 36 and 60 h after CIDR removal to quantify plasma concentrations of estradiol‐17β (E2), whereas plasma concentrations of P4 were assayed at days 5, 10, 15 and 20 after breeding. Pregnancy and prolificacy rates were determined at day 30 and 150 after breeding, respectively. Data were analysed with mixed‐effects models, and orthogonal contrasts were used to evaluate the effect of treatment [Con vs. (½ L‐eCG + ½ H‐eCG)] and dose of eCG (L‐eCG vs. H‐eCG). Data are presented in sequence as CON, L‐eCG, H‐eCG (LSM ± SEM). The oestrous intensity score (152.9 vs. 182.7 vs. 186.5 ± 15.1; p = .02) was greater in eCG‐treated goats as compared to CON. Administration of eCG reduced the intervals to standing oestrus (66.2 vs. 41.8 vs. 48.9 h ± 5.5; p = .05), breeding (70.2 vs. 44.4 vs. 45.4 h ± 4.5; p = .03) and ovulation (84.5 vs. 61.2 vs. 63.4 h ± 6.2; p = .05) compared with CON goats. The mean growth rate of pre‐ovulatory follicle was greater (1.11 vs. 1.49 vs. 1.45 mm ± 0.08; p = .01) in eCG‐treated goats resulting in an increased diameter of pre‐ovulatory follicle (6.27 vs. 7.20 vs. 7.31 mm ± 0.07; p < .01) and corpora lutea (6.75 vs. 8.26 vs. 8.07 mm ± 0.42; p = .04) than CON. The mean follicular blood flow did not differ among treatments; however, the mean luteal blood flow was greater in L‐eCG‐treated goats (0.81 vs. 1.61 vs. 1.07 cm2 ± 0.12; p = .001). The mean concentrations of E2 (4.03 vs. 5.21 vs. 4.78 pg/ml ± 0.42; p = .04) and P4 (4.85 vs. 6.39 vs. 6.22 ng/ml ± 0.34; p = .04) were greater in eCG‐treated goats. The twinning rate did not differ between treatments; nevertheless, prolificacy rate was greater (p = .04) in L‐eCG‐treated goats. Collectively, our data sugges...

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Section: Discussionmentioning

confidence: 99%

Effect of eCG dose on ovarian haemodynamics, hormonal profiles and prolificacy rate when oestrus was induced during low‐breeding season in Beetal goats

Sharif

Hassan

Arshad

et al. 2022

Reprod Domestic Animals

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“… 24 C57BL/6J females were subjected to oestrus synchronization and superovulation. 25 Eight females were mated with eight fertile males each time, and the pronucleus was injected with approximately 10 pL mRNA. Approximately forty injected embryos were transferred into the fallopian tube of each pseudopregnant mouse.…”

Section: Methodsmentioning

confidence: 99%

Vasorin deficiency leads to cardiac hypertrophy by targeting MYL7 in young mice

Guo

Liang

et al. 2021

J Cellular Molecular Medi

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Vasorin (VASN), also known as SLIT-like 2 (slitl2), is a 673-amino acid transmembrane glycoprotein localized on the cell surface 1 that is cleaved by a disintegrin and metalloproteinase. VASN is considered a potential biomarker and therapeutic target for cancer. 2,3 Notably, VASN is highly conserved in many organs from embryonic development to adulthood. 4,5 There is limited literature on VASN function.In embryonic development, VASN is primarily expressed in the heart and lungs. 1 VASN is expressed in vascular smooth muscle cells 1 and

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“…High-yield ovulation, however, can result in production of up to 100 oocytes/female, thereby reducing the number of females needed for superovulation 123,125 . In the future, recombinant eCG may help to replace the animal-derived drug, once commercially available 126 . The mating of prepubescent females after superovulation is controversially discussed as an animal welfare issue.…”

Section: Production Of Oocytes and Embryosmentioning

confidence: 99%

3R measures in facilities for the production of genetically modified rodents

2022

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Sociocultural changes in the human-animal relationship have led to increasing demands for animal welfare in biomedical research. The 3R concept is the basis for bringing this demand into practice: Replace animal experiments with alternatives where possible, Reduce the number of animals used to a scientifically justified minimum and Refine the procedure to minimize animal harm. The generation of gene-modified sentient animals such as mice and rats involves many steps that include various forms of manipulation. So far, no coherent analysis of the application of the 3Rs to gene manipulation has been performed. Here we provide guidelines from the Committee on Genetics and Breeding of Laboratory Animals of the German Society for Laboratory Animal Science to implement the 3Rs in every step during the generation of genetically modified animals. We provide recommendations for applying the 3Rs as well as success/intervention parameters for each step of the process, from experiment planning to choice of technology, harm-benefit analysis, husbandry conditions, management of genetically modified lines and actual procedures. We also discuss future challenges for animal welfare in the context of developing technologies. Taken together, we expect that our comprehensive analysis and our recommendations for the appropriate implementation of the 3Rs to technologies for genetic modifications of rodents will benefit scientists from a wide range of disciplines and will help to improve the welfare of a large number of laboratory animals worldwide.

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Ovarian superstimulatory response and embryo development using a new recombinant glycoprotein with eCG-like activity in mice

Cited by 14 publications

References 10 publications

Effect of eCG dose on ovarian haemodynamics, hormonal profiles and prolificacy rate when oestrus was induced during low‐breeding season in Beetal goats

Effect of eCG dose on ovarian haemodynamics, hormonal profiles and prolificacy rate when oestrus was induced during low‐breeding season in Beetal goats

Vasorin deficiency leads to cardiac hypertrophy by targeting MYL7 in young mice

3R measures in facilities for the production of genetically modified rodents

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