Ovarian Gonadotropin-Releasing Hormone (GnRH) Receptors: Characterization, Distribution, and Induction by GnRH*

Pieper, David R.; Richards, John O.; Marshall, John C.

doi:10.1210/endo-108-4-1148

Cited by 131 publications

(51 citation statements)

References 34 publications

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“…The first evidence for the presence of ovarian GNRHR, through ligand-specific binding sites, was observed in rat granulosa and luteal cells (Reeves et al 1980, Pieper et al 1981, Olofsson et al 1995. Subsequently, the presence of GNRHR mRNA was also identified in human granulosa luteal cells (Kang et al 2000) and in both follicles and CL of bovine ovary (Ramakrishnappa et al 2001).…”

Section: Discussionmentioning

confidence: 99%

Expression of type I GNRH receptor and in vivo and in vitro GNRH-I effects in corpora lutea of pseudopregnant rabbits

Zerani¹,

Parillo²,

Brecchia³

et al. 2010

Journal of Endocrinology

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The expression of type I GNRH receptor (GNRHR-I) and the direct role of GNRH-I on corpora lutea (CL) function were studied in the pseudopregnant rabbit model. Immunohistochemistry evidenced GNRHR-I and GNRH-I in luteal cells at early (day 4 pseudopregnancy)-, mid (day 9)-, and late (day 13)-luteal stages. Real-time RT-PCR and western blotting revealed GNRHR-I mRNA and protein at the three luteal stages. Buserelin in vivo treatment at days 9 and 13 decreased plasma progesterone levels for 48 and 24 h respectively. In in vitro cultured CL, buserelin reduced progesterone secretion, increased prostaglandin F 2a (PGF 2a ) secretion and cyclooxygenase-2 (COX-2) and nitric oxide synthase (NOS) activities at days 9 and 13, and decreased PGE 2 at day 13. Co-incubation with antagonists for GNRH-I (antide), inositol 1,4,5-trisphosphate (IP 3 , 2-amino-ethoxydiphenylborate), and diacylglycerol (DAG, 1-hexadecyl-2-acetyl glycerol) or inhibitors for phospholipase C (PLC, compound 48/80), and protein kinase C (PKC, staurosporine) counteracted the buserelin effects. Buserelin co-incubated with COX inhibitor (acetylsalicylic acid) increased progesterone and decreased PGF 2a and NOS activity at days 9 and 13, whereas co-incubation with NOS inhibitor (N-nitro-L-arginine methyl ester) increased progesterone at the same luteal stages. These results suggest that GNRHR-I is constitutively expressed in rabbit CL independently of luteal stage, whereas GNRH-I down-regulates directly CL progesterone production via PGF 2a at mid-and late-luteal stages of pseudopregnancy, utilizing its cognate type I receptor with a post-receptorial mechanism that involves PLC, IP 3 , DAG, PKC, COX-2, and NOS.

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Section: Discussionmentioning

confidence: 99%

Expression of type I GNRH receptor and in vivo and in vitro GNRH-I effects in corpora lutea of pseudopregnant rabbits

Zerani¹,

Parillo²,

Brecchia³

et al. 2010

Journal of Endocrinology

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“…This fact shows the participation of the peripheral nervous system in ovarian function (Condon & Black 1976, Coleman et al 1979, Harwood et al 1980, Adashi & Hsueh 1981, Aguado et al 1982, Aguado & Ojeda 1984a,b, Norjavaara et al 1984, Sosa et al 2000. Furthermore, some neurotransmitters that stimulate ovarian progesterone release such as noradrenaline and vasoactive intestinal peptide (Ojeda & Aguado 1985, Dees et al 1986, Ojeda & Lara 1989, Dissen et al 1993, Gerendai et al 1995, Kalekzyc et al1995, or peptides with inhibitory effects such as gonadotrophinreleasing hormone (Hsueh & Erickson 1979, Huesh & Jones 1981, Pieper et al 1981, Sheela Rani et al 1983) and gamma aminobutyric acid (Erdö et al 1985, Häppölä et al 1987 have been found in the ovary. Some of them are also present in the SON and the coeliac ganglion (Jan & Jan 1981, 1982, 1983, Sejnowski 1982, Dalsgaard et al 1983, Karhula et al 1988, Lascar et al 1996.…”

