Ovarian antiproliferative activity directed isolation of triterpenoids from fruits of Eucalyptus camaldulensis Dehnh

Topçu, Gülaçtı; Yapar, Gönül; Türkmen, Zeynep; Gören, Ahmet; Öksüz, Sevïl; Schilling, Jennifer K.; Kingston, David G. I.

doi:10.1016/j.phytol.2011.05.002

Cited by 25 publications

(13 citation statements)

References 24 publications

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“…Preliminary results with fraction F2-4-25 ( Figure 5(a) ), rich in compound 1 in glucose uptake in C2C12 cells, suggest that this molecule is involved in E. tereticornis hypoglycemia effects ( Figure 5(b) ). The structure shown in Figure 5(c) corresponds to 3 β -hydroxy-urs-11-en-28,13 β -olide, a molecule previously described in E. tereticornis with antimicrobial activity [ 15 ] and in E. camaldulensis, showing an antiproliferative effect on ovarian cancer lines [ 35 ]. Despite the large number of pharmacological actions that are attributed to Eucalyptus spp., among them antidiabetic, this is the first time we determine one of the potential compounds responsible for such activity.…”

Section: Discussionmentioning

confidence: 99%

Antihyperglycemic Activity ofEucalyptus tereticornisin Insulin-Resistant Cells and a Nutritional Model of Diabetic Mice

Guillen

Granados

Rivas

et al. 2015

Advances in Pharmacological Sciences

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Eucalyptus tereticornis is a plant used in traditional medicine to control diabetes, but this effect has not been proved scientifically. Here, we demonstrated through in vitro assays that E. tereticornis extracts increase glucose uptake and inhibit their production in insulin-resistant C2C12 and HepG2 cells, respectively. Furthermore, in a nutritional model using diabetic mice, the administration of ethyl acetate extract of E. tereticornis reduced fasting glycaemia, improved tolerance to glucose, and reduced resistance to insulin. Likewise, this extract had anti-inflammatory effects in adipose tissue when compared to control diabetic mice. Via bioguided assays and sequential purification of the crude extract, a triterpenoid-rich fraction from ethyl acetate extracts was shown to be responsible for the biological activity. Similarly, we identified the main compound responsible for the antihyperglycemic activity in this extract. This study shows that triterpenes found in E. tereticornis extracts act as hypoglycemic/antidiabetic compounds and contribute to the understanding of their use in traditional medicine.

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Section: Discussionmentioning

confidence: 99%

Antihyperglycemic Activity ofEucalyptus tereticornisin Insulin-Resistant Cells and a Nutritional Model of Diabetic Mice

Guillen

Granados

Rivas

et al. 2015

Advances in Pharmacological Sciences

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“…In Anatolia, Eucalyptus plants have also been used in folk medicine for antiseptic, antimicrobial, astringent, and deodorant purposes, as well as anti-fever, stimulant, antispasmodic, and haemostatic agents (Ashour, 2008;Mulyaningsih et al, 2010;Topçu et al, 2011). Eucalyptus also contains a wide variety of phenolic compounds with antioxidant, antiproliferative, anti-inflammatory, anti-hyperglycemic, and anti-thrombotic properties (Gilles et al, 2010;Mota et al, 2012;Santos et al, 2012).…”

Section: Introductionmentioning

confidence: 99%

Investigation of phytochemicals and antioxidant capacity of selected Eucalyptus species using conventional extraction

et al. 2015

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Eucalyptus species have found their place in traditional medicine and pharmacological research and they have also been shown to possess a large number of phenolic compounds and antioxidants. The present study sought to implement conventional extraction to yield maximal total phenolic content (TPC), total flavonoid content (TFC), proanthocyanidins, antioxidants, and saponins from E. robusta using different solvents. The most suitable extraction solvent was further employed for extracting phytochemicals from E. saligna, E. microcorys, and E. globulus to select the Eucalyptus species with the greatest bioactive compound content. The results emphasised the efficiency of water in extracting TPC ((150.60 ± 2.47) mg of gallic acid equivalents per g), TFC ((38.83 ± 0.23) mg of rutin equivalents per g), proanthocyanidins ((5.14 ± 0.77) mg of catechin equivalents per g), and antioxidants ABTS ((525.67 ± 1.99) mg of trolox equivalents (TE) per g), DPPH ((378.61 ± 4.72) mg of TE per g); CUPRAC ((607.43 ± 6.69) mg of TE per g) from E. robusta. Moreover, the aqueous extract of E. robusta had the highest TPC, TFC and antioxidant values among the other Eucalyptus species tested. These findings highlighted the efficiency of conventional extraction in extracting natural bioactive compounds from Eucalyptus species for pharmaceutical and nutraceutical applications.

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“…fruits have been found to inhibit proliferation of the A2780 human ovarian cancer cell line. [19] Triterpenoids from the roots of Astilbe myriantha Diels have found to be anti-fungal agents. [20]…”

Section: Discussionmentioning

confidence: 99%

Modulation of genotoxicity of oxidative mutagens by glycyrrhizic acid from Glycyrrhiza glabra L.

et al. 2012

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Background:The chemopreventive effects of certain phytoconstituents can be exploited for their use as functional foods, dietary supplements and even as drugs. The natural compounds, acting as anti-genotoxic and free radical scavenging compounds, may serve as potent chemopreventive agents. These can inhibit DNA modulatory activities of mutagens and help preventing pathological processes.Objectives:Present study on Glycyrrhiza glabra L., a promising medicinal plant, widely used in traditional medicine, focused on the bioassay-guided fractionation of its extracts for the isolation of certain phytochemicals with anti-genotoxic potential against oxidative mutagens.Materials and Methods:The methanol extract of Glycyrrhiza glabra rhizomes was subjected to column chromatography, and isolated fraction was evaluated for its anti-genotoxic and antioxidant potential using SOS chromotest, Comet assay, and DPPH radical scavenging assay.Results:GLG fraction, which was characterized as Glycyrrhizic acid, inhibited the genotoxicity of oxidative mutagens viz., H2O2 and 4NQOquite efficiently. In SOS chromotest, using E.coli PQ37 tester strain, it inhibited induction factor induced by H2O2 and 4NQO by 75.54% and 71.69% at the concentration of 121.46 μM,respectively. In Comet assay, it reduced the tail moment induced by H2O2 and 4NQO by 70.21% and 69.04%, respectively, at the same concentration in human blood lymphocytes. The isolated fraction also exhibited DPPH free radical scavenging activity and was able to scavenge 85.95% radicals at a concentration of 120 μM.Conclusion:Glycyrrhizic acid is a potential modulator of genotoxins as well as efficient scavenger of free radicals.

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Ovarian antiproliferative activity directed isolation of triterpenoids from fruits of Eucalyptus camaldulensis Dehnh

Cited by 25 publications

References 24 publications

Antihyperglycemic Activity ofEucalyptus tereticornisin Insulin-Resistant Cells and a Nutritional Model of Diabetic Mice

Antihyperglycemic Activity ofEucalyptus tereticornisin Insulin-Resistant Cells and a Nutritional Model of Diabetic Mice

Investigation of phytochemicals and antioxidant capacity of selected Eucalyptus species using conventional extraction

Modulation of genotoxicity of oxidative mutagens by glycyrrhizic acid from Glycyrrhiza glabra L.

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