1991
DOI: 10.1113/expphysiol.1991.sp003535
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Outward currents in rabbit pulmonary artery cells dissociated with a new technique

Abstract: SUMMARYSingle cells from the rabbit pulmonary artery were isolated using a new and convenient procedure. Strips of muscle were incubated overnight in papain at 6°C and dispersed the following morning after warming the tissue for 10 min. This method consistently produced a high yield of relaxed cells, which reversibly responded to vasoconstrictors and remained viable for many hours. The electrophysiological properties of these cells were studied using the patchclamp technique in the whole-cell configuration. In… Show more

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Cited by 112 publications
(94 citation statements)
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“…A voltage-gated K+ current with rapid activation and inactivation kinetics similar to the neuronal fast 'A' current is found in pulmonary artery (Clapp & Gurney, 1991) and portal vein (Beech & Bolton, 1989;Hume & Leblanc, 1989). In large, quiescent arteries such as the aorta, this current may act as a 'brake' to counteract quickly any depolarizing influence.…”
Section: Introductionmentioning
confidence: 99%
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“…A voltage-gated K+ current with rapid activation and inactivation kinetics similar to the neuronal fast 'A' current is found in pulmonary artery (Clapp & Gurney, 1991) and portal vein (Beech & Bolton, 1989;Hume & Leblanc, 1989). In large, quiescent arteries such as the aorta, this current may act as a 'brake' to counteract quickly any depolarizing influence.…”
Section: Introductionmentioning
confidence: 99%
“…Cells were enzymatically isolated by the method of Clapp and Gurney (1991). Cell preparation with this technique is extremely reproducible, and has been used for other vascular smooth muscle cells.…”
Section: Cell Isolationmentioning
confidence: 99%
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“…Single mesenteric artery cells were isolated using an enzymatic dissociation method as described previously, with slight modifications [13] . The mesenteric artery was dissected and placed in dissociation medium containing a low concentration of Ca 2+ (160 μmol/L).…”
Section: Isolation Of Vascular Smooth Muscle Cellsmentioning
confidence: 99%