Section: Introductionmentioning

confidence: 99%

Coeliac ganglion adrenergic activity modifies ovarian progesterone during pregnancy: its inter-relationship with LH

Casais

Sosa²,

Rastrilla³

et al. 2001

Journal of Endocrinology

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Most of the fibres that constitute the superior ovarian nerve (SON) originate at the neuronal bodies of the coeliac ganglion and innervate rat ovarian stroma cells. The purpose of this work was to study the part played by innervation on ovarian release of progesterone on day 15 and at the end of pregnancy in an integrated in vitro system known as the coeliac ganglion-SON-ovary system.We also investigated, in the same system, whether there is some kind of inter-relationship between the effect of adrenergic agents and LH on progesterone release on day 15 of pregnancy.The coeliac ganglion and the ovary were incubated in separate compartments, linked by the SON. The ovary was immersed in 2 ml buffer solution (ovarian compartment) and the coeliac ganglion was immersed in 2 ml of a different buffer solution (ganglion compartment). Under these conditions, the accumulation of progesterone in the ovarian compartment medium was used as an endpoint. Conditions were standardised on day 15 of pregnancy, when the decrease in the release of ovarian progesterone caused by non-specific stimulation on the ganglion with KCl (56 mM) demonstrated the functional integrity of the system. Neural influence was evaluated by the addition of adrenergic agents at a concentration of 10 6 M to the coeliac ganglion. On day 15 of pregnancy, noradrenaline and propranolol increased progesterone release while phentolamine diminished it. The existence of ganglionic tone was assessed by analysing progesterone basal levels at different stages of pregnancy. The highest secretion of progesterone was found to take place on day 15, diminishing as pregnancy advanced. In addition, adrenergic neural participation was studied during the physiological luteolysis occurring at the end of pregnancy. Major findings were that noradrenaline increased ovarian accumulation of progesterone on day 19 and decreased it on day 20, while propranolol and phentolamine diminished progesterone release on both days. In additional studies, some neuroendocrine aspects were investigated at a peripheral level. The addition of LH only to the ovarian compartment did not affect progesterone secretion. However, when LH in the ovarian compartment was accompanied by noradrenaline, propranolol or phentolamine in the ganglion compartment, the release of progesterone decreased.It can be concluded that modifications of the neural state of the coeliac ganglion affect ovarian progesterone secretion and the physiology of pregnancy via the SON. The results may confirm that the coeliac ganglion-SONovary system provides a direct link between the autonomic nervous system and physiological events during pregnancy.

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“…Since follicular tissue has been reported to contain higher concentrations of LHRH receptors than luteal tissue (Pieper, Richards & Marshall, 1981) …”

Section: Resultsmentioning

confidence: 99%

Changes in pituitary and ovarian LHRH receptors after active immunization of female rats against LH or LHRH

Popkin¹,

Fraser²

1985

Reproduction

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Ovarian Gonadotropin-Releasing Hormone (GnRH) Receptors: Characterization, Distribution, and Induction by GnRH*

Cited by 131 publications

References 34 publications

Expression of type I GNRH receptor and in vivo and in vitro GNRH-I effects in corpora lutea of pseudopregnant rabbits

Expression of type I GNRH receptor and in vivo and in vitro GNRH-I effects in corpora lutea of pseudopregnant rabbits

Coeliac ganglion adrenergic activity modifies ovarian progesterone during pregnancy: its inter-relationship with LH

Changes in pituitary and ovarian LHRH receptors after active immunization of female rats against LH or LHRH

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