Ovarian and Circulating Inhibin Levels in Immature Female Rats Treated with Gonadotropin and after Castration*

Lee, Victor W. K.; McMaster, J D; Quigg, Helen; Leversha, Lorraine

doi:10.1210/endo-111-6-1849

Cited by 58 publications

(22 citation statements)

References 5 publications

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“…FSH administration also stimulated follicular growth and maturation, and positive staining for inhibin sub units was demonstrated in the granulosa cells of preantral and antral follicles. The result is in accord with the pre vious results that FSH increases inhibin niRNA levels in the hypophvsectomized rat ovary [ 16] and that FSH stim ulates inhibin production by the granulosa cells [5,6,[17][18][19], The intensity of immunostaining was higher in antral granulosa cells than in mural granulosa cells. It is sug gested that antral and mural granulosa cells might re spond differently to FSH.…”

Section: Discussionsupporting

confidence: 90%

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Effects of Estrogen, Pituitary Gonadotropins and Prolactin on Immunohistochemical Localization of Inhibin Subunits in the Ovary of Hypophysectomized Female Rats

Kobayashi¹,

Yamoto²,

Minami³

et al. 1992

Horm Res

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Effects of estrogen, pituitary gonadotropins and prolactin on immunohistochemical localization of α- and β_A-subunits in the ovaries of hypophysectomized female rats were investigated. Hypophysectomy resulted in disappearance of immunoreactive inhibin subunits in the ovary. Administration of DES, FSH and LH to hypophysectomized rats provoked growth of follicles, and resulted in positive immunostaining for inhibin subunits in the granulosa cells. In contrast to follicle-stimulating hormone (FSH) and luteinizing hormone (LH), prolactin administration failed to demonstrate positive staining for inhibin subunits in the ovary. The present in vivo results suggest that several hormones which are known to stimulate granulosa cell growth and maturation, such as estrogen, FSH and LH, enhance inhibin subunit production by the overy. The morphologic aspect of inhibin subunit production by the ovary in response to several hormones has been demonstrated in the present study.

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Section: Discussionsupporting

confidence: 90%

“…Experiments have shown that ovarian cells, such as granulosa cells, are able to produce inhibin [ 1 -3], and that the removal of the ovary markedly decreases measurable inhibin [4,5]. Using a specific radioimmunoassay which detects the N-terminal portion of the inhibin a-subunit.…”

Section: Introductionmentioning

confidence: 99%

Effects of Estrogen, Pituitary Gonadotropins and Prolactin on Immunohistochemical Localization of Inhibin Subunits in the Ovary of Hypophysectomized Female Rats

Kobayashi¹,

Yamoto²,

Minami³

et al. 1992

Horm Res

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“…More study is required to determine how the changes in follicular numbers and health (including the smaller follicles not sampled in this study) contribute to the level of inhibin secretion by the ovary. However, comparison of the temporal patterns of follicular fluid inhibin activity and changes in the follicular population suggest that there are other factors influential in determining the secretion rate of inhibin, such as hormonal input (Channing et al, 1981 a ;Lee et al, 1982) and state of follicular maturation (Channing et al, 1981a). The changes in inhibin activity in follicular fluid could be partly a reflection of the steroidogenic capacity of the follicle.…”

Section: Discussionmentioning

confidence: 99%

“…Rat ovarian venous effluent (DePaolo, Shander, Wise, Barraclough & Channing 1979a) and medium conditioned by primary culture of rat follicular granulosa cells (Erickson & Hsueh, 1978) have been shown to contain inhibin activity. Inhibin activity has also been detected in the cytosol of ovarian homogenates of immature rats and in the serum of PMSGtreated immature female rats (Lee, McMaster, Quigg & Leversha, 1982). However, the presence of inhibin activity in rat follicular fluid has not been reported.…”

Section: Introductionmentioning

confidence: 99%

Changes in inhibin activity, and progesterone, oestrogen and androstenedione concentrations, in rat follicular fluid throughout the oestrous cycle

Fujii¹,

Hoover²,

Channing³

1983

Reproduction

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Follicular fluid was aspirated from all visible surface follicles of rats at selected times of the oestrous cycle. Fluids from a pair of rat ovaries were pooled and assayed for inhibin activity by the rat anterior pituitary cell culture assay. Serum LH, FSH and progesterone as well as follicular fluid progesterone, total oestrogens and androstenedione were also measured. Follicular fluid inhibin activity was relatively constant throughout the oestrous cycle (30.7 +/- 3.4% inhibition of FSH per 0.1 microliter follicular fluid) except for a well defined surge at pro-oestrus (09:00-16:00 h, peak at 14:00 h = 84.0 +/- 7.2% inhibition of FSH per 0.1 microliter follicular fluid). The follicular fluid was not treated with charcoal before assay because a pilot experiment showed that such treatment did not alter the inhibin activity of follicular fluid. Steroids in follicular fluid were generally lowest on the afternoon of oestrus and the morning of dioestrus I and generally elevated during pro-oestrus.

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“…The amount of activin in bovine follicular fluid seems quite low ( 5%) compared with that of inhibin. Administration of charcoal-treated follicular fluid reduces serum FSH concentrations in female rats [34], and bilateral ovariectomy of eCG-primed immature female rats is followed by an increase in circulating FSH levels [35]. Furthermore, it is likely that activin is associated with a binding protein, FSP, which would neutralize its biological activity (see below).…”

Section: Fig 4 the In Vitro Effect Of Activin On (A) Basal And (B) mentioning

confidence: 99%

An Update on the Roles of Inhibin, Activin, and Follistatin as Local Regulators of Folliculogenesis1

Findlay¹

1993

Biology of Reproduction

400

211

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The local actions of the inhibin-related peptides inhibin, activin, and follistatin in folliculogenesis, luteinization and atresia are reviewed. On the basis of in vitro data, it is concluded that (a) inhibin has a paracrine action positively regulating LH-induced androgen production by theca cells in addition to a peripheral role regulating FSH secretion; (b) activin has a negative paracrine action on LH-induced androgen production by theca cells; (c) activin, either alone or with FSH, has an autocrine action on granulosa cells, promoting differentiation during the preantral and early antral stages of folliculogenesis and preventing premature luteinization in the later stages of antral follicle development, leading overall to promotion and maintenance of the folliculogenic state of the follicle; and (d) follistatin modulates granulosa cell function in favor of luteinization or atresia, and its mechanism of action involves neutralizing the actions of activin by its binding properties and by a direct action of follistatin on progesterone metabolism by granulosa cells. A hypothesis for a role of activin in the acquisition of responsiveness of granulosa cells of preantral follicles to FSH is proposed.

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Ovarian and Circulating Inhibin Levels in Immature Female Rats Treated with Gonadotropin and after Castration*

Cited by 58 publications

References 5 publications

Effects of Estrogen, Pituitary Gonadotropins and Prolactin on Immunohistochemical Localization of Inhibin Subunits in the Ovary of Hypophysectomized Female Rats

Effects of Estrogen, Pituitary Gonadotropins and Prolactin on Immunohistochemical Localization of Inhibin Subunits in the Ovary of Hypophysectomized Female Rats

Changes in inhibin activity, and progesterone, oestrogen and androstenedione concentrations, in rat follicular fluid throughout the oestrous cycle

An Update on the Roles of Inhibin, Activin, and Follistatin as Local Regulators of Folliculogenesis1

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