Ovalbumin antibody-based fluorometric immunochromatographic lateral flow assay using CdSe/ZnS quantum dot beads as label for determination of T-2 toxin

Qie, Zhiwei; Yan, Wenliang; Gao, Zichen; Wu, Meng; Xiao, Rui; Wang, Shengqi

doi:10.1007/s00604-019-3964-x

Cited by 26 publications

(9 citation statements)

References 34 publications

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“…The cross‐reactivity (CR) of TML mAb and other analogues was negligible, which indicated that TML mAb had good specificity for TML. The CR values were calculated using the following equation (Qie et al, 2019; Zhang et al, 2018): CR (%) = (IC 50 of TML/IC 50 of analogue) × 100%.…”

Section: Resultsmentioning

confidence: 99%

“…QDs are semiconductor nanocrystals, which are mainly composed of II–VI or III–V elements (Di Nardo et al, 2016), and can also be composed of two or more elements to form core‐shell structures. QDs have unique optical properties, such as a wide range of ultraviolet (UV) absorption, narrow fluorescence emission spectrum, high fluorescence intensity, long fluorescence lifetime, and high quantum yield (Kozák et al, 2016; Qie et al, 2019; Tian et al, 2010; Zhang et al, 2018). As an ideal fluorescent marker, QDs have attracted more and more attention and have been used in biological and medical immunoassay (Hou et al, 2020; Novikova, Ponomaryova, & Goryacheva, 2020; Qi, Xu, Liu, & Guo, 2020; Yao et al, 2017; Zhou et al, 2021).…”

Section: Introductionmentioning

confidence: 99%

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Quantum dots‐based fluorescence immunoassay for detection of tiamulin in pork

Zhou

Zhang

Qian

et al. 2021

Journal of Food Safety

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Tiamulin (TML) is a kind of diterpenoid veterinary antibiotic. If abused, it will cause residues in edible tissues of animals and endanger human health. Thus, a facile, sensitive, and accurate method is essential for TML detection. In this study, TML complete antigen was synthesized by oximation and active ester method, and monoclonal antibodies with high specificity and sensitivity were prepared. ZnCdSe/ZnS quantum dots (QDs) coupled with goat anti‐mouse IgG were used as a fluorescence detection probe to establish indirect competitive fluorescent immunosorbent assay (icFLISA) for the determination of TML in pork. The 50% inhibitory concentration (IC50) of the icFLISA was 0.069 ng/ml, and the limits of detection (LOD) were 0.309 pg/ml. The recovery of the spiked pork samples for the icFLISA ranged from 85.6 to 96.8%, and the coefficient of variation (CV) was less than 9.4%. The results showed that the icFLISA based on ZnCdSe/ZnS QDs was more sensitive than the traditional indirect competitive enzyme‐linked immunosorbent assay for the detection of TML in edible animal tissues.

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Section: Resultsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Quantum dots‐based fluorescence immunoassay for detection of tiamulin in pork

Zhou

Zhang

Qian

et al. 2021

Journal of Food Safety

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“…The next optimizing experiment was the running buffer, which could substantially affect the immunoreaction of the test lines and the flow rate of the SiO 2 /Au NPs. PBS and 0.5% PBST (PBS containing 0.5% Tween-20, v/v) were considered because our previous work indicated that PBS solution has no influence on the activity of antibody-labeled nanotags (Qie et al, 2019;Wang et al, 2019c). The moderate concentration of Tween-20 ensured the smooth surface of the SiO 2 /Au-bacterial complexes, enabling easy delivery along the strips.…”

Section: Optimization Of Sers-based Lfia Stripsmentioning

confidence: 99%

Rapid, Quantitative, High-Sensitive Detection of Escherichia coli O157:H7 by Gold-Shell Silica-Core Nanospheres-Based Surface-Enhanced Raman Scattering Lateral Flow Immunoassay

Shi

Xiao

et al. 2020

Front. Microbiol.

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Escherichia coli O157:H7 is regarded as one of the most harmful pathogenic microorganisms related to foodborne diseases. This paper proposes a rapiddetection biosensor for the sensitive and quantitative analysis of E. coli O157:H7 in biological samples by surface-enhanced Raman scattering (SERS)-based lateral flow immunoassay (LFIA). A novel gold-shell silica-core (SiO 2 /Au) nanosphere (NP) with monodispersity, good stability, and excellent SERS activity was utilized to prepare highperformance tags for the SERS-based LFIA system. The SiO 2 /Au SERS tags, which were modified with two layers of Raman reporter molecules and monoclonal antibodies, effectively bind with E. coli O157:H7 and form sandwich immune complexes on the test lines. E. coli O157:H7 was quantitatively detected easily by detecting the Raman intensity of the test lines. Under optimal conditions, the limit of detection (LOD) of the SiO 2 /Au-based SERS-LIFA strips for the target bacteria was 50 cells/mL in PBS solution, indicating these strips are 2,000 times more sensitive than colloidal Au-based LFIA strips. Moreover, the proposed assay demonstrated high applicability in E. coli O157:H7 detection in biological samples, including tap water, milk, human urine, lettuce extract and beef, with a low LOD of 100 cells/mL. Results indicate that the proposed SERS-based LFIA strip is applicable for the sensitive and quantitative determination of E. coli O157:H7.

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“…In contrast, immunoassays such as enzyme-linked immunosorbent assay (ELISA) and fluorescence immunoassay assay have the advantages of low cost, short time, and no professional operation. [13,14] Competitive ELISA is one of the most widely used commercial rapid assays because it is economic and sensitive. However, long antibody incubation and washing steps are still required.…”

Section: Introductionmentioning

confidence: 99%

Quantum dot‐based fluorescence immunosorbent assay for the rapid detection of bacitracin zinc in feed samples

et al. 2022

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Bacitracin zinc (BAC), a polypeptide antibiotic, is utilized as a feed additive due to its ability to promote growth in animals. However, the abuse of BAC can lead to a great threat to food safety. Therefore, there is an urgent need to develop a rapid and sensitive detection method. In this study, a monoclonal antibody (mAb) against BAC with excellent sensitivity and specificity was obtained. For the first time, quantum dots (QDs) were conjugated with the prepared mAb against BAC and rabbit anti‐mouse antibody to fabricate a direct and an indirect competitive fluorescence‐linked immunosorbent assay (dc‐FLISA and ic‐FLISA) to detect BAC. The IC50 of dc‐FLISA and ic‐FLISA were 0.28 ng/ml and 0.17 ng/ml, respectively. The limits of detection were 0.0016 ng/ml and 0.001 ng/ml, respectively, and the detection ranges were 0.0016–46.50 ng/ml and 0.001–35.65 ng/ml, respectively. In addition, the recovery rate of the two methods ranged from 93.5% to 112.0%, and the coefficient of variation (CV) was less than 10%. Therefore, the methods developed in this work have the merits of low cost, simple operation, and high sensitivity, which provide an effective analytical tool for BAC residue detection in feed samples.

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Ovalbumin antibody-based fluorometric immunochromatographic lateral flow assay using CdSe/ZnS quantum dot beads as label for determination of T-2 toxin

Cited by 26 publications

References 34 publications

Quantum dots‐based fluorescence immunoassay for detection of tiamulin in pork

Quantum dots‐based fluorescence immunoassay for detection of tiamulin in pork

Rapid, Quantitative, High-Sensitive Detection of Escherichia coli O157:H7 by Gold-Shell Silica-Core Nanospheres-Based Surface-Enhanced Raman Scattering Lateral Flow Immunoassay

Quantum dot‐based fluorescence immunosorbent assay for the rapid detection of bacitracin zinc in feed samples

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