Ovalbumin‐specific IgE/IgG4 ratio might improve the prediction of cooked and uncooked egg tolerance development in egg‐allergic children

Vazquez‐Ortiz, Marta; Pascal, Mariona; Jiménez‐Feijoo, Rosa; Lozano, J.J.; Giner, Ma Teresa; Alsina, Laia; Martín-Mateos, María Anunciación; Plaza, Ana María

doi:10.1111/cea.12273

Cited by 48 publications

(69 citation statements)

References 36 publications

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“…Typically, such blocking properties are distinctive hallmarks of the IgG4 antibody subclass [5]. According to the number of studies sIgG4 level as well as the ratio sIgE/sIgG4 is associated with likelihood of allergic symptoms reporting [6, 7] and can improve the prediction of tolerance to some allergens [8]. Therefore, sIgG4 detection together with sIgE measurement gives more inclusive information for in vitro analysis interpretation.…”

Section: Introductionmentioning

confidence: 99%

Development of a microarray-based method for allergen-specific IgE and IgG4 detection

et al. 2017

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BackgroundsIgE and sIgG4 detection is necessary for more accurate and effective type I hypersensitivity diagnostics and the estimation of disease development. Typically, the analyses of these antibodies are performed separately with the help of various specialized systems. The aim of this study was to develop a microarray-based method for the simultaneous quantitative detection of sIgE and sIgG4 to the most common allergens in a single sample.MethodsA quantitative method for the simultaneous detection of sIgE and sIgG4 was developed based on the technology of hydrogel microchips previously designed at Engelhardt Institute of Molecular Biology, Russian Academy of Sciences (EIMB RAS). The microarray contained gel pads with immobilized allergens and gel pads that allow for the obtaining of sIgE and sIgG4 internal calibration curves for each allergen during the assay. The possibility of the simultaneous detection of sIgE and sIgG4 was developed using the corresponding Cy5 and Cy3 fluorescent dyes.ResultsThe multiplex immunoassay method using hydrogel microarrays developed in this study allowed the quantitative detection of sIgE and sIgG4 to 31 allergens from different groups in a single assay. A comparison of the microarray with the existing plate-based analogues (i.e., ALLERG-O-LIQ and sIgG4 ELISA) was performed by analysing 152 blood serum samples and by evaluating Pearson correlation coefficients, ROC analysis, and Passing-Bablok linear regression results.ConclusionThe implementation of this method in allergy diagnostics will provide the possibility of simultaneously performing primary patient screening and obtaining additional information concerning the severity of the allergies and the choice of an appropriate therapy.

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Section: Introductionmentioning

confidence: 99%

Development of a microarray-based method for allergen-specific IgE and IgG4 detection

et al. 2017

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“…Mechanistic studies of oral IT to food allergens, although limited to date, show that plasma markers such as IgE and IgG4 immunoglobulins, skin test markers, component testing, and basophil activation tests are only weakly predictive of long-term clinical success (10)(11)(12)(13)(14)(15)(16). T cells are critical upstream regulators of allergic sensitization that are required to help B cells to synthesize IgE/IgG4 immunoglobulins, which then can activate or inhibit basophils and mast cells (10)(11)(12)(13)(14)(15)(16)(17). Moreover, successful IT is associated with the development of regulatory T cells (Tregs) that are thought to dampen allergic reactivity to offending allergens (7).…”

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confidence: 99%

Successful immunotherapy induces previously unidentified allergen-specific CD4+ T-cell subsets

Ryan

Hovde

Glanville

et al. 2016

Proc. Natl. Acad. Sci. U.S.A.

