Ovalbumin and Kappa-Carrageenan Mixture Suppresses the Oxidative and Structural Changes in the Myofibrillar Proteins of Grass Carp (Ctenopharyngodon idella) during Frozen Storage

Abstract

This study was done to analyze the cryoprotective influence of ovalbumin (OVA) with kappa-carrageenan (KC) in grass carp myofibrillar proteins during frozen storage. Ca2+-ATPase activity of MP was significantly reduced due to protein denaturation and showed a direct association with decreased sulphydryl (SH) contents and tertiary structural properties. Besides that, an increase in carbonyl, surface hydrophobicity, and dityrosine contents was observed. The addition of OVA-KC significantly restricted the decline in Ca2+-ATPase and SH groups, which were further confirmed by the retarded increase in carbonyls. Furthermore, the addition of OVA-KC increased the stability of α-helix contents. Moreover, MP treated with 6% OVA-KC also improved intermolecular interaction forces linked with gelling and water holding properties of MP. Therefore, it can be concluded that OVA-KC could be used as an effective cryoprotectant in fish and related products for preservation and commercialization.

Hydrogen sulphide and nitric oxide mitigate the negative impacts of waterlogging stress on wheat (Triticum aestivum L.)

“…Sugars have diverse functions in plant metabolism under stress conditions (Walayat et al . 2021). In this context, glucose (reducing sugar) and sucrose (non‐reducing sugar) act as substrates for respiration, as well as osmolytes that maintain cellular homeostasis (Gupta & Kaur 2005).…”

“…The fluctuations in soluble sugar content in plants exposed to environmental stresses is attributed to changes in source-sink carbon partioning, CO 2 assimilation, activity of associated enzymes and expression of related genes (Hassan et al 2021). Sugars have diverse functions in plant metabolism under stress conditions (Walayat et al 2021). In this context, glucose (reducing sugar) and sucrose (non-reducing sugar) act as substrates for respiration, as well as osmolytes that maintain cellular homeostasis (Gupta & Kaur 2005).…”

Quantifying Temperature and Osmotic Stress Impact on Seed Germination Rate and Seedling Growth of Eruca sativa Mill. via Hydrothermal Time Model

“…Moreover, it is rich in health-promoting compounds with numerous medicinal properties [ 1 ]. The different extracts of this plant have been found to have anti-cancer, antioxidant, antithrombotic, and anti-inflammatory properties [ 2 , 3 , 4 , 5 , 6 ]. E. sativa contains a high level of erucic acid, making it a potential source of industrial oils [ 7 ].…”

The role of trifunctional cryoprotectants in the frozen storage of aquatic foods: Recent developments and future recommendations

“…The hydrophobicity and hydrophilicity of the proteins affect their interaction with protein molecules, which causes the change in the three‐dimensional structure of protein molecules called protein denaturation. Moreover, protein denaturation is a significant biochemical change that occurs during freezing and frozen storage of aquatic foods and leads to reduced quality attributes due to decreased Ca 2+ ‐ATPase activity, water retention and gel‐forming abilities (Walayat, Wang, et al., 2021). The degradation of hydrate layers by ice crystals causes protein denaturation, resulting in reduced textural and water binding properties (Walayat, Xiong, Xiong, Moreno, Li, et al., 2020).…”