126

124

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Allergen immunotherapy can desensitize even subjects with potentially lethal allergies, but the changes induced in T cells that underpin successful immunotherapy remain poorly understood. In a cohort of peanut-allergic participants, we used allergen-specific T-cell sorting and single-cell gene expression to trace the transcriptional "roadmap" of individual CD4+ T cells throughout immunotherapy. We found that successful immunotherapy induces allergen-specific CD4+ T cells to expand and shift toward an "anergic" Th2 T-cell phenotype largely absent in both pretreatment participants and healthy controls. These findings show that sustained success, even after immunotherapy is withdrawn, is associated with the induction, expansion, and maintenance of immunotherapy-specific memory and naive T-cell phenotypes as early as 3 mo into immunotherapy. These results suggest an approach for immune monitoring participants undergoing immunotherapy to predict the success of future treatment and could have implications for immunotherapy targets in other diseases like cancer, autoimmune disease, and transplantation.is a process in which small amounts of allergen are given over time to the allergic individual until they can safely tolerate high amounts of allergen with no signs of clinical symptoms (1-9). In the regimen of oral IT for peanut-allergic patients (identified by an allergic reaction during a standardized blinded food challenge to peanut), small amounts of peanut flour protein are ingested and escalated to a servings-worth of peanut protein (4 g of peanut protein) over a period of 2-3 y (6-7). Most patients require continuous frequent (e.g., daily) exposure to such therapy for beneficial clinical outcomes. Mechanistic studies of oral IT to food allergens, although limited to date, show that plasma markers such as IgE and IgG4 immunoglobulins, skin test markers, component testing, and basophil activation tests are only weakly predictive of long-term clinical success (10-16). T cells are critical upstream regulators of allergic sensitization that are required to help B cells to synthesize IgE/IgG4 immunoglobulins, which then can activate or inhibit basophils and mast cells (10-17). Moreover, successful IT is associated with the development of regulatory T cells (Tregs) that are thought to dampen allergic reactivity to offending allergens (7). We therefore focused on finding T-cell markers of immune tolerance that could be detected early in the peripheral blood during IT. CD4+ T cells can be relatively long-lived (compared with plasma proteins and basophils) and changes detected early in populations of T cells could perhaps predict longer-lasting successful IT. For example, in one of the first studies in peanut allergen IT to withdraw therapy for more than 10 wk, we previously showed that despite negative skin tests to peanut, and high IgG4/low specific IgE levels to peanut, and decreased basophil reactivity to the allergen, some patients who withdrew from therapy for 3-6 mo were still reactive upon rechallenge with peanut (7...

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“…Single allergen measurements can help clarify whether cooked egg is tolerated, since ovalbumin (Gal d 2) is signi cantly more heat-labile compared with ovomucoid (Gal d 1) [25,26,27,28,29]. An investigation by Alessandri and coworkers [26] revealed that 44 of 47 Gal d 1-negative patients tolerated cooked egg.…”

Section: Diagnostic Methodsmentioning

confidence: 99%

Cow’s milk and hen’s egg allergy: what do molecular-based allergy diagnostics have to offer?

Reese

Lange

2015

Allergo J Int

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Cow's milk and hen's egg are among the most common triggers of IgE-mediated allergic reactions in childhood. Since not all IgE sensitizations are clinically relevant, it is important to con rm or exclude manifest allergy. ereby the burden of an elimination diet will be restricted to only those cases where such a measure is essential. A number of proteins have been identi ed in cow's milk and hen's egg as single allergens (Bos d 1-12 in cow's milk and Gal d 1-6 in hen's egg). However, measuring IgE to these single allergens does neither predict on clinical relevance nor prognosis of the individual allergy. In terms of ascertaining whether cooked hen's egg is tolerated, measuring sIgE to ovomucoid (Gal d 1) can be useful. Measuring sIgE to lysozyme (Gal d 4) can be helpful to decide whether lysozyme-treated foodstu s (e. g., cheese) should be avoided. Single allergen determination is not able to answer the question of whether cow's milk or hen's egg are tolerated in baked form (e. g., in mu ns). If the foodstu s in question are tolerated in baked form, their regular consumption has a favorable e ect on prognosis. Cite this asReese I, Lange L. Cow's milk and hen's egg allergy: what do molecular-based allergy diagnostics have to o er? Part 20 of the series Molecular allergology. Allergo J Int 2015;24:312-9

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Ovalbumin‐specific IgE/IgG4 ratio might improve the prediction of cooked and uncooked egg tolerance development in egg‐allergic children

Cited by 48 publications

References 36 publications

Development of a microarray-based method for allergen-specific IgE and IgG4 detection

Development of a microarray-based method for allergen-specific IgE and IgG4 detection

Successful immunotherapy induces previously unidentified allergen-specific CD4+ T-cell subsets

Cow’s milk and hen’s egg allergy: what do molecular-based allergy diagnostics have to offer?

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